Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Next generation sequencing based transcriptomic analysis of bile stress response in Lactobacillus salivarius Ren

ABSTRACT: Lactobacillus salivarius is a member of the indigenous microbiota of the human gastrointestinal tract (GIT). Tolerance to bile stress is crucial for intestinal lactobacilli to survive in the GIT and to exert their beneficial actions. In this work, the Next-Generation Sequencing platform Illumina HiSeq 2000 was used to investigate the global response to bile in L. salivarius Ren, a potential probiotic strain isolated from a healthy centenarian. In the presence of 0.75 g liter-1 oxgall, the transcription of nearly 200 genes was detected to be associated with bile stress, including genes involved in carbohydrate and amino acid metabolism, cell envelope and fatty acid biogenesis, transcription and translation. This study improves our understanding on bile stress response in L. salivarius Ren.

ORGANISM(S): Ligilactobacillus salivarius str. Ren

PROVIDER: GSE68033 | GEO | 2019/12/18

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

RNA sequencing analysis of transcriptomics to study the growth of Lactobacillus salivarius Ren in raffinose

Project description:Purpose: To understand the metabolic mechanism of Lactobacillus salivarius Ren in raffinose Methods: Samples of Lactobacillus salivarius Ren grown in glucose and raffinose were sequenced on the Illumina Hiseq platform. Three independent biological replicates were generated, including a total of six samples. Results: Raw data were firstly processed through in-house perl scripts to generate clean data, and then clean date were mapped to the reference genome, getting about 8-10 million total mapped reads per sample.

2018-11-14 | GSE122483 | GEO

Ligilactobacillus salivarius str. Ren

Project description:Ligilactobacillus salivarius str. Ren Genome sequencing

| PRJNA247452 | ENA

Lactobacillus salivarius str. Ren

Project description:Lactobacillus salivarius str. Ren Genome sequencing

| PRJNA167500 | ENA

Global transcriptomic analysis of the Raffinose Utilization in Lactobacillus salivarius Ren

Project description:This study was aimed to further illustrate the expression files of REN between glucose and raffinose in MRS broth. Transcriptomic analysis combined with mutants of the key genes based on homologous recombination technology indicated that galA1 gene cluster plays an important role in raffinose metabolism. Gene rafP and galA1 are responsible for raffinose transport and α-galactoside hydrolysis, followed by galactose hydrolysis by galKTE and sucrose hydrolysis by scrB. Lactobacillus salivarius Ren expanded the carbon utilization spectrum to adapt the fluctuating carbohydrate sources in the environment and shifted its carbohydrate metabolism to mixed-acid fermentation and then generated extra energy to bacterial growth when exposed to raffinose.

2021-12-31 | GSE174336 | GEO

Transcription profiling of Lactobacillus salivarius bile response_LS201

Project description:Porcine bile induced transcriptome of Lactobacillus salivarius

2009-07-16 | E-MEXP-2224 | biostudies-arrayexpress

Transcription profiling of Lactobacillus salivarius bile response_LS201bsh knockout

Project description:bile induced transcriptome of Lactobacillus salivarius LS201bsh ko.

2009-07-17 | E-MEXP-2225 | biostudies-arrayexpress

Global transcriptomic analysis of the Raffinose Utilization in Lactobacillus salivarius Ren

Project description:Global transcriptomic analysis of the Raffinose Utilization in Lactobacillus salivarius Ren

| PRJNA729477 | ENA

Next generation sequencing based transcriptomic analysis of bile stress response in Bifidobacterium longum BBMN68

Project description:Bifidobacterium longum is one of the natural inhabitants in human gastrointestinal tract. In order to colonize and exert particular functions in the gut, it has to be tolerant to the physiological concentrations of bile salts. In this work, we used RNA-Seq transcriptomics based on the Next-Generation Sequencing to investigate the global response to bile in B. longum BBMN68, a potential probiotic strain isolated from a healthy centenarian. In the presence of 0.75 g liter-1 ox-bile, the transcription of 236 genes were regulated (≥ 3-fold, p < 0.001). Function analysis and Gene Ontology suggested that the bile stress response of B. longum BBMN68 covers almost all biological processes, including bile stress resistance, general stress response, central metabolic process, transmembrane transport, gene expression, cell proliferation and interaction with the host. Remarkably, 3 two-component systems and 11 transcription factors were up- or down-regulated by bile, and target genes of the regulators were identified by bacterial one-hybrid system and bioinformatics methods, resulting in a putative regulatory network that controls bile stress response in B. longum for the first time. This study significantly develops our understanding on bile stress response and brings new insight to the regulatory mechanism in bifidobateria.

2014-06-29 | GSE46446 | GEO

Next generation sequencing based transcriptomic analysis of bile stress response in Bifidobacterium longum BBMN68

Project description:Bifidobacterium longum is one of the natural inhabitants in human gastrointestinal tract. In order to colonize and exert particular functions in the gut, it has to be tolerant to the physiological concentrations of bile salts. In this work, we used RNA-Seq transcriptomics based on the Next-Generation Sequencing to investigate the global response to bile in B. longum BBMN68, a potential probiotic strain isolated from a healthy centenarian. In the presence of 0.75 g liter-1 ox-bile, the transcription of 236 genes were regulated (M-bM-^IM-% 3-fold, p < 0.001). Function analysis and Gene Ontology suggested that the bile stress response of B. longum BBMN68 covers almost all biological processes, including bile stress resistance, general stress response, central metabolic process, transmembrane transport, gene expression, cell proliferation and interaction with the host. Remarkably, 3 two-component systems and 11 transcription factors were up- or down-regulated by bile, and target genes of the regulators were identified by bacterial one-hybrid system and bioinformatics methods, resulting in a putative regulatory network that controls bile stress response in B. longum for the first time. This study significantly develops our understanding on bile stress response and brings new insight to the regulatory mechanism in bifidobateria. Whole mRNA profiles of B. longum BBMN68 growing in the absence (CK) and presence (OG) of ox-bile were generated using AB SOLiD technology and differentially expressed genes were anylyzed.

2014-06-29 | E-GEOD-46446 | biostudies-arrayexpress

RNA sequencing analysis of transcriptomics to study the growth of Lactobacillus salivarius Ren in raffinose

Project description:RNA sequencing analysis of transcriptomics to study the growth of Lactobacillus salivarius Ren in raffinose

| PRJNA505334 | ENA