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Expression data of proliferating and differentiating ENS progenitor cells

ABSTRACT: Postnatal neural progenitors of the enteric nervous system are a potential source for future cell replacement therapies of developmental dysplasia like Hirschsprung's disease. However, little is known about the molecular mechanisms driving the homeostasis and differentiation of this cell pool. In this work, we conducted Affymetrix gene chip experiments to identify differences in gene regulation between proliferation and early differentiation of enteric neural progenitors. We detected a total of 1333 regulated genes that were linked to different groups of cellular mechanisms involved in cell cycle, apoptosis, neural proliferation, and differentiation. As expected, we found a strong inhibition of cell cycle progression as well as an enhanced expression of neuronal and glial markers. We further found a marked inactivation of the canonical Wnt pathway during the beginning of cellular differentiation. Taken together, this data illustrated the various mechanisms taking place during the proliferation and early differentiation of enteric neural progenitor cells.

ORGANISM(S): Mus musculus

PROVIDER: GSE68797 | GEO | 2015/05/13

SECONDARY ACCESSION(S): PRJNA283841

REPOSITORIES: GEO

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Expression data of proliferating and differentiating ENS progenitor cells

Project description:Postnatal neural progenitors of the enteric nervous system are a potential source for future cell replacement therapies of developmental dysplasia like Hirschrpung’s disease. However, little is known about the molecular mechanisms driving the homeostasis and differentiation of this cell pool. In this work, we conducted Affymetrix gene chip experiments to identify differences in gene regulation between proliferation and early differentiation of enteric neural progenitors. We detected a total of 1333 regulated genes that were linked to different groups of cellular mechanisms involved in cell cycle, apoptosis, neural proliferation, and differentiation. As expected, we found a strong inhibition of cell cycle progression as well as an enhanced expression of neuronal and glial markers. We further found a marked inactivation of the canonical Wnt pathway during the beginning of cellular differentiation. Taken together, this data illustrated the various mechanisms taking place during the proliferation and early differentiation of enteric neural progenitor cells. We analyzed 2 groups with 3 samples each: 1 group consistent of cells proliferating für 11 days and 1 group of enterospheres proliferating for 9 days and differentiating for 2 days before RNA extraction

2015-05-13 | E-GEOD-68797 | biostudies-arrayexpress

RNA Expression Data from developing enteric nervous system (ENS) and bowel

Project description:The enteric nervous system (ENS) can control most essential gut functions owing to its organization into complete neural circuits consisting of a multitude of different neuronal subtypes. We used microarrays to identify transcription factor networks and signaling pathways involved in diversification and differentiation of enteric neurons during development of the enteric nervous system.

2017-12-05 | GSE100130 | GEO

A branching model of lineage differentiation underpinning the neurogenic potential of enteric glia (Single-cell expression analysis of small intestine enteric nervous system developmental data)

Project description:Glial cells have been proposed as an endogenous source of progenitors for the treatment of neural deficits. However, the cellular and molecular mechanisms underpinning the neurogenic potential of certain populations of adult glial cells, are not known. Using single cell transcriptomic profiling, we show here that enteric glial cells represent a cell state attained by autonomic neural crest cells as they transition during development along a linear default differentiation trajectory that allows them to retain neurogenic potential while acquiring a gene expression profile associated with their role in neuronal support and immunomodulation. Key neurogenic loci in early enteric nervous system progenitors remain in open chromatin configuration in mature enteric glia, thus facilitating neuronal differentiation under appropriate conditions. Molecular profiling and gene targeting of enteric glial cells in a novel cell culture system of enteric neurogenesis and a gut injury model, demonstrated that neuronal differentiation of glia is driven by transcriptional programs employed in vivo by early progenitors. Our work provides mechanistic insight into the dynamic regulatory landscape underpinning the development of intestinal neural circuits and generates a platform for advancing glial cells as therapeutic agents for the treatment of neural deficits.

2023-07-23 | GSE237713 | GEO

A branching model of lineage differentiation underpinning the neurogenic potential of enteric glia (Single-cell expression analysis of neurogenic enteric glial cell cultures)

2023-07-26 | GSE237970 | GEO

A branching model of lineage differentiation underpinning the neurogenic potential of enteric glia (bulk ATAC-seq data from ganglioid cultures)

2023-09-01 | GSE241522 | GEO

A branching model of lineage differentiation underpinning the neurogenic potential of enteric glia (Single-cell ATAC-seq data for enteric glial cells)

2023-07-31 | GSE239305 | GEO

Influence of 3,5,3'-Triiodothyronine (T3) on differentiating progenitor cells of the enteric nervous system (ENS)

Project description:Differentiation assays with neural progenitor cells of the enteric nervous system (ENS) showed elongated neurite outgrowth under influence of 3,5,3'-Triiodothyronine (concentrations 50 nm and 100 nm). For analysis, neural cells were stained with TUJ1 (beta-Tubulin III). Microarray analysis should enlighten these results on a genetical basis and give hints about the regulation pathways.

2013-09-23 | GSE39634 | GEO

Influence of 3,5,3'-Triiodothyronine (T3) on differentiating progenitor cells of the enteric nervous system (ENS)

2013-09-23 | E-GEOD-39634 | biostudies-arrayexpress

Comparative Microarray Analysis of Proliferating and Differentiating Murine ENS Progenitor Cells.

Project description: Not available

| S-EPMC4677255 | biostudies-literature

The Transcription factor NF-YA is Crucial for Neural Progenitor Maintenance during Brain Development

Project description:In contrast to stage-specific transcription factors, the role of ubiquitous transcription factors in neuronal development remains a matter of scrutiny. Here, we demonstrated that a ubiquitous factor NF-Y is essential for neural progenitor maintenance during brain morphogenesis. Deletion of the NF-YA subunit in neural progenitors by using nestin-cre transgene in mice resulted in significant abnormalities in brain morphology, including a thinner cerebral cortex and loss of striatum during embryogenesis. Detailed analyses revealed a progressive decline in multiple neural progenitors in the cerebral cortex and ganglionic eminences, accompanied by induced apoptotic cell death and reduced cell proliferation. In neural progenitors, the NF-YA short isoform lacking exon 3 is dominant and co-expressed with cell cycle genes. ChIP-seq analysis from the cortex during early corticogenesis revealed preferential binding of NF-Y to the cell cycle genes, some of which were confirmed to be downregulated following NF-YA deletion. Notably, the NF-YA short isoform disappears and is replaced by its long isoform during neuronal differentiation. Forced expression of the NF-YA long isoform in neural progenitors resulted in a significant decline in neuronal count, possibly due to the suppression of cell proliferation. Collectively, we elucidated a critical role of the NF-YA short isoform in maintaining neural progenitors, possibly by regulating cell proliferation and apoptosis. Moreover, we identified an isoform switch in NF-YA within the neuronal lineage in vivo, which may explain the stage-specific role of NF-Y during neuronal development.

2024-01-11 | GSE244758 | GEO

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