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Altered gene expression associated with thermo-sensitive male sterility in rapeseed (Brassica napus) mutant SP2S

ABSTRACT: To reveal the possible molecular mechanism underpinning the temperature sensitive male sterility, a comparative transcriptome analysis of the SP2S line and its NIL SP2F was conducted. Transcriptome differences between fertile and sterile plants were analyzed by using digital gene expression (DGE) tag profiling, and numerous differentially and specifically expressed transcripts were identified. By quantitative RT-PCR, expression levels of 10 genes randomly selected showed consistent expression patterns with the DGE data. A total of 827 up-regulated and 757 down-regulated expression transcripts in SP2S were identified, indicating the complex responses to temperature in the SP2S line. Some genes in important pathways including ubiquitin mediated proteolysis, phenylpropanoid biosynthesis, endocytosis, apoptosis, oxidative phosphorylation, purine metabolism, MAPK signaling, spliceosome, and ribosome were up-regulated in SP2S while other genes in the pathways of aminoacyl-tRNA biosynthesis, basal transcription factors, nucleotide excision repair, mismatch repair, and CO2 fixation were down-regulated. Some genes involved in photosynthesis, citrate cycle (TCA cycle), pentose phosphate, and stress-responsive were also differentially expressed.

ORGANISM(S): Brassica napus

PROVIDER: GSE69638 | GEO | 2017/01/01

SECONDARY ACCESSION(S): PRJNA286050

REPOSITORIES: GEO

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Project description:Effects of heat priming applied to the first generation on tolerance of the successive generation to post-anthesis high temperature stress were investigated. Compared with the progeny of non-heat primed plants (NH), the progeny of heat-primed plants (PH) presented higher grain yield, leaf photosynthesis and activities of antioxidant enzymes and lower cell membrane damage under high temperature stress. In the transcriptome profile, 1430 probes showed obvious difference in expression between PH and NH. These genes were those of signal transduction, transcription, energy, defense, and protein destination and storage, respectively. Pot experiments (25 cm in diameter and 22 cm in depth) were conducted at the Experimental Station of Nanjing Agricultural University, Nanjing, China (32Ë30Ê¹N and 118Ë42Ê¹E). During the first generation, wheat plants were divided into two groups: one group were not primed (N) while the other were heat-primed (P) at pre-anthesis (at a day/night temperature of 32/28ËC for two days at the seven-leaf stage and the nine-leaf stage) and post-anthesis (34/30ËC for seven days at the 10th day after anthesis). At maturity, seeds from both groups were harvested. Seedlings (the progeny generation) from each group seed were further divided two sub-groups: one was subjected to high temperature stress at a day/night temperature of 34/30ËC, while the other was set at 26/22ËC for six days from the 10th day after anthesis (DAA). During both priming and high-temperature stress, plants under different treatments were moved into sepatarate growth chambers at preset temperatures. Thereafter, four treatments were established: NC, progeny of non-primed plants without post-anthesis high temperature stress; NH, progeny of non-primed plants with post-anthesis high temperature stress; PC, progeny of primed plants without post-anthesis high temperature stress; and PH, progeny of primed plants with post-anthesis high temperature stress.

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Altered gene expression associated with thermo-sensitive male sterility in rapeseed (Brassica napus) mutant SP2S

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