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Expression of AWR5 in wild type and cdc55 deletion mutant at 6 h of induction

ABSTRACT: Expressing the type III effector AWR5 of the phytopathogen Ralstonia solanacearum in yeast cells causes cessation of growth and transcriptional changes reminiscent of inhibition of the TOR pathway. Deletion of cdc55, a regulatory subunit of the PP2A Ser/Thr protein phosphatase eliminates the strong growth defect caused by AWR5 expression. This experiments aims to identify the impact of the cdc55 mutation in the transcriptome of AWR5-expressing cells.

ORGANISM(S): Saccharomyces cerevisiae

PROVIDER: GSE70331 | GEO | 2015/12/31

SECONDARY ACCESSION(S): PRJNA288324

REPOSITORIES: GEO

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Expression of AWR5 in wild type and cdc55 deletion mutant at 6 h of induction

Project description:Expressing the type III effector AWR5 of the phytopathogen Ralstonia solanacearum in yeast cells causes cessation of growth and transcriptional changes reminiscent of inhibition of the TOR pathway. Deletion of cdc55, a regulatory subunit of the PP2A Ser/Thr protein phosphatase eliminates the strong growth defect caused by AWR5 expression. This experiments aims to identify the impact of the cdc55 mutation in the transcriptome of AWR5-expressing cells. induction of AWR5 in JA-100 CDC55 tetO:AWR5-GFP and JA-100 cdc55 tetO:AWR5-GFP

2015-12-31 | E-GEOD-70331 | biostudies-arrayexpress

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Project description:Investigation of whole genome gene expression level changes in the bacterial wilt pathogen Ralstonia solanacearum, strain UW551 at 20°C and 28°C in culture and in planta. The temperatel strain UW551 can wilt and cause full-blown disease on tomato plants at 28°C as well as at 20°C. A 16 array study using total RNA recovered from the following: 8 separate cultures of Ralstonia solanacearum strain UW551 grown in rich medium-CPG (4 grown at 20°C and 4 grown at 28°C) 8 separate in planta samples of Ralstonia solanacearum strain UW551 harvested from diseased tomato plants cv. BonnyBest (4 recovered from plants grown at 20°C and 4 from plants grown at 28°C) Each array (4-plex format) measures the expression level of 4318 genes from Ralstonia solanacearum strain UW551 with 2 to 6, 40-70 mer probes per gene, with two-fold technical redundancy. The arrays also contain probes for intergenic regions with no technical replicates.

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Ralstonia solanacearum_GMI1000 and UW551_20 C and 28 C.

Project description:This SuperSeries is composed of the following subset Series: GSE33657: Expression analysis of Ralstonia solanacearum strain GMI1000 at 20°C and 28°C in rich medium (CPG) and in planta GSE33661: Expression analysis of Ralstonia solanacearum strain UW551 at 20°C and 28°C in rich medium (CPG) and in planta Refer to individual Series

2012-01-15 | E-GEOD-33662 | biostudies-arrayexpress

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