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Identification of mRNAs Regulated in Response to Transcriptional Activation of the Arabidopsis ABA INSENSITIVE GROWTH 1 (ABIG1) Transcription Factor cDNA

ABSTRACT: Liquid grown, seven day old wild type Arabidopsis seedlings and seedlings carrying a cDNA encoding the ABA INSENSITIVE GROWTH 1 (ABIG1) gene under the control of an estrogen inducible promoter were treated with estrogen for 0, 60 and 120 minutes at which point seedlings were flash frozen and mRNA was extracted.

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE70100 | GEO | 2016/06/22

SECONDARY ACCESSION(S): PRJNA287644

REPOSITORIES: GEO

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Identification of mRNAs Regulated in Response to Transcriptional Activation of the Arabidopsis Abscisic Acid Insensitive Growth 1 (AIG1) Transcription Factor cDNA

Project description:Liquid grown, seven day old wild type Arabidopsis seedlings and seedlings carrying a cDNA encoding the Abscisic Acid Insensitive Growth (AIG1) gene under the control of an estrogen inducible promoter were treated with estrogen for 0, 60 and 120 minutes at which point seedlings were flash frozen and mRNA was extracted. Wild-type and ESTAIG1 Col samples were treated with estrogen and flash frozen 0, 60 and 120 minutes later. Each sample type has three biological reps, except ESTHAT22.120, which has two. There are therefore 17 total samples. mRNA samples were sequenced by Otogenetics and then analyzed using the Tuxedo suite of applications.

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Drought treatment and ABA feeding to ABA insensitive Populus tremula × tremuloides lines

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SKIP regulates ABA-mediated alternative splicing and its signaling in Arabidopsis

Project description:Abscisic acid (ABA) plays a key role in plant development and responses to abiotic stress. A wide number of regulatory mechanisms on ABA perception and signaling are known, including transcriptional regulation and post-translational regulations; however, less to know about the post-transcriptional regulations. In this work, we have found that SKIP, a splicing factor in spliceosome, positively regulates ABA signal transduction. Mutation of SKIP, skip-1, confers ABA insensitive phenotype in seed germination, root growth, and ABA-responsive gene expression. Transformation of SKIP genomic DNA to skip-1 is able to recover its defects. SKIP binds to the pre-mRNA of genes in ABA signaling, such as PYL8, to regulate their splicing. The alternative splicing of PYL7, PYL8, ABF2, and ABI5 in ABA pathway is disrupted by skip-1, reducing the mRNA levels of them. The abnormal splicing of PP2Cs activates their transcription by skip-1, suggesting there is a feedback regulation for PP2Cs caused by skip-1. The down-regulation of PYL ABA receptors, ABF2 and ABI5 ABA response transcription factors, and the up-regulation of PP2Cs through alternative splicing reduce the plant responses to ABA in skip-1. SKIP is required for ABA-mediated genome-wide alternative splicing. ABA treatment enhances the novel splicing events in WT genome-widely. The novel alternative splicing events are increased by skip-1 in the absence and present of ABA. Our results first reveal a principle on how a splicing factor affects the ABA signaling.

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Expression data from Arabidopsis gapcp mutant treated with ABA

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