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Differential microRNA expression profiling between human induced pluripotent stem cells (iPSCs) and human neonatal dermal fibroblasts (NDFs).

ABSTRACT: We have employed microRNA expression profiling of iPSCs and NDFs as a discovery platform to identify microRNAs involved in reprogramming of NDFs to iPSCs.

ORGANISM(S): Homo sapiens

PROVIDER: GSE68672 | GEO | 2015/05/09

SECONDARY ACCESSION(S): PRJNA283354

REPOSITORIES: GEO

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Differential microRNA expression profiling between human induced pluripotent stem cells (iPSCs) and human neonatal dermal fibroblasts (NDFs).

Project description:We have employed microRNA expression profiling of iPSCs and NDFs as a discovery platform to identify microRNAs involved in reprogramming of NDFs to iPSCs. Using Agilent human miRNA (V3) 8X15K microarray, we performed microRNA profiling of iPSCs at passage 20 and NDFs at passage 5

2015-05-09 | E-GEOD-68672 | biostudies-arrayexpress

Characterization of Human Induced Pluripotent Stem Cell Lines Derived from Neonatal and Adult Human Dermal Fibroblasts

Project description:Somatic cells can be reprogrammed to Induced Pluripotent Stem Cells (iPSCs) by expressing four transcription factors, Oct4, Sox2, Klf4 and c-Myc. Co-expressing Rarg (retinoic acid receptor gamma) and Lrh-1 (liver receptor homolog 1, Nr5a2) with the four factors greatly accelerated reprogramming so that reprogramming of mouse embryonic fibroblast cells (MEFs) to ground state iPSCs requires only four days’ induction of these six factors. The six-factor combination readily reprogrammed primary human neonatal and adult fibroblast cells to exogenous-factor-independent iPSCs, which resembled ground state mouse ES cells in growth properties, gene expression and signalling dependency. Our findings demonstrate that signalling through RARs has critical roles in molecular reprogramming and the synergistic interaction between Rarg and Lrh1 directs reprogramming towards ground state pluripotency. SH-iPS20-1, -3 and -9 lines were derived from HDFa (439656), SH-iPS24-1, -2 and -11 lines were derived from HDFa (709590), and SH-iPS28-23, -25 and -27 lines were derived from HDFn (617769). Parental HDF lines were used as control. SH-iPS15-5 and -10 lines were derived from HDFn (617769, Invitrogen). H1 hESC were obtained from Wicell (Madison, USA). SH-iPSCs were cultured in both 2i+LIF and FGFconditions. Expression pattern of SH-iPSCs was compared to H1 hESC cultured in FGF conditions. S1, S2 and S3 are three indpendent subcultures of a cell line.

2011-10-03 | E-GEOD-32581 | biostudies-arrayexpress

Characterization of Human Induced Pluripotent Stem Cell Lines Derived from Neonatal and Adult Human Dermal Fibroblasts

2011-10-04 | GSE32581 | GEO

Generation of human induced pluripotent stem cells from dermal fibroblasts.

Project description: Not available

| S-EPMC2268554 | biostudies-literature

Gene Signature of Human Oral Mucosa Fibroblasts: Comparison with Dermal Fibroblasts and Induced Pluripotent Stem Cells.

Project description: Not available

| S-EPMC4538314 | biostudies-literature

Human induced pluripotent stem cells (iPSCs) derived from T-cell compared with that from adult dermal fibroblast (aHDF) [gene expression]

Project description:In this study we determine the transcriptional profile by microarray of iPSCs and iPSC-derived neurospheres generated from T-cells or aHDF by using direct neurosphere method. iPSCs and iPSC-derived tertiary neurospheres generated from T-cells or aHDF subjected to the gene expression microarray analysis.

2016-01-14 | E-GEOD-76830 | biostudies-arrayexpress

Gene expression profiles of induced pluripotent stem cells (iPSCs) and skin fibroblasts from orangutans

Project description:Orangutans are an endangered species whose natural habitats are restricted to the Southeast Asian islands of Borneo and Sumatra. For potential species conservation and functional genomics studies, we derived induced pluripotent stem cells (iPSCs) from cryopreserved skin fibroblasts obtained from captive orangutans. We report the gene expression profiles of iPSCs and skin fibroblasts derived from orangtuans. The overall goal was to evaluate gene expression biomarkers of pluripotency in iPSCs and skin fibroblasts derived from PBD-ZSD patients and healthy controls. Dermal fibroblast cultures from 2 orangutans were reprogrammed into iPSCs by transfection with retroviruses designed to express the human OCT4, SOX2, KLF4 and c-MYC cDNA. Fibroblasts and iPSCs were cultured in 1:1 ratio of DMEM:F12 medium supplemented with 20% KOSR (knock-out serum replacement) at 37°C with 5% CO2 until confluence for RNA extraction. The overall goal was to evaluate gene expression biomarkers of pluripotency in iPSCs and original fibroblast cultures.

2015-06-10 | E-GEOD-69603 | biostudies-arrayexpress

Matrisome of neonatal mouse dermal fibroblasts

Project description:The goal of this project is to compare the protein composition of the extracellular matrices (ECMs) deposited in vitro by wild type, heterozygous, or SPAG17 KO neonatal mouse dermal fibroblasts.

2022-09-22 | PXD019869 | Pride

LC-MSMS of the MHC-I immunopeptidome of human iPSCs

Project description:The development of cancer immunotherapeutics is limited by the discovery of actionable tumor-specific antigens (TSAs), which need to be presented at high levels on cancer cells, but not presented by normal tissues or presented at levels below the threshold for T-cell recognition to prevent autoimmunity. Accumulating evidence indicates that pluripotent stem cells (PSCs), namely embryonic stem cells (ESCs) and induced PSCs (iPSCs), express a class of pluripotency-associated MHC-I-associated peptides (paMAPs) that can be recognized by tumor-specific T cells. Hence, being strictly confined to the embryonic stage of development and absent from healthy adult tissues, paMAPs shared by iPSCs and tumors would represent ideal targets for the development of TSA-based vaccines against a wide range of cancers. We performed immunopeptidomic analyses of human iPSCs and we identified a set of paMAPs that could serve for the design of TSA-based vaccines against many cancers.

2022-08-22 | PXD026702 | Pride

Human induced pluripotent stem cells (iPSCs) derived from T-cell compared with that from adult dermal fibroblast (aHDF) [gene expression]

Project description:In this study we determine the transcriptional profile by microarray of iPSCs and iPSC-derived neurospheres generated from T-cells or aHDF by using direct neurosphere method.

2016-01-14 | GSE76830 | GEO

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