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Small RNA-seq during the ecdysone and heat-shock response in Drosophila Kc167 cells

ABSTRACT: Small RNA sequencing libraries at 0 h, 3 h, and 48 h time points after ecdysone treatment and 20min heat shock (37 degrees celcius) in Drosophila cell culture

ORGANISM(S): Drosophila melanogaster

PROVIDER: GSE69934 | GEO | 2015/06/17

SECONDARY ACCESSION(S): PRJNA287161

REPOSITORIES: GEO

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Small RNA-seq during the ecdysone and heat-shock response in Drosophila Kc167 cells

Project description:Small RNA sequencing libraries at 0 h, 3 h, and 48 h time points after ecdysone treatment and 20min heat shock (37 degrees celcius) in Drosophila cell culture Examination of small RNA dynamics during differentiation

2015-06-17 | E-GEOD-69934 | biostudies-arrayexpress

ecdysone treated KC167 cells during Heat Shock and at Room Temperature

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Project description:HUVEC (Human Umbilical Vein Endothelial Cells) cells were exposed to heat shock (42 degrees Celcius) for different time points (1hr, 3 hrs, 6 hr and 12 hrs) and compared to non heat-shock cells. External normalization controls were added in equal amounts to equivalent amounts of total RNA.

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Heat-shock (Temp43, 30min, 60 min)

Project description:JN54 (wild-type) cells were incubated in YPD medium at 30 degrees C to a logarithmic phase (OD660=1), followed by treatment with mild heat-shock at 43 degrees C for 30 min in pre-warmed (43 degrees C) 100ml of YPD medium using 500 ml Erlenmeyer flask. JN54 (wild-type) cells were incubated in YPD medium at 30 degrees C to a logarithmic phase (OD660=1), followed by treatment with mild heat-shock at 43 degrees C for 60 min in pre-warmed (43 degrees C) 100ml of YPD medium using 500 ml Erlenmeyer flask. Keywords: Stress response

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Ecdysone- and stress-induced expression profile in Kc167 cells

Project description:Kc167 cells were mock-treated/treated with combinations of steroid hormone ecdysone and gamma-irradiation, and harvested. The expression profiles were determined using Affymetrix Drosophila Genechip 1 arrays. Comparisons between the sample groups allow the identification of genes with ecdysone- and/or radiation-responsive expression patterns. Keywords: response to hormone, stress response

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Embryo exposure to heat shock and teratogen

Project description:Analysis of embryos exposed to either: [1] 40 uM 4-hydroperoxycyclophosphamide for 1 or 5 hours at 37°C [2] 43°C heat shock for 15 minutes followed by 1 or 5 hours at 37°C This SuperSeries is composed of the SubSeries listed below.

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Transcription profiling of Drosophila Kc167 cells treaye with ecdysone and gamma irradiation to investigate ecdysone- and stress-induced expression profiles

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Heat shock - kin82 mutant and wildtype

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2003-04-15 | GSE406 | GEO

Embryo exposure to heat shock and teratogen

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