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Time course of the mRNA expression in wild-type Thermus thermophilus HB8 strain grown on a rich medium (labeling:ENZO)

ABSTRACT: We observed the expression profile of the total mRNA of wild-type Thermus thermophilus HB8 strain grown in a rich (TT) medium at 70oC. Keywords: time course

ORGANISM(S): Thermus thermophilus HB8

PROVIDER: GSE7165 | GEO | 2007/08/05

SECONDARY ACCESSION(S): PRJNA104053

REPOSITORIES: GEO

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Time course of the mRNA expression in wild-type Thermus thermophilus HB8 strain grown on a rich medium (labeling:ENZO)

Project description:We observed the expression profile of the total mRNA of wild-type Thermus thermophilus HB8 strain grown in a rich (TT) medium at 70oC. Keywords: time course Three wild-type T. thermophilus HB8 strains were pre-cultured at 70oC for 16 h in 3 ml of TT medium containing 0.8% polypeptone, 0.4% yeast extract, 0.2% NaCl, 0.4 mM CaCl2, and 0.4 mM MgCl2, which was adjusted to pH 7.2 with NaOH. The cells (2 ml) were inoculated into 1 liter of the same medium and then cultivated at 70oC. Cells were collected after 180, 240, 300, 360, 420, 480, 540, 600, 680, and 760 min, and then crude RNA was extracted. The expression of each mRNA at each time point was analyzed on a GeneChip as described under Sample Description Sheet of each sample in this website.

2010-05-27 | E-GEOD-7165 | biostudies-arrayexpress

mRNA expression in ttcsp1 deletion mutant and wild type Thermus thermophilus HB8 strains grown on a rich medium

Project description:We observed the expression profile of the total mRNA of ttcsp1 deletion mutant of Thermus thermophilus HB8 strain grown in a rich (TR) medium at 45oC compared with that of 70oC. Keywords: temperature comparison

2009-07-31 | GSE12436 | GEO

Expression profile of crp-deficient Thermus thermophilus HB8 strain

Project description:We observed the expression profile of the total mRNA of crp (TTHA1437) deletion mutant of Thermus thermophilus HB8 strain grown in a rich (TT) medium at 70 degC

2010-10-15 | GSE21459 | GEO

Comparative expression analysis between CRP (TTHA1437) deletion mutant and wild-type of T. thermophilus HB8

Project description:We observed the expression profile of the total mRNA of crp (TTHA1437) deletion mutant of Thermus thermophilus HB8 strain grown in a rich (TT) medium at 70oC compared with that of wild type. Keywords: cell type comparison

2007-08-05 | GSE7166 | GEO

Comparative expression analysis between sigE (TTHB211) deletion mutant and wild-type of T. thermophilus HB8

Project description:We observed the expression profile of the total mRNA of sigE (TTHB211) deletion mutant of Thermus thermophilus HB8 strain grown in a rich (TT) medium at 70oC compared with that of wild type. Keywords: cell type comparison

2007-09-04 | GSE8780 | GEO

mRNA expression in wild-type of Thermus thermophilus HB8 strain grown on a rich (TT) medium at 45˚C and 70˚C

Project description:We observed the expression profile of the total mRNA of wild-type of Thermus thermophilus HB8 strain grown in a rich (TT) medium at 45˚C compared with that of 70˚C. Keywords: Growth temperature

2010-08-23 | GSE22962 | GEO

Comparative expression analysis between TTHA0101-deficient mutant and wild-type of Thermus thermophilus HB8

Project description:We observed the expression profile of the total mRNA of TTHA0101 deletion mutant of Thermus thermophilus HB8 strain grown in a rich (TT) medium at 70°C compared with that of wild type.

2010-10-15 | GSE24184 | GEO

mRNA expression in wild type and TTHA0175-deficient Thermus thermophilus HB8 strains; rich medium with growth temperature shift from 70C to 45C

Project description:In order to find function of TTHA0175 protein, we analyzed altered expression profile of the total mRNA of TTHA0175-deficient Thermus thermophilus HB8 strain grown in a rich (TT) medium after shifted down the growth temperature from 70 oC to 45 oC, and compared to that in the cases of wild type. Wild-type and TTHA0175-deficient T. thermophilus HB8 strains were each pre-cultured at 70oC for 16 h in 3 ml of TT medium containing 0.8% polypeptone, 0.4% yeast extract, 0.2% NaCl, 0.4 mM CaCl2, and 0.4 mM MgCl2, which was adjusted to pH 7.2 with NaOH. The cells (0.3 ml) were inoculated into 0.15 liter of the same medium and then cultivated at 70oC. At the Abs600=0.8 (0min), cold medium was added and the temperature were shifted down to 45oC. Cells were collected at 0, 30, and 120 min after temperature shift, and then crude RNA was extracted. In order to compare mRNA expression between 45oC and 70oC both in the cases of wild-type and TTHA0175-deficient strains, the expression of each mRNA at each time point was analyzed on a GeneChip.

2010-10-15 | E-GEOD-21195 | biostudies-arrayexpress

Comparative expression analysis between PfmR (TTHB023) deletion mutant and wild-type of Thermus thermophilus HB8

Project description:We observed the expression profile of the total mRNA of PfmR (TTHB023) deletion mutant of Thermus thermophilus HB8 strain grown in a rich (TT) medium at 70°C compared with that of wild type.

2013-03-18 | GSE36909 | GEO

Transcription profiling of Thermus thermophilus HB8 wild-type strain grown on a rich medium

Project description:We observed the expression profile of the total mRNA of wild-type Thermus thermophilus HB8 strain grown in a rich (TR) medium at 45oC compared with that of 70oC. Experiment Overall Design: wild-type T. thermophilus HB8 strains were each pre-cultured at 70oC for 16 h in 3 ml of TR medium containing 0.8% polypeptone, 0.4% yeast extract, 0.2% NaCl, 0.4 mM CaCl2, and 0.4 mM MgCl2, which was adjusted to pH 7.2 with NaOH. The cells (0.3 ml) were inoculated into 0.15 liter of the same medium and then cultivated at 70oC. At the Abs600=0.8 (0min), cold medium was added and the temperature were shifted down to 45oC. Cells were collected at 0min and 30min after temperature shift, and then crude RNA was extracted. In order to compare mRNA expression in thewild-type cell between 45oC and 70oC, the expression of each mRNA at each time point was analyzed on a GeneChip as described under Sample Description Sheet of each sample in this website.

2009-01-09 | E-GEOD-12437 | biostudies-arrayexpress

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