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Spatial reorganization of telomeres in long-lived quiescent cells

ABSTRACT: Two conditions experiment, ChIP-chip anti-Sir3 comparing Sir3 occupancy in exponentially growing S.cerevisiae cells to Sir3 occupancy in the dense fraction of 7 days YPD culture of S.cerevisiae

ORGANISM(S): Saccharomyces cerevisiae

PROVIDER: GSE71273 | GEO | 2015/08/27

SECONDARY ACCESSION(S): PRJNA290776

REPOSITORIES: GEO

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Spatial reorganization of telomeres in long-lived quiescent cells

Project description:Two conditions experiment, ChIP-chip anti-Sir3 comparing Sir3 occupancy in exponentially growing S.cerevisiae cells to Sir3 occupancy in the dense fraction of 7 days YPD culture of S.cerevisiae Two conditions experiment with biological duplicates. Both conditions were processed on the same array, duplicates have been carried separately

2015-08-27 | E-GEOD-71273 | biostudies-arrayexpress

Recombination at subtelomeres is regulated by physical distance, double strand break resection and chromatin status

Project description:Two conditions experiment, ChIP-chip anti-Sir3 comparing Sir3 occupancy in exponentially growing S.cerevisiae cells to Sir3 occupancy in strains overexpressing SIR3

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Spatial reorganization of telomeres in long-lived quiescent cells.

Project description: Not available

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Analysis of a novel existence region of Sir3p in S. cerevisiae

Project description:In the budding yeast, HMR, HML, telomere and rDNA domain are known as a silencing region. Sir2 need to make it at rDNA and, HMR, HML and the telomere need to Sir2, Sir3, Sir4 complex to control internal gene repression. In this report, we found a newly Sir3 binding domain, CN domain (Chromosome New region) 1~14, by the ChIP on chip analysis on S.cerevisiae chromosome. In addition, we also performed ChIP on chip analysis with anti-Sir3 antibody using G1 phase synchronized cell to find Sir3 distribution difference of stage of cell cycle and we found CN15~CN25 which was G1 phase specific Sir3 binding region. Furthermore, we analyzed difference of gene expression at CN region in sir3 strain, and some regions did not change level of gene expression. In the conventional report, Sir3 had recruited by Sir2 and Sir4 on chromosome, but recruit of Sir3 was independent on Sir2 and Sir4 at some CN regions. These data suggested that we found a newly Sir3 function and Sir3 recruited system on chromosome.

2015-11-18 | GSE75075 | GEO

Translatome and transcriptome analysis of TMA20 (MCT-1) and TMA64 (eIF2D) knockout yeast strains

Project description:We report ribosome profiling and RNA-Seq data of S.cerevisiae strains with TMA20, TMA64 single- and TMA20/TMA64 double-gene knockouts, grown in the rich YPD or minimal SD medium.

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Transcriptional effects of the IWR1 deletion

Project description:Analysis of the genome-wide transcrptional effects caused by the deletion of the IWR1 gene by comparing the transcriptional profile of a iwr1 mutant strain with the isogenic wild type strain when cells were exponentially grown in YPD medium.

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Genomic Run-On (GRO) experiment for the YPD to YPGal time course

Project description:Timecourse analyses (0 to 850 min) of exponentially growing BQS252 yeast after shift from YPD to YPGal galactose medium. Total RNA in vivo labeled by run-on, or cDNA labelling using random decamers included. Genomic DNA also examined. Keywords: other

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The expression profile under cooper sulfate treatment

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Caloric restriction causes a distinct reorganization of the lipidome in quiescent and non-quiescent cells of budding yeast.

Project description: Not available

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Gene expression of subpopulations arising from a nitrogen down-shift with proline as a nitrogen source in S. cerevisiae

Project description:When S. cerevisiae cells growing exponentially in rich media (YPD) are shifted to nitrogen-limited media with proline (0.8 mM) as a nitrogen source, they differentiate into two subpopulations of different phenotypes including cell size, growth and survival capabilities. The RNA-seq data presented here are for each subpopulation (5 biological replicates per subpopulation) sampled 1 hour after the shift and separated by FACS.

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