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Mir775_1st-Role of the young microRNA to plants

ABSTRACT: ra12-08_mir775 - mir775_1st - Look for potential targets of miR775 at Arabidopsis thaliana by the analysis of the transcriptome of a mutant not producing any more the miRNA.

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE68084 | GEO | 2015/04/23

SECONDARY ACCESSION(S): PRJNA281947

REPOSITORIES: GEO

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mir775_1st-Role of the young microRNA to plants

Project description:ra12-08_mir775 - mir775_1st - Look for potential targets of miR775 at Arabidopsis thaliana by the analysis of the transcriptome of a mutant not producing any more the miRNA. 2 dye-swaps - gene knock out.

2015-04-23 | E-GEOD-68084 | biostudies-arrayexpress

Multiple RNA recognition patterns during microRNA biogenesis in plants

Project description:MicroRNAs (miRNAs) are processed from longer precursors with fold-back structures. While animal MIRNA precursors have homogenous structures, plant precursors comprise a collection of fold-backs with variable size and shape. Here, we design an approach (SPARE) to systematically analyze miRNA processing intermediates and characterize the biogenesis of most of the evolutionary conserved miRNAs present in Arabidopsis thaliana. We found that plant MIRNAs are processed by four mechanisms, depending on the sequential direction of the processing machinery and the number of cuts required to release the miRNA. Classification of the precursors according to their processing mechanism revealed specific structural determinants for each group. We found that the complexity of the miRNA processing pathways occurs in both ancient and evolutionary young sequences, and that members of the same family can be processed in different ways. We observed that different structural determinants compete for the processing machinery and that alternative miRNAs can be generated from a single precursor. The results provide a mechanistic explanation for the structural diversity of MIRNA precursors in plants and new insights towards the understanding of the biogenesis of small RNAs. Approach to systematically analyze miRNA processing intermediates and characterize the biogenesis of conserved and young miRNAs present in Arabidopsis thaliana. MiRNA processing intermediates profiles of Wild type and Fiery mutants Arabidopsis plants were analyzed, using Illumina GAIIx.

2013-06-12 | E-GEOD-46429 | biostudies-arrayexpress

Multiple RNA recognition patterns during microRNA biogenesis in plants

2013-06-12 | GSE46429 | GEO

Autophagy role during auxin-induced rapid proteome remodelling

Project description:The aim of the project is to compare NAA-induced proteome remodelling between wild-type plants (Arabidopsis thaliana, Col-0) to autophagy deficient mutants (atg2-1) treated MS growt media suplemented with solvent (EtOH- Control) or NAA (a synthetic variant of the plant hormone auxin. The aim is to look at the rapid response when treated with auxin and as so the treatment is very short at 30 minutes. The time of the experiment was at zeitgeber 0.

2024-04-04 | PXD042834 | Pride

ra12-02_aba-seed-ABA signaling in Arabidopsis seeds

Project description:ra12-02_aba-seed - ra12-02_aba-seed - Identification of ABA signaling factors and targets in Arabidopsis developing seeds by comparing ABA deficient and wild type genotypes - Transcriptome comparison of developing seeds harvested at 10 DAP from two ABA-deficient mutants (nced2 nced5 nced9 and nced2 nced5 nced6 nced9) and wild type (Columbia-0)

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Expression profiles of MicroRNA and SiRNA of Arabidopsis thaliana Col-0 and transgenic plants

Project description:Expression profiles of MicroRNA and SiRNA of Arabidopsis thaliana Col-0 and transgenic plants with constitutive expression of the chimeric receptors NRG1 grown at different temperature To reveal the underlying molecular mechanism of de-cosuppression with memory by high temperature in Arabidopsis, we performed the expression profiles of microRNA and SiRNA in transgenic plants with constitutive expression of the chimeric receptors NRG1 and wide type Col-0 grown at different temperature using the Custom LC Sciences Arabidopsis microRNA and SiRNA array. Keywords: high temperature, de-cosuppression, MicroRNA, SiRNA

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Role of TaVAP in stress tolerance in plants

Project description:Abiotic stresses like drought, salinity, high and low temperature, and submergence are major factors that limit the crop productivity. Hence, identification of genes associated with stress response in crops is a prerequisite for improving their tolerance to adverse environmental conditions. In this study, we have analyzed the expression profiles of three genotypes WT, TaVAP mutant and TaVAPOE plants in Arabidopsis thaliana in col-0 background using microarray technology to identify the genes differentially expressed under control conditions. We used microarrays to detail the global programme of gene expression underlying differential expression and identified distinct classes of up-regulated and downregulated genes during this process.

2018-04-25 | GSE86848 | GEO

Transcription profiling time course experiment performed over 24 hours to look at the effects on gene expression of exposure to low red:far-red ratio light in Arabidopsis thaliana plants

Project description:This experiment was a time course performed over 24 hours to look at the effects on gene expression of exposure to low red:far-red ratio light in Arabidopsis thaliana plants. In this way genes involved in the shade avoidance response might be identified. This experiment was designed for gene identification only and containes no replicates,genes identified were verified by quantitative PCR for publication.

2013-01-30 | E-MEXP-34 | biostudies-arrayexpress

Transcriptional profiling of arabidopsis plants

Project description:au13-08_atpplaii-alpha - transcriptional profilling of arabidopsis plants. Understanding the physiological role of pPLAIIα; under control and drought stress conditions. Study on empty vector, overexpressor and antisense A. thaliana lines.

2016-06-01 | GSE58219 | GEO

Male fertility in plants requires active DNA demethylation of genes that control pollen tube funcion

Project description:We show that in model plant Arabidopsis thaliana, the DNA glycosylases DEMETER (DME) and REPRESSOR OF SILENCING 1 (ROS1) of the model plant Arabidopsis thaliana act semi-redundantly in pollen for hypomethylation of the vegetative cell, required for the activation of key genes involved in pollen tube function

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