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Comparison of gene expresson between larger sized tetraploid and smaller sized diploid HCA2

ABSTRACT: In order to determine the factors that regulate senescence induction, we tried to identfy genes that are predominantly expressed in large sized senescent cells with tetraploid DNA.

ORGANISM(S): Homo sapiens

PROVIDER: GSE69116 | GEO | 2015/05/22

SECONDARY ACCESSION(S): PRJNA284597

REPOSITORIES: GEO

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Comparison of gene expresson between larger sized tetraploid and smaller sized diploid HCA2

Project description:In order to determine the factors that regulate senescence induction, we tried to identfy genes that are predominantly expressed in large sized senescent cells with tetraploid DNA. We sorted large or small sized HCA2 cells treated wth IR (10Gy) according to size and structure (FSC and SSC) by FACScan and analyzed global gene expression patterns.

2015-05-22 | E-GEOD-69116 | biostudies-arrayexpress

DLD1 Diploid vs Tetraploid

Project description:Tetraploidisation has been implicated in tumorigenesis and in aneuploidy which is frequently observed in most human cancers. In this study we aim to reveal differences in gene expression profile of p53 defective isogenic diploid and tetraploid cell populations. Isogenic DLD1 populations were obtained by transient Dihydro-Cytochalasin B treatment and cell sorting of pure diploid and tetraploid populations by cell cycle profile several days after release from DCB. RNA isolation: Two technical replicas were collected for each population from two different passages (biological replicas) of 60-80% confluent cells.

2014-04-22 | E-MTAB-1789 | biostudies-arrayexpress

Gene expression profile of diploid and tetraploid mouse primary hepatocyte

Project description:Genetically unstable tetraploid cells can promote tumorigenesis. Recent estimates suggest that ~37% of human tumors have undergone a genome-doubling event during their development. This potentially oncogenic effect of tetraploidy is countered by a p53-dependent barrier to proliferation. However, the cellular defects and corresponding signaling pathways that trigger growth suppression in tetraploid cells are not known. Here we combine genome-scale RNAi screening and in vitro evolution approaches to demonstrate that cytokinesis failure activates the Hippo tumor suppressor pathway in cultured cells as well as in naturally occurring tetraploid cells in vivo. Induction of the Hippo pathway is triggered in part by extra centrosomes, which alter small G-protein signaling and activate LATS2 kinase; LATS2 in turn stabilizes p53 and inhibits the transcriptional regulators YAP and TAZ. These findings define an important tumor suppression mechanism. Furthermore, our experiments uncover adaptations that allow nascent tumor cells to bypass this inhibitory regulation. Hepatocytes of newborn mice are initially diploid, but become progressively tetraploid with age. To study the mechanism of cell cycle arrest in naturally occurred tetraploidy, we used microarrays to detail the overall gene expression profile of diploid and tetraploid primary hepatocytes and identified YAP target genes up-regulated in diploid cells.

2017-02-10 | GSE57769 | GEO

Comparison of gene expression profiles in diploid and transformed tetraploid MEF cells

2012-04-30 | GSE18802 | GEO

Transcription profiling of diploid and tetraploid yeast cell cultures

Project description:Diploid and tetraploid budding yeast cell cultures were grown in YPD, at 30C, to O.D. approx. 0.5.

2006-11-09 | E-MEXP-822 | biostudies-arrayexpress

Gene expression in diploid and evolved tetraploid RPE-1 and BJ-1 cells

Project description:Both diploid RPE-1 and BJ-1 cells were made tetraploid by transient treatment with the cytokinesis inhibitor DCD. Proliferating tetraploids from both BJ-1 and RPE-1 were selected and isolated. The gene expression profiles of the proliferating tetraploid cells were then compared to the diploids from which they originated.

2014-05-22 | GSE57864 | GEO

Comparison of gene expression profiles in diploid and transformed tetraploid MEF cells

Project description:We propose that abnormal DNA content in tetraploid cells can alter the gene expression profile, contributing to the cellular transformation. To test this hypothesis, we employ mRNA microarray to compare the gene expression profile in transformed tetraploid cells with that in the parent diploid cells. Keywords: gene expression profile determination Total RNA were extracted from diploid or tetraploid cells (n = 3 for each cell lines. mRNA microarray hybridization was conducted. Data were collected and analyzed.

2012-04-29 | E-GEOD-18802 | biostudies-arrayexpress

Watermelon Proteome - To determine the differential expression of proteins in leaf and fruit of diploid and tetraploid watermelon

Project description:To determine the differential expression of proteins in leaf and fruit of diploid and tetraploid watermelon

2019-11-11 | PXD005179 | Pride

Comparison of diploid and transformed tetraploid MEF cells: gene expression and DNA methylation profiling

Project description:This SuperSeries is composed of the following subset Series: GSE18802: Comparison of gene expression profiles in diploid and transformed tetraploid MEF cells GSE18814: Comparison of DNA methylation profiles in diploid and transformed tetraploid MEF cells Refer to individual Series

2012-04-29 | E-GEOD-18815 | biostudies-arrayexpress

Gene expression in diploid and evolved tetraploid RPE-1 and BJ-1 cells

2014-05-22 | E-GEOD-57864 | biostudies-arrayexpress

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