Dataset Information


Global gene expression profiling following a dietary selenium supplementation in sheep (Ovis aries).

ABSTRACT: In the present study, we studied the effect of dietary selenium (Se) supplementation on the transcriptomic profile of sheep. The main objective was to evaluate the effect of Se-supplementation on the overall transcriptome of sheep, the altered pathways, and the biological processes related to it . A custom oligo microarray platform (AMADID: 070119) was designed, then used to profile gene expression from 20 samples from 10 sheep at two time points (T0; before Se-supplementation, and T40; at the end of a 40-d Se-supplementation period). Isolated and purified total RNAs were individually hybridized to the custom (4x44k) DNA microarray. The comparison of control and treated animal transcriptomes revealed a large set of differentially expressed genes. After functional analysis and qPCR validation, the result showed several pathways and biological processes that have been altered following Se-supplementation to the diet. Overall design: Ten lactating crossbred ewes (3 to 4 y of age and 55 to 65 kg BW) were assigned to a before-and-after experimental design, where each animal served as its own control. The sheep have been acclimatized (4-wk) on a basal diet containing a maintenance level of Se (0.13 mg Se/kg DM). At the end of the 4-wk acclimatization period the sheep started to receive the supra Se-supplemented diet (0.45 mg Se/kg DM) for a 40-d period. Blood samples were collected at 2 time points [T0; one day before the supra Se-supplementation, and T40; at the end of the 40-d supra Se-supplementation phase]. Blood samples were collected from each individual animal directly into Paxgene blood collection tubes, processed and stored at -20˚C till further analyses. All samples yielded high quality RNA that was tested prior the microarray setup.

INSTRUMENT(S): Agilent-070119 Sheep Whole Transcriptome 4x44k.1 070000

SUBMITTER: Ramy Elgendy  

PROVIDER: GSE69997 | GEO | 2016-04-26



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