Transcriptomics,Genomics

Dataset Information

165

A mechanism for expansion of the regulatory T cell repertoire and its role in enforcing self-tolerance.


ABSTRACT: Thymic Treg cells, mature non-Treg CD4+ single positive thymocytes, peripheral (spleen) resting and activated Treg cells were sorted from Foxp3-gfp reporter (wid type, WT) mice or Foxp3 enhancer CNS3 knockout (KO, carrying the same GFP reporter) mice. Total RNA was extracted and used for RNA sequencing to assess gene expression profiles. Overall design: Two 6-8 week old littermates of male Foxp3-gfp and Foxp3ΔCNS3-gfp mice were used to sort Treg cells and conventional CD4+ T cells. Lymphocyte preparation and electronic sorting were performed at the same time. RNA extraction, SMART amplification, library preparation were conducted in parallel.

INSTRUMENT(S): Illumina HiSeq 2000 (Mus musculus)

SUBMITTER: Hatice Osmanbeyoglu 

PROVIDER: GSE71309 | GEO | 2015-10-01

SECONDARY ACCESSION(S): PRJNA290884

REPOSITORIES: GEO

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Publications


T-cell receptor (TCR) signalling has a key role in determining T-cell fate. Precursor cells expressing TCRs within a certain low-affinity range for complexes of self-peptide and major histocompatibility complex (MHC) undergo positive selection and differentiate into naive T cells expressing a highly diverse self-MHC-restricted TCR repertoire. In contrast, precursors displaying TCRs with a high affinity for 'self' are either eliminated through TCR-agonist-induced apoptosis (negative selection) or  ...[more]

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