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Microarray data obtained from control, cbtRNAi and cbtOE flies

ABSTRACT: Microarray data obtained from control, cbtRNAi (cabut RNAi), and cbtOE (cabut overexpression) flies. From each strain, fly heads at two different time points during the day–night cycle (ZT3 and ZT153) were collected. Fly heads were collected and RNA was extracted. RNA was then used to prepare a probe that was hybridized to Drosophila 2.0 gene expression arrays (Affymetrix).

ORGANISM(S): Drosophila melanogaster

PROVIDER: GSE67865 | GEO | 2015/05/08

SECONDARY ACCESSION(S): PRJNA281092

REPOSITORIES: GEO

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Microarray data obtained from control, cbtRNAi and cbtOE flies

Project description:Microarray data obtained from control, cbtRNAi (cabut RNAi), and cbtOE (cabut overexpression) flies. From each strain, fly heads at two different time points during the day–night cycle (ZT3 and ZT153) were collected. Fly heads were collected and RNA was extracted. RNA was then used to prepare a probe that was hybridized to Drosophila 2.0 gene expression arrays (Affymetrix). Two samples of cbtRNAi, two samples of cbtOE and two samples of the control flies (cyo).

2015-05-08 | E-GEOD-67865 | biostudies-arrayexpress

3' RNA-seq of flies over-expressing cabut protein or RNAi against cabut mRNA

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2015-05-08 | GSE67590 | GEO

3' RNA-seq of flies over-expressing cabut protein or RNAi against cabut mRNA

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2015-05-08 | GSE58854 | GEO

3' RNA-seq of fly expressing cabut RNAi after exposure to different levels of sucrose

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Project description:In order to test whether ADAR is responsible for mediating part of the transcriptional response to temperature changes in fly we determined the gene expression profile of fly heads from control and ADAR Hypomorph flies at 18 and 29°C by 3’ RNA-seq.

2017-07-20 | GSE95313 | GEO

Comparison of Control and Tim-Fbl gene expression profiles

Project description:In order to identify genes affected in our model of PANK defficiency we profiled fly heads of female flies control or expressing fumble RNAi by the use of the tim-gal4 driver Female fly heads of the indicated strains were collected at 2 different circadian timepoints, RNA extracted, labeled and hybridized to Affymetrix oligonucleotide microarrays.

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Identification of genes contributing to a long circadian period in Drosophila melanogaster

Project description:Our objective was to identify candidate genes that contribute to the long 31-hour circadian period previously observed in DGRP_892. We performed transcriptional profiling of whole fly heads from two genotypes: DGRP_892, and Canton-S B, a line with a normal 24-hour circadian period. We collected fly heads every two hours over a 24-hour period. We quantified differential expression among genotype, time, and sex.

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RNA-Seq of female, male, and sex-transformed Drosophila melanogaster heads from flies heterozygous for deletions on chromosome X and 3L

Project description:To measure the response to gene dose, we performed mRNA-Seq of fly heads with molecularly defined deletions constructed from DrosDel deficiency lines (Ryder et al. Genetics 2007, 177(1):615-29) on the Illumina HiSeq 2000 platform.

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AI-facilitated multi-omics reveal TRF-controlled propionylation in determining protein homeostasis

Project description:Firstly, we systematically identified rhythmically modified Kpr sites, through time-series propionylomic profiling of fly heads. Wild-type (WT) and per0 flies were entrained under light/dark cycles (LD) and then released into constant darkness (DD), respectively. On the first day of DD, whole heads of WT flies were collected every 6 hours, including Zeitgeber time 0 (ZT0), ZT6, ZT12 and ZT18, whereas per0 fly heads were collected at ZT6 and ZT18, respectively. For each sample, whole-cell lysates were digested by trypsin and labeled with one of the tandem mass tag (TMT) 6-plex reagents. Then, propionylated peptides were isolated by immuno-affinity enrichment using the pan-propionylation antibody. The 6 samples were mixed with equal amounts and analyzed by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Secondly, metabolomic profiling was conducted using TRF-WT and TRF-H2BK17A fly heads collected at ZT9 and ZT21. After the preprocessing including homogenization and different reagents mixture followed by centrifugation. All samples were analyzed by the LC-MS/MS system. Additionally, Immunoprecipitaion and LC-MS/MS analysis was perform with fly heads using H2BK17pr antibody to evaluate the its enrichment capacity.

2023-03-20 | PXD040987 | iProX

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