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Interactions of C4 subtype metabolic activities and transport in maize are revealed through the characterization of dct2 mutants

ABSTRACT: We sequenced mRNA from from four segments along a developing 9 day old third maize leaf of WT W22 accession and ZmDCT2 KO (in W22 background - dct2-1::Ac) plants, to identify changes in gene expression in dct2-1::Ac as a response to the loss of ZmDCT2 function.

ORGANISM(S): Zea mays

PROVIDER: GSE67722 | GEO | 2015/08/30

SECONDARY ACCESSION(S): PRJNA280756

REPOSITORIES: GEO

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ZmDCT2 has a major role in the photosynthetic development of maize

Project description:We sequenced mRNA from from four segments along a developing 9 day old third maize leaf of WT W22 accession and ZmDCT2 KO (in W22 background - dct2-1::Ac) plants, to identify changes in gene expression in dct2-1::Ac as a response to the loss of ZmDCT2 function. The segments were referenced relative to the position of the ligule of leaf 2 as this point represents the source-sink boundary in a wild-type leaf and the transition from shaded leaf tissue to fully light exposed. Segment -4 was isolated at the base of the leaf blade that is photosynthetically inactive, -1, defined as the sink to source transition zone (just below the point of leaf emergence from the whorl), segment +4, from a fully light exposed and photosynthetically active region in the mid-leaf (maturation zone), and +10, the tip of the leaf and the terminally differentiated tissue

2015-08-30 | E-GEOD-67722 | biostudies-arrayexpress

Maize stroma - Reconstruction of metabolic pathways, protein expression, and homeostasis machineries across maize bundle sheath and mesophyll chloroplasts: large-scale quantitative proteomics using the first maize genome assembly

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ZmNF-YB16-YC17-YA1 complex participates in the regulation of plant growth and drought resistance in maize

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Daily temperature cycles promote alternative splicing of RNAs encoding SR45a, a splicing regulator in maize

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Project description:Expression profiling analyses for eight maize inbreds reveals extensive transcriptional variation. This is a companion dataset to an Affymetrix profiling experiment (GEO Series GSE10237). Keywords: Genotype comparison series Expression profiling was used to study gene expression in aerial tissue from 11-day seedlings of maize. Three biological replicates were performed for eight different inbred lines; B37, B73, B84, Mo17, Oh43, B14a, Wf9 and W22.

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Exploring the mechanism of C4 photosynthetic differentiation through a unified comparative analysis of maize and rice leaf transcriptomes

Project description:Maize and rice are the two most economically important grass crops and utilize distinct forms of photosynthesis to fix carbon: C4 and C3 respectively. Relative to C3 photosynthesis, C4 photosynthesis reduces photorespiration and affords higher water and nitrogen use efficiencies under hot arid conditions. To define key innovations in C4 photosynthesis, we profiled metabolites and gene expression along a developing leaf gradient. A novel statistical method was implemented to compare transcriptomes from these two species along a unified leaf developmental gradient and define candidate cis-regulatory elements and transcription factors driving photosynthetic gene expression. We also present comparative primary and secondary metabolic profiles along the gradients that provide new insight into nitrogen and carbon metabolism in C3 and C4 grasses. These resources, including community viewers to access and mine these datasets, will enable the elucidation and engineering of C4 photosynthetic networks to improve the photosynthetic capacity of C3 and C4 grasses. [Maize] Nine day old third leaves of maize were cut into fifteen 1 cm segments; samples were pooled from an average of seven plants per biological replicate and six biological replicates in total were collected on different dates. [Rice] 14 day old third leaves of rice were cut into eleven 2 cm segments, samples were pooled from an average of 15 plants per biological replicate and four replicates in total were collected.

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