Genomics

Dataset Information

0

MiRNA expression profiles of TIE2 expressing Monocytes (TEMs)


ABSTRACT: To identify the miRNA expressing profiles of TIE2 expressing Monocytes (TEMs), we have employed the Agilent Human miRNA 8×60K (Design ID:046064) microarray. Human peripheral blood mononuclear cells (PBMCs) in venous blood from healthy donors were isolated by Lymphoprep (Axis-Shield, Norway). Human monocytes in PBMCs, identified as cells that expressed CD14, were enriched by positive immunomagnetic selection using anti-CD14 MicroBeads (Miltenyi, Germany). TEMs (TIE2+CD14+) and TIE2-Monocytes (Tie2-CD14+) were then isolated by FACS-sorting (Aria II, BD Biosciences) using FITC-conjugated anti-CD14 (BD Biosciences, USA) and APC-conjugated anti-TIE2 (R&D System, USA) antibodies.Three TEMs samples together with their paried TIE2-Monocytes were detected.

ORGANISM(S): Homo sapiens

PROVIDER: GSE68322 | GEO | 2017/05/19

SECONDARY ACCESSION(S): PRJNA282423

REPOSITORIES: GEO

Similar Datasets

2017-05-17 | GSE57783 | GEO
| E-GEOD-57783 | biostudies-arrayexpress
2014-12-31 | GSE61399 | GEO
2022-10-16 | GSE215451 | GEO
| E-GEOD-31465 | biostudies-arrayexpress
2020-06-13 | GSE152387 | GEO
2022-03-15 | GSE198616 | GEO
2020-12-09 | GSE155719 | GEO
| E-GEOD-70327 | biostudies-arrayexpress
2022-06-08 | GSE181250 | GEO