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Transcriptome profiling of the defense response following elicitation by a bacterial pathogen or flg22

ABSTRACT: Phytoalexins are abundant in edible crucifers and have important biological activities, yet no dedicated gene for their biosynthesis is known. Here, we report two new cytochromes P450 from the non-model plant Brassica rapa (Chinese cabbage) that catalyze unprecedented S-heterocyclizations in cyclobrassinin and spirobrassinin biosynthesis. Our results reveal the first genetic and biochemical insights into the biosynthesis of a prominent pair of dietary metabolites, and have important implications for pathway discovery across >20 recently sequenced non-model crucifers.

ORGANISM(S): Brassica rapa

PROVIDER: GSE69785 | GEO | 2015/09/21

SECONDARY ACCESSION(S): PRJNA286768

REPOSITORIES: GEO

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RNA-seq of Brassica rapa leaves elicited with either Pseudomonas syringae pv. maculicola or flg22

Project description:Phytoalexins are abundant in edible crucifers and have important biological activities, yet no dedicated gene for their biosynthesis is known. Here, we report two new cytochromes P450 from the non-model plant Brassica rapa (Chinese cabbage) that catalyze unprecedented S-heterocyclizations in cyclobrassinin and spirobrassinin biosynthesis. Our results reveal the first genetic and biochemical insights into the biosynthesis of a prominent pair of dietary metabolites, and have important implications for pathway discovery across >20 recently sequenced non-model crucifers. Leaf mRNA profiles of four conditions (Pseudomonas syringae pv. maculicola, flg22, and their respective mock treatments) were tested in triplicate.

2015-09-21 | E-GEOD-69785 | biostudies-arrayexpress

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iTRAQ-based quantitative proteomic analysis reveals new metabolic pathways responding to cold stress in two Winter Turnip Rape (Brassica rapa L.), part 2

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2019-11-12 | PXD008195 | Pride

iTRAQ-based quantitative proteomic analysis reveals new metabolic pathways responding to cold stress in two Winter Turnip Rape (Brassica rapa L.), part 1

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2014-10-10 | GSE46299 | GEO

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2012-03-30 | E-GEOD-28281 | biostudies-arrayexpress

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2010-05-19 | E-GEOD-15915 | biostudies-arrayexpress

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Project description:Deep sequencing provided evidence that a novel subset of small RNAs were derived from the chloroplast genome of Chinese cabbage (Brassica rapa) and Arabidopsis (Ler). The chloroplast small RNAs (csRNAs) include those derived from mRNA, rRNA, tRNA and intergenic RNA. The rRNA-derived csRNA were preferentially located at the 3â-ends of the rRNAs, while the tRNA-derived csRNAs were mainly located at 5â-termini of the tRNAs. After heat treatment, the abundance of csRNAs decreased in chinese cabbage seedlings, except those of 24 nt in length. The novel heat-responsive csRNAs and their locations in the chloroplast were verified by Northern blotting. The regulation of some csRNAs to the putative target genes were identified by real-time PCR. Our results indicated that high temperature regulated the production of some csRNAs, which may have potential roles in transcriptional or post-transcriptional regulation, and affected putative target genes expression in chloroplast.

2012-03-31 | GSE28281 | GEO

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