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Comparative expression analysis of BRCA1mosMe fibroblast vs. BRCA1wt fibroblasts

ABSTRACT: We performed a comprehensive molecular and cellular analysis of primary dermal fibroblasts taken from a patient with recurrent cancers, harboring a BRCA1 mosaic epimutation (BRCA1mosMe) in comparison to their isogenic control fibroblasts (BRCA1wt), taken from the patient´s healthy monozygous sister.

ORGANISM(S): Homo sapiens

PROVIDER: GSE71078 | GEO | 2015/12/31

SECONDARY ACCESSION(S): PRJNA290317

REPOSITORIES: GEO

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Comparative expression analysis of BRCA1mosMe fibroblast vs. BRCA1wt fibroblasts

Project description:We performed a comprehensive molecular and cellular analysis of primary dermal fibroblasts taken from a patient with recurrent cancers, harboring a BRCA1 mosaic epimutation (BRCA1mosMe) in comparison to their isogenic control fibroblasts (BRCA1wt), taken from the patientÂ´s healthy monozygous sister. 4 independent samples per individual

2015-12-31 | E-GEOD-71078 | biostudies-arrayexpress

Epidermolysis bullosa associated cutaneous SCC fibroblast expression profiling

Project description:Patients with the genetic skin blistering disease recessive dystrophic epidermolysis bullosa (RDEB) develop aggressive cutaneous squamous cell carcinoma (cSCC). Metastasis leading to mortality is greater in RDEB than in other patient groups with cSCC. Here we investigate the dermal component in RDEB using mRNA expression profiling to compare cultured fibroblasts isolated from individuals without cSCC and directly from tumor matrix in RDEB and non-RDEB samples. While gene expression of RDEB normal skin fibroblasts resembled that of cancer-associated fibroblasts, RDEB cancer-associated fibroblasts exhibited a distinct and divergent gene expression profile, with a large proportion of the differentially expressed genes involved in matrix and cell adhesion. RDEB cancer-associated fibroblasts conferred increased adhesion and invasion to tumor and non-tumor keratinocytes. Reduction of COL7A1, the defective gene in RDEB, in normal dermal fibroblasts led to increased type XII collagen, thrombospondin-1 and Wnt-5A, while re-expression of wild type COL7A1 in RDEB fibroblasts decreased type XII collagen, thrombospondin- 1, and Wnt-5A expression, reduced tumor cell invasion in organotypic culture, and restricted tumor growth in vivo. Overall our findings demonstrate that matrix composition in patients with RDEB is a permissive environment for tumor development, and type VII collagen directly regulates the composition of matrix proteins secreted by dermal and cancer-associated fibroblasts. 16 samples

2012-05-15 | E-GEOD-37738 | biostudies-arrayexpress

Epidermolysis bullosa associated cutaneous SCC fibroblast expression profiling

2012-05-16 | GSE37738 | GEO

In vitro Exon Level Expression Profiling of dermal fibroblast

Project description:Transcriptome analysis of of control inhibitor and miR200b inhibitor transfected Human Dermal Adult Fibroblasts (HDAF) compared with Human Dermal Microvascular Endothelial Cells (HMEC). Injury induced inhibition of miR200b induces angiogenesis at the wound edges which help in the healing process. We have characterised the effect of miR200b suppression in Human Adult Dermal Fibroblasts converts to endothelial cells through transcriptional profiling. In this dataset, we include the expression data obtained from control inhibitor and miR200b inhibitor transfected Human Dermal Adult Fibroblasts, as well as Human Dermal Microvascular Endothelial Cells (HMEC) as positive control.

2017-12-31 | GSE89715 | GEO

Weige_450k_Control

Project description:Control human dermal fibroblasts from patient forearm

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Weige_450k_Case

Project description:Case human dermal fibroblasts from patient forearm

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Microarray analyses of fibroblast cell lines derived from a pain insensitive individual and 4 gender-matched controls

Project description:We have identified a pain insensitive individual carrying a microdeletion in the FAAH-OUT gene and a hypomorphic SNP in FAAH. A punch skin biopsy was taken from the individual and 4 gender matched controls and primary cultures of dermal fibroblasts were passaged. Total RNA was isolated from each cell line and analysed using microarrays to identify dysregulated genes.

2023-05-01 | E-MTAB-11809 | biostudies-arrayexpress

Single-cell transcriptomes of the aging human skin reveal loss of fibroblast priming

Project description:Fibroblasts are the main dermal cell type and are essential for the architecture and function of human skin. Important differences have been described between fibroblasts localized in distinct dermal layers, and these cells are also known to perform varied functions. However, this phenomenon has not been analyzed comprehensively yet. Here we have used single-cell RNA sequencing to analyze >15,000 cells from a sun-protected area in young and old donors. Our results define four main fibroblast subpopulations that can be spatially localized and functionally distinguished. Importantly, intrinsic aging reduces this fibroblast ‘priming’, generates distinct expression patterns of skin aging-associated genes, and substantially reduces the interactions of dermal fibroblasts with other skin cell types. Our work thus provides comprehensive evidence for a functional specialization of human dermal fibroblasts and suggests that the age-related loss of fibroblast priming contributes to human skin aging.

2020-03-31 | GSE130973 | GEO

MFAP5 regulates skin scar formation and fibroblast activation

Project description:A subset of human skin fibroblasts are phagocytic. To investigate the gene expression changes between phagocytosing and non-phagocytosing fibroblasts, fibroblasts were incubated with apoptotic human dermal microvascular endothelial cells expressing green fluorescent protein or no treatment. Treated fibroblasts were then collected after 8 hours and sorted by FACS to identify phagocytic and non-phagocytic fibroblasts. We then performed gene expression profiling analysis using data obtained from RNA-seq of these three groups of cells.

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Effect of CDDO-2P-Im Treatment in Fibroblast Cells

Project description:Primary Dermal Fibroblasts (PDF) were treated with various concentrations of CDDO-2P-Im for 6 hours and then RNA was extracted for gene expression changes

2023-04-20 | GSE229704 | GEO

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