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Pseudomonas pseudoalcaligenes CECT5344 response to cyanide vs. ammonium or nitrogen starvation

ABSTRACT: Transcriptional analysis of the cyanotroph Pseudomonas pseudoalcaligenes CECT5344 in response to cyanide present in wastewaters from industrial activities, such as jewelry and electroplating activities

ORGANISM(S): Pseudomonas oleovorans CECT 5344

PROVIDER: GSE69930 | GEO | 2016/01/27

SECONDARY ACCESSION(S): PRJNA287143

REPOSITORIES: GEO

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Pseudomonas pseudoalcaligenes CECT5344 response to cyanide vs. ammonium or nitrogen starvation

Project description:Transcriptional analysis of the cyanotroph Pseudomonas pseudoalcaligenes CECT5344 in response to cyanide present in wastewaters from industrial activities, such as jewelry and electroplating activities Four-conditions experiment, including three different nitrogen sources (ammonium, sodium cyanide or a cyanide-containing wastewater). One experiment without nitrogen added to the media (nitrogen limited condition). Four biological replicates for each condition (added nitrogen source to the media) plus the experiment without nitrogen source added.

2016-01-27 | E-GEOD-69930 | biostudies-arrayexpress

Regulatory non-coding sRNA in response to cyanide in Pseudomonas pseudoalcaligenes CECT5344

Project description:Biological treatments to degrade cyanide are a powerful technology for cyanide removal from industrial wastewaters. It has been previously demonstrated that the alkaliphilic bacterium Pseudomonas pseudoalcaligenes CECT5344 is able to use free cyanide and several metal-cyanide complexes as the sole nitrogen source. In this work, the strain CECT5344 has been used for detoxification of the different chemical forms of cyanide that are present in alkaline wastewaters from the jewelry industry. This liquid residue also contains large concentrations of metals like iron, copper and zinc, making this wastewater even more toxic. To elucidate the molecular mechanisms involved in the bioremediation process, a quantitative sRNA sequencing analysis has been carried out in P. pseudoalcaligenes CECT5344 cells grown with the jewelry residue, free cyanide and ammonium as sole nitrogen sources.

2019-02-13 | GSE117374 | GEO

Quantitative proteomic analysis (LC-MS/MS) of Pseudomonas pseudoalcaligenes CECT5344 in response to industrial cyanide-containing wastewaters

Project description:Biological treatments to degrade cyanide have shown to be a powerful technology for cyanide removal from industrial wastewaters. It has been previously demonstrated that the alkaliphilic bacterium Pseudomonas pseudoalcaligenes CECT5344 is able to use free cyanide and several metal−cyanide complexes as the sole nitrogen source. The strain CECT5344 has been used for detoxification of the different chemical forms of cyanide that are present in alkaline wastewaters from the electroplating process of the jewelry industry. This liquid residue also contains large concentrations of metals like iron, copper and zinc, making this wastewater even more toxic. A proteomic analysis by LC-MS/MS has been applied to elucidate the molecular mechanisms involved in this bioremediation process in P. pseudoalcaligenes CECT5344. Among others, different proteins related to cyanide and cyanate assimilation, as well as other proteins involved in transport and resistance to metals were induced by the cyanide-containing jewelry residue have been identified.

2017-03-08 | PXD005056 | Pride

Comparative transcriptomics for growth of engineered Pseudomonas putida strains when grown using ammonium or nitrate nitrogen sources

Project description:We report the transcriptomic profiles of 2 different Pseudomonas putida derivatives when they are grown in MME medium supplemented with either ammonium or nitrate as the sole nitrogen source. Expression of both heterologous and native genes are examined.

2021-01-12 | GSE147420 | GEO

Cyanoalanine in Pseudomonas pseudoalcaligenes CECT5344

Project description:Quantitative proteomic analysis of Pseudomonas pseudoalcaligenes CECT5344 to compare the nitrogen metabolism when ammonium, beta-cyanoalanine and jewelry residue (with both free and metal-complexed cyanide) are used as sole nitrogen source

2022-02-17 | PXD026640 | Pride

Expression data from Pseudomonas aeruginosa PAO1 and an isogenic HCN negative mutant (PAO6344), grown under cyanogenic conditions.

Project description:Pseudomonas aeruginosa produces the toxic secondary metabolite hydrogen cyanide (HCN) at high cell population densities and low aeration. We have used Affimetrix microarrays to investigate the impact of HCN as a signal in cell-cell communication by comparing the transcriptome of the wild-type strain PAO1 to that of an HCN-negative mutant (PAO6344) under cyanogenic conditions.

2013-09-16 | GSE48587 | GEO

Iron-starvation effect on transcriptome of Pseudomonas fluorescens Pf-5

Project description:This SuperSeries is composed of the following subset Series: GSE29319: Iron-starvation effect on transcriptome of Pseudomonas fluorescens Pf-5: iron(II) chloride GSE29320: Iron-starvation effect on transcriptome of Pseudomonas fluorescens Pf-5: iron(III) chloride Refer to individual Series

2011-05-16 | E-GEOD-29322 | biostudies-arrayexpress

Bioelectrochemical nitrogen fixation to extracellular ammonium by <i>Pseudomonas stutzeri</i>.

Project description: Not available

| S-EPMC8090881 | biostudies-literature

Transcriptomic response of durum wheat to nitrogen starvation.

Project description: Not available

| S-EPMC5430780 | biostudies-literature

Inactivation of the GacA response regulator in Pseudomonas fluorescens Pf-5 has far-reaching transcriptomic consequences

Project description:The GacS/GacA signal transduction system is a central regulator in Pseudomonas spp., including the biological control strain P. fluorescens Pf-5, in which GacS/GacA controls the production of secondary metabolites and exoenzymes that suppress plant pathogens. A whole genome oligonucleotide microarray was developed for Pf-5 and used to assess the global transcriptomic consequences of a gacA mutation in P. fluorescens Pf-5. In cultures at the transition from exponential to stationary growth phase, GacA significantly influenced transcript levels of 632 genes, representing more than 10% of the 6147 annotated genes in the Pf-5 genome. Transcripts of genes involved in the production of hydrogen cyanide, the antibiotic pyoluteorin, and the extracellular protease AprA were at a low level in the gacA mutant, whereas those functioning in siderophore production and other aspects of iron homeostasis were significantly higher in the gacA mutant than in wild-type Pf-5. Notable effects of gacA inactivation were also observed in the transcription of genes encoding components of a type VI secretion system and cytochrome C oxidase subunits. Two novel gene clusters expressed under the control of gacA were identified from transcriptome analysis, and we propose global-regulator-based genome mining as an approach to decipher the secondary metabolome of Pseudomonas spp.

2010-03-04 | GSE16898 | GEO

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