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Expression data from -Pi treatment between WT, phr1, phr3 and phr1phr2phr3 triple mutant

ABSTRACT: Fourteen days plants growth under hydroponic +P condition (200 μM) were treated under –P (no phosphate) condition for another 7 days, shoot of plants from 3 biological repeats were sampled for Affymetrix microarray analysis. We used microarrays to detail the global programme of gene expression underlying -Pi condition among WT and mutants of phr1, phr3 and triple mutant of phr1phr2phr3.

ORGANISM(S): Oryza sativa

PROVIDER: GSE69010 | GEO | 2015/06/25

SECONDARY ACCESSION(S): PRJNA284281

REPOSITORIES: GEO

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Expression data from -Pi treatment between WT, phr1, phr3 and phr1phr2phr3 triple mutant

Project description:Fourteen days plants growth under hydroponic +P condition (200 μM) were treated under –P (no phosphate) condition for another 7 days, shoot of plants from 3 biological repeats were sampled for Affymetrix microarray analysis. We used microarrays to detail the global programme of gene expression underlying -Pi condition among WT and mutants of phr1, phr3 and triple mutant of phr1phr2phr3. Fourteen days plants growth under hydroponic +P condition (200 μM) were treated under –P (no phosphate) condition for another 7 days, shoot of plants from 3 biological repeats were sampled for Affymetrix microarray analysis.

2015-06-25 | E-GEOD-69010 | biostudies-arrayexpress

Expression data from +Pi and -Pi treeatment between WT and spx1spx2 double mutant

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Expression data from Arabidopsis thaliana PHR1 primary targets genes under phosphate starvation stress

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Directional RNA-Seq analysis of Arabidopsis plants grown under Pi-deprived conditions

Project description:Directional RNA-Seq analysis of Arabidopsis plants grown under Pi-deprived conditions PolyA+ RNA isolated from Col-0, hho2-1, phr1 and hho2phr1 grown on agar-solidified P- condition for 7d

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Expression data from Arabidopsis thaliana under phosphate starvation stress

Project description:We performed a transcriptomic analysis of Pi starvation responses in Arabidopsis thaliana (Columbia-0) wild type plants under phosphate starvation stress and in plants with altered PHR1(-like) activity, comparing mutants of phr1 and phr1-phl1 grown in phosphate-lacking medium. Results show the central role of PHR1 and functionally redundant members of its family in the control of transcriptional responses to Pi starvation. The analysis was performed in wild-type plants grown for seven days in complete (+Pi) and Pi-lacking (-Pi) Johnson solid media and the single phr1 and double phr1-phl1 mutants grown for 7 days in –Pi medium. Three independent biological samples of total RNA from shoot and root were hybridized separately.

2010-12-09 | E-GEOD-16722 | biostudies-arrayexpress

Expression data from Arabidopsis thaliana under phosphate starvation stress

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An improved, low-cost, hydroponic system for growing Arabidopsis and other model plant species under sterility conditions (Arabidopsis transcriptional profile in early response to Pi starvation)

Project description:Transcriptional profiling of roots from 12 days-old Arabidopsis thaliana seedlings transfered to Pi-deficient medium in hydroponic solution in a 10 min, 30 min, and 2 hrs time course period.

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Expression data from Col-0 and sp1,spx2 under phosphate starvation stress and recovery after resupplying phosphate

Project description:We performed a transcriptomic analysis of Pi-starvation and recovery after resupplying Pi in Arabidopsis thaliana (Columbia-0) wild type plants and double mutant spx1,spx2. Results show that SPX1 is a Pi-dependent inhibitor of the transcription factor PHR1, a central regulatory protein in the control of transcriptional responses to Pi starvation. The analysis was performed in wild-type and double spx1,spx2 double mutant plants grown for seven days in complete (+Pi) and Pi-lacking (-Pi) Johnson solid media. Then, plants were transferred to complete medium for 4 hours to analyze the effect of resupplying plants with Pi.

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