Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Microarray gene expression study of cycling vs arrested S. Pombe minimal cells

ABSTRACT: A minimal cell cycle control module is driving the cell cycle in these S. Pombe cells. During the experiment the cells are arrested or released to cycle through the different phases of the cell cycle.

ORGANISM(S): Schizosaccharomyces pombe Saccharomyces cerevisiae

PROVIDER: GSE70438 | GEO | 2016/04/01

SECONDARY ACCESSION(S): PRJNA289442

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Microarray gene expression study of cycling vs arrested S. Pombe minimal cells

Project description:A minimal cell cycle control module is driving the cell cycle in these S. Pombe cells. During the experiment the cells are arrested or released to cycle through the different phases of the cell cycle. Samples were collected at the indicated times points from arrested and cycling cells and expression of genes were measured by microarrays on an Affymetrix platform.

2016-04-01 | E-GEOD-70438 | biostudies-arrayexpress

RNAseq measurments of gene expression during simultanous replication and mitosis using the S. Pombe minimal cell cycle strain

Project description:Cell cycle arrested G1 cells were exposed to high Cdk1 activity, so undergo replication and mitosis at the same time. Samples were collected at the indicated time points (0, 20, 40) from released (R) vs. Arrested (A) cells.

2016-04-01 | E-GEOD-70435 | biostudies-arrayexpress

RNAseq measurments of gene expression during simultanous replication and mitosis using the S. Pombe minimal cell cycle strain

Project description:Cell cycle arrested G1 cells were exposed to high Cdk1 activity, so undergo replication and mitosis at the same time.

2016-04-01 | GSE70435 | GEO

Budding yeast cohesin (Mcd1) distribution in mitotically arrested cells

Project description:genomic localization of the budding yeast cohesin complex was mapped in mitotically arrested cells by Mcd1 ChIP followed by hybridization to high-density tiled microarrays Cells were synchronized first in G1 and then released into media containing the microtubule poison nocodazole. Cells were fixed and processed for ChIP once arrested as large-budded cells. Immunoprecipitated DNA and total genomic DNA not subjected to immunoprecipitation were competitively hybridized to Nimblegen whole genome arrays.

2009-09-30 | E-GEOD-18021 | biostudies-arrayexpress

Schizosaccharomyces pombe

Project description:Microarray gene expression study of cycling vs arrested S. Pombe minimal cells

| PRJNA289442 | ENA

Proteomic Analysis of the Response to Cell Cycle Arrests in Human Myeloid Leukemia Cells

Project description:Previously (Ly et al. 2014), we analyzed protein abundance changes across a â€˜minimally perturbedâ€™ cell cycle in human cells (NB4), using centrifugal elutriation to differentially enrich distinct cell cycle phases. Here, we compare data from elutriated cells with NB4 cells arrested at comparable phases using serum starvation, hydroxyurea, or RO-3306. While elutriated and arrested cells have similar patterns of DNA content and cyclin expression, a large fraction of the proteome changes detected in arrested cells are found to reflect arrest-specific responses (i.e., starvation, DNA damage, CDK1 inhibition), rather than physiological cell cycle regulation. For example, we show most cells arrested in G2 by CDK1 inhibition express abnormally high levels of replication and origin licensing factors, and are likely poised for genome re-replication. The protein data are available in the Encyclopedia of Proteome Dynamics (http://www.peptracker.com/epd/), an online, searchable resource.

2017-03-31 | MSV000080841 | MassIVE

Proteomic profiling of Palbociclib arrested cells ± APC/C inactivation.

Project description:Cellular proliferation is highly regulated to ensure proper tissue homeostasis and to prevent diseases such as cancer. In actively proliferating cells, entry into S phase of the cell cycle and initiation of DNA replication is promoted by inactivation of the E3 ubiquitin ligase APC/C (anaphase promoting complex/cyclosome), though the critical S phase-promoting substrates have not been fully elucidated. The APC/C is also active in cells that have exited the cell cycle and entered an arrested state, but whether APC/C activity is essential to maintain arrest is unclear. We found that APC/C inactivation is sufficient to bypass cellular arrest induced by the CDK4/6 inhibitor Palbociclib. To identify potential APC/C substrates responsible for driving the escape from cell cycle arrest, we arrested cells using Palbociclib and then induced EMI1, inactivating the APC/C. Samples were collected at 0, 8, 16 and 20h after arrest and analyzed using proteomics. We found that inactivation of the APC/C promotes cell cycle re-entry by inducing RB phosphorylation and E2F-dependent gene transcription. Stabilization of both cyclin A and cyclin B contributes to arrest bypass, but only cyclin A accumulation is absolutely required. Direct expression of APC/C-resistant cyclin A, but not cyclin B, in arrested cells induces S phase entry analogous to APC/C inactivation. Cells bypassing arrest initiate DNA replication with severely reduced origin licensing, at least in part due to premature geminin accumulation. As a result, cells exhibit reduced rates of DNA synthesis, which leads to the accumulation of replication stress and long-term proliferation defects. Our findings suggest that CDK4/6 inhibition in cancers with reduced APC/C activity may be ineffective at promoting cytostatic cell cycle arrest but may nonetheless lead to elevated levels of replication stress that ultimately leads to a more durable cell cycle withdrawal.

2024-07-18 | PXD046783 | Pride

Investigating global transcript dynamics in mitotically arrested budding yeast cells

Project description:Transcript dynamics in mitotic exit mutants in the S. cerevisiae BF264-15D strain background. We examined the extent to which periodic cell-cycle transcription persisted in cells arrested in anaphase with intermediate level of B-cyclins.

2017-09-22 | GSE96997 | GEO

Proteomic Analysis of the Response to Cell Cycle Arrests in Human Myeloid Leukemia Cells

2015-07-23 | PXD001610 | Pride

Single-cell whole-genome sequencing of aneuploid cycling and aneuploid arrested cells

Project description:Single-cell whole-genome sequencing of aneuploid cycling and aneuploid arrested cells

| PRJEB58407 | ENA