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Analysis of wheat straw grown Trichoderma reesei gene expression as influenced by L-methionine in the medium

ABSTRACT: Wheat straw grown cultures of T. reesei QM9414 were supplemented with 100 µM L-methionine and the genome wide gene expression monitored in order to find novel L-Methionine repressible genes.

ORGANISM(S): Trichoderma reesei QM9414 Trichoderma reesei

PROVIDER: GSE69745 | GEO | 2015/06/11

SECONDARY ACCESSION(S): PRJNA286325

REPOSITORIES: GEO

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Analysis of wheat straw grown Trichoderma reesei gene expression as influenced by L-methionine in the medium

Project description:Wheat straw grown cultures of T. reesei QM9414 were supplemented with 100 µM L-methionine and the genome wide gene expression monitored in order to find novel L-Methionine repressible genes. Total RNA was isolated from independent duplicate shake flask cultures of T. reesei QM9414 pregrown on pretreated wheat straw. Global gene and analyzed using a 4 chip design where 2 chips each represented cultures with or without exogeneously added 100 µM L- Methionine.

2015-06-11 | E-GEOD-69745 | biostudies-arrayexpress

A G-protein coupled receptor supports methionine response in Trichoderma reesei

Project description:We investigated the function of the G-protein coupled receptor 72004 in Trichoderma reesei and found that it is involved in methionine response and gene expression in light and darkness

2024-01-01 | GSE222724 | GEO

Comparative analysis of the Trichoderma reesei transcriptome during growth on the cellulase inducing substrates wheat straw and lactose

Project description:We have examined and compared the transcriptome of T. reesei growing on wheat straw and lactose as carbon sources under otherwise similar conditions. Gene expression on wheat straw exceeded that on lactose, and 1619 genes were found to be only induced on wheat straw but not on lactose. They comprised 30 % of the CAZome, but were also enriched in genes associated with phospholipid metabolism, DNA synthesis and repair and iron homeostatis. Two thirds of the CAZome was expressed both on wheat straw as well as on lactose, but 60 % of it at least >2-fold higher on the former. Major wheat straw specific genes comprised xylanases, chitinases and M-CM-^_-mannosidases. Interestingly, the latter two CAZyme families were significantly higher expressed in a strain in which xyr1 encoding the major regulator of cellulase and hemicellulase biosynthesis is non-functional, demonstrating that XYR1 is a repressor of these genes. We used two biological replicas of four T. reesei strains growing on glucose, lactose, and on wheat straw

2013-09-17 | E-GEOD-46155 | biostudies-arrayexpress

Comparative analysis of the Trichoderma reesei transcriptome during growth on the cellulase inducing substrates wheat straw and lactose

Project description:We have examined and compared the transcriptome of T. reesei growing on wheat straw and lactose as carbon sources under otherwise similar conditions. Gene expression on wheat straw exceeded that on lactose, and 1619 genes were found to be only induced on wheat straw but not on lactose. They comprised 30 % of the CAZome, but were also enriched in genes associated with phospholipid metabolism, DNA synthesis and repair and iron homeostatis. Two thirds of the CAZome was expressed both on wheat straw as well as on lactose, but 60 % of it at least >2-fold higher on the former. Major wheat straw specific genes comprised xylanases, chitinases and ß-mannosidases. Interestingly, the latter two CAZyme families were significantly higher expressed in a strain in which xyr1 encoding the major regulator of cellulase and hemicellulase biosynthesis is non-functional, demonstrating that XYR1 is a repressor of these genes.

2013-09-17 | GSE46155 | GEO

ArrayCGH of Trichoderma reesei strains QM9123, QM9414, NG14 and RutC-30

Project description:Trichoderma reesei is the main industrial producer of cellulases and hemicellulases used to depolymerize biomass in many biotechnical applications. Many production strains in use have been generated by classical mutagenesis. In this study we characterized genomic alterations in hyperproducing mutants of T. reesei by high-resolution comparative genomic hybridisation tiling array. We carried out aCGH analysis of four hyperproducing strains (QM9123, QM9414, NG14 and RutC-30) using QM6a genome as a reference. ArrayCGH analysis identified dozens of mutations in each strain analyzed.

2010-11-26 | GSE19606 | GEO

Expression analysis of Trichoderma reesei QM9414 and delta-phlp1, delta-gnb1 and delta-gng1 in light and darkness.

Project description:Investigation of whole genome gene expression level changes in response to different light conditions of the T. reesei QM9414 parental strain and the deletion strains delta-phlp1, delta-gnb1 and delta gng1, cultivated on 1 % microcrystalline cellulose. The mutants analyzed in this study are further described in Tisch et al. 2011: Carbohydrate degradation is significantly regulated by light and the phosducin like protein PhLP1 in Trichoderma reesei (Hypocrea jecorina). We used two biological replicates of four T. reesei strains (QM9414, delta-phlp1, delta-gnb1 and delta-gng1), cultivated in constant light (LL, 1800 lux) or constant darkness (DD) on microcrystalline cellulose.

2011-03-02 | E-GEOD-27581 | biostudies-arrayexpress

Genome-wide transcriptional response of the filamentous fungus Trichoderma reesei to lignocellulose using RNA sequencing and comparison with Aspergillus niger

Project description:The induction of genes in response to exposure of T. reesei to wheat straw was explored using genome-wide RNA-seq and compared to published RNA-seq data and model of how A. niger senses and responds to the lignocellulose. After 24 h of exposure to straw, transcript levels of known and predicted lignocellulose-degrading enzymes increased to around 8% of total cellular mRNA in T. reesei, which was much less when compared to A. niger. The bulk of enzymes used to deconstruct wheat straw is similar in both fungi. Other, non-plant cell wall-degrading enzymes which may aid in lignocellulose degradation were also uncovered in T. reesei and similar to those described in A. niger. Antisense transcripts were also shown to be present in T. reesei and their expession can be regulated by the respective growth condition.

2013-04-01 | GSE44648 | GEO

Genome-wide transcriptional response of the filamentous fungus Trichoderma reesei to lignocellulose using RNA sequencing and comparison with Aspergillus niger

2013-04-01 | E-GEOD-44648 | biostudies-arrayexpress

The transcriptome of lae1 mutants of Trichoderma reesei cultivated at constant growth rates reveals new targets of LAE1 function

Project description:We used three biological replicas of T. reesei (QM9414), a Δlae1 mutant and a lae1-overexpressing strain in the chemostat on glucose at two different growth rates (0.075 and 0.020 h-1).

2014-03-07 | GSE55652 | GEO

Expression analysis of Trichoderma reesei QM9414 and delta-phlp1, delta-gnb1 and delta-gng1 in light and darkness.

2011-03-02 | GSE27581 | GEO

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