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Seasonal gene expression kinetics between diapause phases in Drosophila virilis group speciesand overwintering differences between diapausing and non-diapausing females

ABSTRACT: Most northern insect species experience a period of developmental arrest, diapause, which enables them to survive over the winter and postpone reproduction until favorable conditions. We studied the timing of reproductive diapause and its long-term effects on the cold tolerance of Drosophila montana, D. littoralis and D. ezoana females in seasonally varying environmental conditions. At the same time we traced expression levels of 219 genes in D. montana using a custom-made microarray. We show that the seasonal switch to reproductive diapause occurs over a short time period, and that overwintering in reproductive diapause has long-lasting effects on cold tolerance. Some genes, such as Hsc70, Jon25bi and period, were upregulated throughout the diapause, while others, including regucalcin, couch potato and Thor, were upregulated only at its specific phases. Some of the expression patterns induced during the sensitive stage, when the females either enter diapause or not, remained induced regardless of the later conditions. qPCR analyses confirmed the findings of the microarray analysis in D. montana and revealed similar gene expression changes in D. littoralis and D. ezoana. The present study helps to achieve a better understanding of the genetic regulation of diapause and of the plasticity of seasonal responses in general.

ORGANISM(S): Drosophila montana Drosophila virilis

PROVIDER: GSE69202 | GEO | 2015/06/03

SECONDARY ACCESSION(S): PRJNA285528

REPOSITORIES: GEO

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Seasonal gene expression kinetics between diapause phases in Drosophila virilis group speciesand overwintering differences between diapausing and non-diapausing females

Project description:Most northern insect species experience a period of developmental arrest, diapause, which enables them to survive over the winter and postpone reproduction until favorable conditions. We studied the timing of reproductive diapause and its long-term effects on the cold tolerance of Drosophila montana, D. littoralis and D. ezoana females in seasonally varying environmental conditions. At the same time we traced expression levels of 219 genes in D. montana using a custom-made microarray. We show that the seasonal switch to reproductive diapause occurs over a short time period, and that overwintering in reproductive diapause has long-lasting effects on cold tolerance. Some genes, such as Hsc70, Jon25bi and period, were upregulated throughout the diapause, while others, including regucalcin, couch potato and Thor, were upregulated only at its specific phases. Some of the expression patterns induced during the sensitive stage, when the females either enter diapause or not, remained induced regardless of the later conditions. qPCR analyses confirmed the findings of the microarray analysis in D. montana and revealed similar gene expression changes in D. littoralis and D. ezoana. The present study helps to achieve a better understanding of the genetic regulation of diapause and of the plasticity of seasonal responses in general. Custom made DNA microarray for Drosophila montana and D. virilis. Current experiment includes 8 samples (7 to 250 days old diapausing or non-diapausing D. montana females) with two or three biological replicates

2015-06-03 | E-GEOD-69202 | biostudies-arrayexpress

Gene expression in Drosophila montana

Project description:We created a custom-made microarray for D. montana with 101 genes known to affect traits important in diapause, photoperiodism, reproductive behaviour, circadian clock and stress tolerance in model Drosophila species. This array gave us a chance to filter out genes showing expression changes during photoperiodic reproductive diapause in a species adapted to live in northern latitudes with high seasonal changes. The three samples for the microarray experiments included young females (âyoungâ), 14 days old females cultured under diapause-preventing (âreproducingâ) and 14 days old females cultured under diapause-inducing conditions (âdiapausingâ). Each of these samples had three replicates. The microarray contained 197 unique probe sequences representing 101 candidate genes and seven housekeeping genes. Some of the probes had two copies per array, in which case the averages of their signals were calculated. The Agilent control probes included 77 negative control probes and 200 spike-in probes (10 different probe types, each represented by 20 copies per array).

2010-05-06 | E-GEOD-18475 | biostudies-arrayexpress

Gene expression in Drosophila montana

2010-03-04 | GSE18475 | GEO

Role of hormones regulate productive diapause queens in year-round mass rearing of Bombus terrestris

Project description:Adult reproductive diapause is a powerful overwintering strategy for many continental insect species including bumblebees, which enables queens to survive several months through harsh winter conditions and then build new beehives in the following spring. There are few reports regarding the molecular regulatory mechanism of reproductive diapause in Bombus terrestris, which is an important pollinators of wild plants and crops, and our previous researches identified the conditions for reproductive diapause of year-round mass rearing. Here, we performed combined RNA sequencing transcriptomics and quantitative proteomic analyses in different development phases relate to reproductive diapause. According to the overall analysis, we found these differentially expressed proteins/genes act in the citrate cycle, insect hormone biosynthesis, insulin and mTOR signalling pathway. To get better sense of the reproductive diapause regulated mechanism, some genes regulated JH synthesis, insulin/ TOR signal pathway were detected, the BtRheb, BtTOR, BtVg and BtJHAMT had lower expression levels in diapause queens, and the JH III titers levels and some metabolic enzymes activities were significantly up-regulated in found post-diapause queens. After microinjected insulin-like peptides (ILPs) and JH analog (JHA), some indicators shows the significantly changes of hormones, cold tolerance substances, metabolic enzymes and reproduction. Along with other related researches, a reproductive diapause regulated model during B. terrestris year-round mass rearing process was establishment. This study contribute to a comprehensive view and the molecular regulate mechanism of productive diapause in eusocial insect.

2021-04-06 | PXD017616 | Pride

Differential expression of microRNA associated with photoperiod in Drosophila

Project description:In Drosophila, the rapid shortening of the day during the autumn induces an arrest of ovarian development (diapause) in females. The molecular basis underlying this photoperiodic seasonal response is yet unknown. Here we analyse the global expression changes in transcripts and microRNA (miRNA) associated with photoperiodic diapause response, focusing on the head transcriptome as the putative location of the photoperiodic timer.

2014-07-18 | GSE28246 | GEO

Gene expression in Drosophila female heads during diapause.

Project description:Young Drosophila females respond to low temperature and short photoperiod by developmental arrest of the ovaries. This form of reproductive diapause is a winter adaptation. Here we used Affymetrix microarrays to study global expression in female heads associated with diapause.

2014-07-18 | GSE28370 | GEO

Photoperiodic induction of gene expression in Drosophila.

2014-07-18 | GSE28588 | GEO

Genome-wide transcriptome effects of reproductive diapause in Drosophila melanogaster.

Project description:The study describes differential expression of genes in adult female flies (Canton S) kept for three weeks in reproductive diapause, compared to ones kept in normal conditions. It discusses signal pathways that are activated or inactivated during diapause and how this relates to the general diapause phenotype with arrested vitellogenesis, extended lifespan, decreased somatic senescence, increased stress resistance and reallocated energy resources.

2016-01-20 | E-MTAB-3546 | biostudies-arrayexpress

Gene Expression during the Egg Development of Drosophila melanogaster.

Project description:Despite the importance of egg development to the female life cycle in Drosophila, global patterns of gene expression have not been examined in detail primarily due to the difficulty of synchronizing developmental stages. Entry into vitellogenesis is however an key stage of oogenesis, and by delaying entry past this control point, we have been able to investigate some of the transcriptional dynamics apparent before and after early egg formation over a 72 hour period. A short-day photoperiod (10:14 light:dark photoperiod) and moderate temperatures (11Â°C) are required for the induction of ovarian diapause. For our microarray experiments, 0-4 hours post-eclosion females were immediately introduced to the restrictive environmental conditions. Females were subsequently collected for RNA extraction after 5-7 days, or were alternatively re-introduced to normal environmental conditions to continue egg development. Time-points were collected before and after the release of reproductive arrest at 24 hour intervals and hybridized in a balanced loop design, including dye-swaps. Eight biological replicates were subsequently performed for each treatment, including 3 day old male and female controls maintained under normal environmental conditions.

2010-06-25 | E-GEOD-13303 | biostudies-arrayexpress

Global transcriptome analysis of orange wheat blossom midge, Sitodiplosis mosellana (Gehin) (Diptera: Cecidomyiidae) to identify candidate transcripts regulating diapause

Project description:Many insects enter a developmental arrest (diapause) that allows them to survive harsh seasonal conditions. Despite the well-established ecological significance of diapause, the molecular basis of this crucial adaptation remains largely unresolved. Sitodiplosis mosellana (Gehin), the orange wheat blossom midge (OWBM), causes serious damage to wheat throughout the northern hemisphere, and sporadic outbreaks occur in the world. Traits related to diapause appear to be important factors contributing to their rapid spread and outbreak. To better understand the diapause mechanisms of OWBM, we sequenced the transcriptome and determined the gene expression profile of this species.

2013-06-20 | GSE48156 | GEO

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