Transcriptomics,Genomics

Dataset Information

30

Transcriptional responses of Pseudomonas veronii 1YdBTEX2 to the exposure to laboratory induced matric and solute stresses


ABSTRACT: We study the transcriptional reactions of bacteria interesting for biodegradation under laboratory conditions that mimic water stress. We compared the transcriptomes of cultures growing exponential phase under optimal conditions versus their responses to an osmotic shock of 30 min in exponential phase. The osmotic shock consisted in a reduction of water potential induced by salt (NaCl, solute stress) or by polyethylene glycol (PEG8000, matric stress). The stress was such that cells are not more than 20% affected in their maximum growth rate. Overall design: The purpose of this study is to compare the responses of Pseudomonas veronii 1YdBTEX2 under solute and matric shock stresses (30min of exposition). These transcriptome responses will be compared with those experiences by other strain potentially useful for bioremediation in order to understand better their behaviour in inoculations in soil.

INSTRUMENT(S): Agilent-038776 P_veroni_YdBTEX

SUBMITTER: Silvia K. Moreno-Forero  

PROVIDER: GSE69022 | GEO | 2015-12-01

SECONDARY ACCESSION(S): PRJNA284335

REPOSITORIES: GEO

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Publications

Comparison of differential gene expression to water stress among bacteria with relevant pollutant-degradation properties.

Moreno-Forero Silvia K SK   Rojas Edward E   Beggah Siham S   van der Meer Jan R JR  

Environmental microbiology reports 20160127 1


Resistance to semi-dry environments has been considered a crucial trait for superior growth and survival of strains used for bioaugmentation in contaminated soils. In order to compare water stress programmes, we analyse differential gene expression among three phylogenetically different strains capable of aromatic compound degradation: Arthrobacter chlorophenolicus A6, Sphingomonas wittichii RW1 and Pseudomonas veronii  1YdBTEX2. Standardized laboratory-induced water stress was imposed by shock  ...[more]

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