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Expression dynamics in mammalian cells responding to protein misfolding stress

ABSTRACT: We have employed whole genome microarray expression profiling to track the transcriptome expression change under DTT induced protein misfolding stress. The relative importance of gene expression regulation at the mRNA versus the protein-level is a matter of ongoing debate. We present an in-depth analysis of a cervical cancer cell line responding to protein misfolding stress induced by dithiothreitol treatment, quantifying the dynamics of mRNA and protein concentrations for >3,500 genes over a time-course 30 hours. While the early phase of the experiment (

ORGANISM(S): Homo sapiens

PROVIDER: GSE67901 | GEO | 2015/08/15

SECONDARY ACCESSION(S): PRJNA281173

REPOSITORIES: GEO

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Expression dynamics in mammalian cells responding to protein misfolding stress

Project description:The relative importance of gene expression regulation at the mRNA versus the protein-level is a matter of ongoing debate. We present an in-depth analysis of a cervical cancer cell line responding to protein misfolding stress induced by dithiothreitol treatment, quantifying the dynamics of mRNA and protein concentrations for >3,500 genes over a time-course 30 hours. While the early phase of the experiment (<two hours) was marked by apoptosis, surviving cells were marked by a strong response to unfolded proteins and stress of the endoplasmatic reticulum, specifically during the intermediate phase (two to eight hours). Using statistical time series analysis, we detected significant changes in the regulatory parameters at the RNA-level, caused by transcription and mRNA degradation, and at the protein-level, caused by translation and protein degradation. mRNA- and protein-level regulation were of equal importance (XX and YYY%, respectively), but displayed different magnitudes and dynamics: mRNA fold changes were much smaller on average than those of the proteins. While our method did not capture immediate changes, we found the strongest regulatory response between two and eight hours after the treatment. mRNA-level regulation showed a spike around this time interval, while protein-level regulation was delayed and continued at slower pace until the end of the experiment.

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Transcriptome and proteome profiling of motor neuron model subjected to protein misfolding stress

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ER, mitochondria and ISR regulation by mt-HSP70 and ATF5 upon procollagen misfolding (proximal tibia srRNASeq)

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Newly translated protein misfolding after heat shock and translation during heat shock

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Homo sapiens

Project description:Expression dynamics in mammalian cells responding to protein misfolding stress

| PRJNA281173 | ENA