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Immortalized human hepatocyte (IHH) microRNA response to interferon alpha treatment

ABSTRACT: The purpose of this experiment was to obtain samples for microRNA analysis in IHH cells infected with human interferon alpha.

ORGANISM(S): Homo sapiens

PROVIDER: GSE71733 | GEO | 2015/08/06

SECONDARY ACCESSION(S): PRJNA292000

REPOSITORIES: GEO

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Immortalized human hepatocyte (IHH) microRNA response to interferon alpha treatment

Project description:The purpose of this experiment was to obtain samples for microRNA analysis in IHH cells infected with human interferon alpha. Immortalized human hepatocyte (IHH) cells were seeded at 1 x 10^6 cells per well on 6 well plates 3 days before infection. Treatments were initiated by replacing culture medium (containing 10% FBS) with fresh medium containing 500 U/ml of human interferon α (PBL Biosciences). Mock-treated cells were given normal culture medium only. Mock and treated cells were incubated at 37°C until sample collection time points. Infected samples were collected in triplet; time-matched mocks were collected in triplet in parallel with infected samples. Time points: 6, 12, and 18 h post-infection

2015-08-06 | E-GEOD-71733 | biostudies-arrayexpress

Immortalized human hepatocyte (IHH) mRNA response to interferon alpha treatment

Project description:The purpose of this experiment was to obtain samples for mRNA analysis in IHH cells infected with human interferon alpha.

2015-08-06 | GSE71732 | GEO

Immortalized human hepatocyte (IHH) mRNA response to interferon alpha treatment

Project description:The purpose of this experiment was to obtain samples for mRNA analysis in IHH cells infected with human interferon alpha. Immortalized human hepatocyte (IHH) cells were seeded at 1 x 10^6 cells per well on 6 well plates 3 days before infection. Treatments were initiated by replacing culture medium (containing 10% FBS) with fresh medium containing 500 U/ml of human interferon α (PBL Biosciences). Mock-treated cells were given normal culture medium only. Mock and treated cells were incubated at 37°C until sample collection time points. Infected samples were collected in triplet; time-matched mocks were collected in triplet in parallel with infected samples. Time points: 6, 12, and 18 h post-infection

2015-08-06 | E-GEOD-71732 | biostudies-arrayexpress

Immortalized Human Hepatocyte (IHH) transcriptome response to wild-type Ebola viruses Zaire Ebola (ZEBOV '76) and Reston Ebola (REBOV '08)

Project description:The purpose of this experiment was to obtain samples for transcriptome analysis using wild-type viruses: Zaire Ebola (ZEBOV '76) and Reston Ebola (REBOV '08). Overview of Experiment: Cells: Immortalized Human Hepatocytes (IHH); seed 60,000 cells per well in a 24-well plate. Infected with a multiplicity of infection (MOI) of 0.5. After infection, 3x wash with PBS and replace with 5% FCS DMEM without NaPyr or NEAA. Time matched mocks done in triplicate from same cell stock as rest of samples. Time Points = 0, 8, 24, 48, and 72 hrs post infection in triplicate. (For miRNA: 8, 24, 48, 72 hours.)

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Peroxisomal abnormalities in the immortalized human hepatocyte (IHH) cell line.

Project description: Not available

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IHH microRNA response to genetically-reconstructed Zaire Ebola viruses [miRNA]

Project description:The purpose of this experiment was to obtain samples for mRNA analysis in IHH cells infected with Zaire Ebola virus and mutants: Zaire Ebola virus: This wild-type Ebola virus - strain Mayinga - was isolated from a fatal human case in Zaire (now known as the Democratic Republic of Congo) in 1976 Zaire Ebola virus, VP35 R312A possesses a R312A mutation in the VP35 protein. Zaire Ebola virus, delta sGP. Lacks the ability to produce non-structural protein, the secreted glycoprotein (sGP). Zaire Ebola virus, delta mucin. Lacks the mucin-like domain (MLD), which contains both N-linked and O-linked glycosylation sites, for the glycoproteins. Overview of Experiment: Cells: Immortalized Human Hepatocytes (IHH); seed 60,000 cells per well in a 24-well plate. Infected with a multiplicity of infection (MOI) of 0.5. After infection, 3x wash with PBS and replace with 5% FCS DMEM without NaPyr or NEAA. Time matched mocks done in triplicate from same cell stock as rest of samples. Time Points = 0, 6, 12, 24, 48, and 72 hrs post infection in triplicate.

2015-06-18 | E-GEOD-69943 | biostudies-arrayexpress

IHH microRNA response to genetically-reconstructed Zaire Ebola viruses [miRNA]

2015-06-18 | GSE69943 | GEO

Transcriptomes comparisons of Dhh KO, Ihh KO and Dhh/Ihh DKO ovaries

Project description:The goal of the microarray analysis is to determine the redundant and distinct roles of Dhh and Ihh in ovarian functions

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Primary human lung microvascular endothelial cells treated with universal interferon alpha beta or interferon gamma

Project description:The purpose is to obtain samples for mRNA analysis in primary human lung microvascular endothelial cells treated with universal interferon alpha beta or interferon gamma.

2017-12-27 | GSE106524 | GEO

Immortalized Human Hepatocyte (IHH) transcriptome response to wild-type Ebola viruses Zaire Ebola (ZEBOV '76) and Reston Ebola (REBOV '08)

Project description:The purpose of this experiment was to obtain samples for transcriptome analysis using wild-type viruses: Zaire Ebola (ZEBOV '76) and Reston Ebola (REBOV '08).

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