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Digital proteomics by DNA sequencing


ABSTRACT: The simultaneous measurement of many different proteins presents a major challenge to the fields of surgical and clinical pathology, and there is a genuine need for the development of a more direct, quantitative approach to multiplex analysis of proteins in tissues and clinical assays. Here we present a novel, high-throughput immunoassay, driven by the hypothesis that highly sensitive, multiplex protein characterization can be obtained by coupling the unique specificity of antibodies with massively parallel sequencing to detect and quantitate antigens from formalin-fixed, paraffin-embedded tissue (FFPE) and in solution. The method involves conjugating antibodies with a universal anchor oligonucleotide that is hybridized with a second oligonucleotide specifying the sample, antibody, and a unique molecule identifier, akin to “make, model and serial” number. Recovery of the data-rich oligonucleotide from mixtures of antibodies from samples such as histological tissue sections, (herein breast carcinoma) or extracts in an ELISA-type format, enabling error free-amplification that increases the sensitivity and expands the range of analysis.

ORGANISM(S): synthetic construct Homo sapiens

PROVIDER: GSE71486 | GEO | 2025/07/01

REPOSITORIES: GEO

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