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The requirement of leukemia inhibitory factor or epidermal growth factor for pre-implantation embryogenesis

ABSTRACT: In this study, we further verified the role of LIF by looking at gene expressions in morula stage embryos through DNA microarray. Our results showed that LIF knockdown affected 373 gene expressions, and after LIF supplementation we found that the epidermal growth factor (EGF) is most affected by LIF expression. In order to observe the correlation between LIF and EGF, the LIF knockdown embryos were supplemented with various growth factors including LIF, EGF, GM-CSF, TGF, and IGF II. Only LIF and EGF caused the rate of blastocyst development to recover from 52% of control significantly to 83% and 93%, respectively, and all of the parameters, such as the diameter of blastocysts, the numbers of blastomeres, and cells in ICM and TE, to mostly be restored. Moreover, EGF knockdown also impaired blastocyst development which was reversed by LIF or EGF supplementation. These data suggest that the two growth factors EGF and LIF can be compensatory to each other during early embryonic development, and one of them is necessary for sustaining the normal development of preimplantation embryos.

ORGANISM(S): Mus musculus

PROVIDER: GSE71736 | GEO | 2015/09/01

SECONDARY ACCESSION(S): PRJNA291964

REPOSITORIES: GEO

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The requirement of leukemia inhibitory factor or epidermal growth factor for pre-implantation embryogenesis

Project description:In this study, we further verified the role of LIF by looking at gene expressions in morula stage embryos through DNA microarray. Our results showed that LIF knockdown affected 373 gene expressions, and after LIF supplementation we found that the epidermal growth factor (EGF) is most affected by LIF expression. In order to observe the correlation between LIF and EGF, the LIF knockdown embryos were supplemented with various growth factors including LIF, EGF, GM-CSF, TGF, and IGF II. Only LIF and EGF caused the rate of blastocyst development to recover from 52% of control significantly to 83% and 93%, respectively, and all of the parameters, such as the diameter of blastocysts, the numbers of blastomeres, and cells in ICM and TE, to mostly be restored. Moreover, EGF knockdown also impaired blastocyst development which was reversed by LIF or EGF supplementation. These data suggest that the two growth factors EGF and LIF can be compensatory to each other during early embryonic development, and one of them is necessary for sustaining the normal development of preimplantation embryos. Two-condition experiment, mouse embryos vs. LIF-deficiency mouse embryos at morula stage

2015-09-01 | E-GEOD-71736 | biostudies-arrayexpress

Transgenic soybeans expressing human epidermal growth factor

Project description:Each year in the United States, more than 530,000 babies are born prior to full 37 weeks of gestation. One of the major problems associated with prematurity is the development of a condition known as necrotizing enterocolitis (NEC). In 50% of infants with NEC a large amount of damaged and dead intestine must be surgically removed, often resulting in death or lifelong health problems. A growing body of experimental and clinical evidence supports a conclusion that deficient quantities of epidermal growth factor (EGF) leads to the development of NEC. The EGF peptide appears to be fundamental for normal intestinal development and repair. Soy-milk containing engineered EGF may be a suitable therapy if given to premature infants to prevent NEC. Human mature EGF is 6 kDa protein with three intramolecular disulfide bonds. To produce EGF a synthetic codon-optimized gene was transferred to soybean by biolistic transformation. The resulting transgenic lines were regenerated into somatic embryos that were screened by PCR and the production of EGF assayed by ELISA. Crude extracts of somatic embryos producing EGF were assessed for bioactivity by induction of phosphorylation of the EGF receptor in HeLa cells that showed soy-produced EGF exhibited the same activity as authentic EGF. T0 transgenic EGF seeds have showed positive ELISA for EGF demonstrating the feasibility to produce a therapeutic soy-milk to mitigate the development of NEC by premature infants. The next stage of this project will test the efficacy of Soy/EGF in an animal model for short bowel syndrome.

2016-06-22 | PXD003326 | Pride

Actions of FGF2, LIF, and IGF1 on Embryo Survival and Conceptus Elongation following Slow-rate Freezing

Project description:Evaluation of FGF2, LIF, and IGF1 supplementation during embryo culture on conceptus development at day 15.

2022-11-18 | GSE215089 | GEO

embryo_proteome

Project description:We collected over 8000 mouse embryos of each developmental stage (zygote, 2-cell, 4-cell, 8-cell, morula, blastocyst) and performed tandem mass tag (TMT)-based quantitative mass spectrometry and identified nearly 5000 proteins for each stage. In-depth analysis indicates that protein expression profiles of zygote, morula and blastocyst show apparent difference from 2- to 8-cell embryos due to the maternal-totipotent-differentiation transition. We further identified novel factors and proved that they play important roles in determining pre-implantation embryo development

2015-12-10 | MSV000079417 | MassIVE

Systematic in vitro and in vivo characterization of Leukemia-inhibiting factor (LIF)- and Fibroblast growth factor (FGF) -derived porcine induced pluripotent stem cells (piPSC) vs. embryonic stages

Project description:Systematic in vitro and in vivo characterization of Leukemia-inhibiting factor (LIF)- and Fibroblast growth factor (FGF) -derived porcine induced pluripotent stem cells (Cell reprogramming -basic developmental studies in the pig) vs Porcine embryonic stages (Plurisys) Global gene expression analyses and comparisons of LIF piPSC, FGF piPSC, Parental fibroblast line day 7-8 porcine embryo, day 10-11 porcine embryo, day 12-13 porcine embryo. Gene network analyses of piPSC lines, pNF and ICM from day 7-8 porcine embryos.

2017-01-01 | GSE92889 | GEO

The growth factor environment defines distinct pluripotent ground states in novel blastocyst derived stem cells

Project description:Pluripotent stem cell lines can be derived from blastocyst embryos, which yield embryonic stem cell lines (ES cells), as well as the post-implantation epiblast, which gives rise to epiblast stem cell lines (EpiSCs). Remarkably, ES cells and EpiSCs display profound differences in the combination of growth factors that maintain their pluripotent state. Molecular and functional differences between these two stem cell types demonstrate that the tissue of origin and/or the growth factor milieu may be important determinants of the stem cell identity. We explored how developmental stage of the tissue of origin and culture growth factor conditions affect the stem cell pluripotent state. Our findings reveal that novel stem cell lines can be generated from blastocyst embryos with unique functional and molecular properties. We demonstrate that the culture growth factor environment and cell-cell interaction play a critical role in defining several unique and stable stem cell ground states. Keywords: Clonal analysis of FAB-SC gene expression Cell type characterization - 3 separate single cell clones

2010-05-17 | E-GEOD-12519 | biostudies-arrayexpress

miRNA expression profiles from E18th rat embryonic striatal stem cells under conditioned environments

Project description:Background: Striatum is the crucial unit in brain involved in higher-level organizational learning, motor control and cognitive finctions. It also produces dopaminergic neurons, which play an important role in the control of multiple brain functions including voluntary movement and a span of neurobehavioral processes such as, mood, reward, addiction, and stress. Traumatic brain injury (TBI) lead to striatum related persistent cognitive dysfunctions. A number of cell based therapies such as; induced pluripotent stem cells (ips), neural stem cells (NSCs) and mesenchymal stem cells (MSCs), have been investigated so far for their potential application in TBI. But the journey from bench-to-bedside has not yet been covered sucessfully. As their survival and long-term maintenance in vivo has been huddle so far. So to understand the fate of embryonic striatal stem cells (ESSCs) in long-term neurogenesis in vitro, we have studied the neurogenic potential of rat embryonic striatal stem cells from E18th day embryos under conditioned microenvironments. Objectives: To evaluate the neuro-regenerative potential striatum-derived stem cells, we have employed synergestic effects of certain neurotrophins, such as ; EGF, IGF, FGF and LIF as in four combinations (Control/untreated-A, EGF+FGF-B, EGF+FGF+LIF-C, EGF+FGF+IGF-D and EGF+FGF+ IGF +LIF –E) in the microenvironment . To decipher the regulatory miRNAs involved in ESSC-derived neurogenesis, we have performed detailed miRNA profiling for ESSCs at passage 3 for groupD and group E derived ESSCs (based on their performance in vitro). Out data has explored cruicial mIRNAs involved in strital neurogenesis .

2011-08-25 | GSE30276 | GEO

Global gene expression profiles in Oct4-knockdown and Ccna2-knockdown mouse embryos.

Project description:Gene regulation at the maternal-embryonic transition in the pre-implantation mouse embryo is not well understood. We knock down Ccna2 to establish proof-of-concept that antisense morpholino oligonucleotides can be used to target specific genes. We applied this strategy to study Oct4 and discovered that Oct4 is required prior to blastocyst development. Specifically, gene expression is altered as early as the 2-cell stage in Oct4-knockdown embryos. Distinct subsets of genes are differentially expressed between Oct4 and Ccna2-knockdown embryos, and indicated differential functions. Further, a large panel of genes were confirmed to be differentially-expressed in Oct4-knockdown embryos by quantitative, real time RT-PCR. Keywords: gene knockdown

2008-12-31 | GSE13981 | GEO

Human embryo and trophoblast proteome

Project description:Comprehensive quantitative proteomic study of human pre-implantation embryo stages reveal dynamic proteome landscape from M2, 8-cell and blastocyst stage, and during trophoblast stem cell (TS) differentiation. Identified key factors in early human embryos and lineage-specific trophoblast proteome profiles, correlated with transcriptomic analyses. This direct proteomic analysis provides a comprehensive analysis of the dynamic protein expression in human embryos during pre-implantation development and a powerful resource to enable further mechanistic studies on human trophoblast development and function.

2023-07-05 | MSV000092349 | MassIVE

The growth factor environment defines distinct pluripotent ground states in novel blastocyst derived stem cells

2008-10-31 | GSE12519 | GEO

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