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Transcriptomic profiling of S. cerevisiae wild type and aft2 mutant in response to exposure to selenite

ABSTRACT: The yeast S. cerevisiae does not require selenium for growth, so it is an excellent model to investigate the toxicity of this element without interference from its requirement as growth factor as occurs in animal cells. Exposure to selenite interferes with the yeast iron metabolism. Aft2 is a transcription factor related (with Aft1) to maintenance of iron homeostasis. We have found that aft2 cells are highly sensitive to selenite (a common environmental source for selenium). In these experiments we investigate the transcriptional response to a sub-lethal dose of selenite in wild type and aft2 cells. Our results show that the aft2 mutation strongly potentiates the transcriptional response to selenite, showing induction of many genes related to the responses to oxidative stress and DNA damage.

ORGANISM(S): Saccharomyces cerevisiae

PROVIDER: GSE70835 | GEO | 2015/12/31

SECONDARY ACCESSION(S): PRJNA289663

REPOSITORIES: GEO

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Transcriptomic profiling of S. cerevisiae wild type and aft2 mutant in response to exposure to selenite

Project description:The yeast S. cerevisiae does not require selenium for growth, so it is an excellent model to investigate the toxicity of this element without interference from its requirement as growth factor as occurs in animal cells. Exposure to selenite interferes with the yeast iron metabolism. Aft2 is a transcription factor related (with Aft1) to maintenance of iron homeostasis. We have found that aft2 cells are highly sensitive to selenite (a common environmental source for selenium). In these experiments we investigate the transcriptional response to a sub-lethal dose of selenite in wild type and aft2 cells. Our results show that the aft2 mutation strongly potentiates the transcriptional response to selenite, showing induction of many genes related to the responses to oxidative stress and DNA damage. Wild type (W303-1A) background and its MML1086 derivative (Daft2) were exposed to 1 mm selenite and samples taken at time 0, 1, 3 and 5 h of selenite treatment. Two biological replicates were prepared for each time-point. Therefore, there are 8 samples per replicate (4 samples for WT and 4 samples for aft2 cells). The WT strain is used here as reference.

2015-12-31 | E-GEOD-70835 | biostudies-arrayexpress

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Project description:Iron homeostasis in the yeast Saccharomyces cerevisiae is regulated at the transcriptional level by Aft1p, which activates the expression of its target genes in response to low-iron conditions. The yeast genome contains a paralog of AFT1, which has been designated AFT2. To establish whether AFT1 and AFT2 have overlapping functions, a mutant containing a double aft1aft2 deletion was generated. Growth assays established that the single aft2 strain exhibited no iron-dependent phenotype. However, the double-mutant aft1aft2 strain was more sensitive to low-iron growth conditions than the single-mutant aft1 strain. A mutant allele of AFT2 (AFT2-1up), or overexpression of the wild-type AFT2 gene, led to partial complementation of the respiratory- deficient phenotype of the aft1 strain. The AFT2-1up allele also increased the uptake of 59Fe in an aft1 strain. DNA microarrays were used to identify genes regulated by AFT2. Some of the AFT2-regulated genes are known to be regulated by Aft1p; however, AFT2-1up-dependent activation was independent of Aft1p. The kinetics of induction of two genes activated by the AFT2-1up allele are consistent with Aft2p acting as a direct transcriptional factor. Truncated forms of Aft1p and Aft2p bound to a DNA duplex containing the Aft1p binding site in vitro. The wild-type allele of AFT2 activated transcription in response to growth under low-iron conditions. Together, these data suggest that yeast has a second regulatory pathway for the iron regulon, with AFT1 and AFT2 playing partially redundant roles. Set of arrays organized by shared biological context, such as organism, tumors types, processes, etc. Keywords: Logical Set

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