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Mesenchymal stem cells use extracellular vesicles to outsource mitophagy and shuttle micro RNAs

ABSTRACT: Background: Mesenchymal stem cells (MSCs) and macrophages are fundamental components of the stem cell niche and function coordinately to regulate hematopoietic stem cell self-renewal and mobilization. Recent studies indicate that mitophagy and healthy mitochondrial function are critical to the survival of stem cells but how these processes are regulated in MSCs is unknown. Here we show that MSCs manage intracellular oxidative stress by targeting depolarized mitochondria to the plasma membrane via arrestin domain-containing protein 1-mediated microvesicles , which are then engulfed and re-utilized via a process involving fusion by macrophages resulting in enhanced bio-energetics. Furthermore, we show that MSCs simultaneously shed miRNA-containing exosomes that inhibit macrophage activation by suppressing Toll-like receptor (TLR) signaling, thereby de-sensitizing macrophages to the ingested mitochondria. Collectively, these studies mechanistically link mitophagy and MSC survival with macrophage function, thereby providing a physiologically relevant context for the innate immuno-modulatory activity of MSCs.

ORGANISM(S): Homo sapiens

PROVIDER: GSE71241 | GEO | 2015/08/17

SECONDARY ACCESSION(S): PRJNA290656

REPOSITORIES: GEO

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