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Conservation of m5C in rRNA in the kingdom Plantae

ABSTRACT: Here we use bisulfite conversion of RNA combined with high-throughput IIlumina sequencing (RBS-seq) to identify single-nucleotide resolution of m5C sites in non-coding ribosomal RNAs of all three sub-cellular transcriptomes across six diverse species that included, the single-celled algae Nannochloropsis oculata, the macro algae Caulerpa taxifolia and multi-cellular higher plants Arabidopsis thaliana, Brassica rapa, Triticum durum and Ginkgo biloba. RNA-seq was performed on total RNA for all species except for Arabidopsis in order to generate rRNA reference sequences using the Arabidopsis rRNA sequences (TAIR10) as a guide.

ORGANISM(S): Ginkgo biloba Arabidopsis thaliana Caulerpa taxifolia Brassica rapa Nannochloropsis oculata Triticum turgidum subsp. durum

PROVIDER: GSE68447 | GEO | 2015/10/01

SECONDARY ACCESSION(S): PRJNA282748

REPOSITORIES: GEO

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