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Differential gene expression analysis of iodide treated rat thyroid follicular cell line PCCl3

ABSTRACT: The inhibitory effect of supra physiological iodide concentrations on thyroid hormone synthesis (Wolff-Chaikoff effect) and thyrocyte proliferation is largely know as iodine autoregulation. However, the molecular mechanisms by which iodide excess modulate thyroid functions remains unclear. In this work, we analyzed the rat follicular cell PCCl3 transcriptome profile under untreated and treated conditions with 10-3M sodium iodide (Na/I). Serial Analysis of Gene Expression revealed several transcripts differentially expressed in response to the iodide showing that excess iodide affects almost all aspects of thyroid cell function and differentiation acting on the iodine autoregulatory mechanism through a complex process. Keywords: comparative genomic analysis

ORGANISM(S): Rattus norvegicus

PROVIDER: GSE7174 | GEO | 2007/11/02

SECONDARY ACCESSION(S): PRJNA98247

REPOSITORIES: GEO

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Differential gene expression analysis of iodide treated rat thyroid follicular cell line PCCl3

Project description:The inhibitory effect of supra physiological iodide concentrations on thyroid hormone synthesis (Wolff-Chaikoff effect) and thyrocyte proliferation is largely know as iodine autoregulation. However, the molecular mechanisms by which iodide excess modulate thyroid functions remains unclear. In this work, we analyzed the rat follicular cell PCCl3 transcriptome profile under untreated and treated conditions with 10-3M sodium iodide (Na/I). Serial Analysis of Gene Expression revealed several transcripts differentially expressed in response to the iodide showing that excess iodide affects almost all aspects of thyroid cell function and differentiation acting on the iodine autoregulatory mechanism through a complex process. Keywords: comparative genomic analysis PCCl3 cells were cultured under untreated and treated conditions with 10-3M NaI during 24 hours

2007-11-01 | E-GEOD-7174 | biostudies-arrayexpress

Iodide Inhibits Vascular Endothelial Growth Factor-A Expression in Cultured Human Thyroid Follicles

Project description:A Microarray Search for Effects of Thyrotropin and Iodide on Angiogenesis Factors Objective: Excess iodide has been administered to hyperthyroid patients before thyroid surgery to reduce intraoperative bleeding and oozing. The purpose of this study was to elucidate the mechanism by which iodide reduces blood flow in the hypervascular thyroid gland. Design: Human thyroid follicles were cultured in the presence or absence of thyrotropin (TSH), or in medium containing various concentrations of iodide, and TSH- or iodide-regulated gene expression was analyzed by cDNA microarray. Main outcome: TSH stimulated the expression of thyroglobulin, peroxidase, sodium-iodide symporter, vascular endothelial growth factor (VEGF)-A, VEGF-B, and placental growth factor (PGF) but decreased that of VEGF-C by half. When thyroid follicles were cultured in high-iodide (10-5M) medium, TSH-induced expression of VEGF-A, VEGF-B and PGF was decreased, accompanied by a reduction of VEGF-A release into the medium. Furthermore, expression of putative angiogenesis inhibitors such as urokinase-type plasminogen activator (PLAU) was increased. These findings were confirmed by real-time polymerase chain reaction (PCR) and Northern blot hybridization. Conclusions: We have demonstrated for the first time that iodide at high concentration decreases the expression of the angiogenic factors VEGF-A, VEGF-B, and PGF, accompanied by an increase in the expression of possible anti-angiogenic factors such as PLAU. These proangiogenic and antiangiogenic factors may at least partly account for the iodide-induced decrease in thyroid blood flow.

2008-08-01 | GSE12244 | GEO

Functional inactivation of Thyroid Transcription Factor-1 in PCCl3 thyroid cells

Project description:Thyroid Transcription Factor-1 (TTF-1) is a key regulator of thyroid development and function. In order to identify the genes whose expression depends on TTF-1 transcriptional activity within the thyrocyte we analyzed the consequence of the functional inactivation of this factor in PCCl3 cells. The expression of a fusion protein composed of the DNA binding domain of TTF-1 and of the strong repressive domain of the Engrailed protein (Engr HD) resulted in a dramatic loss of epithelial cell morphology and in proliferation arrest. These changes were reversed when the inhibition of endogenous TTF-1 was relieved. No change was observed when a similar fusion protein containing point mutations abolishing DNA binding activity (Engr HDm) was produced in the cells.

2012-07-01 | GSE35623 | GEO

Glis3 regulates the expression of thyroid hormone synthetic genes in coordination with other thyroid transcription factors

Project description:Comparison of cistromes from PAX8, NKX2.1, and FOXE1 ChIP-Seq analysis using mouse thyroid gland and rat thyrocyte PCCl3 cells revealed that there is a significant overlap between GLIS3 binding regions and those of PAX8, NKX2.1, and FOXE1 in genes associated with thyroid hormone biosynthesis.

2023-04-06 | GSE207777 | GEO

Effect of iodine on early stage thyroid autonomy

Project description:Thyroid autonomy is a frequent cause of thyrotoxicosis in regions with iodine deficiency. Epidemiological data suggest that the prevalence of thyroid autonomy is not only inversely correlated with the ambient iodine supply, but that iodide may also influence the course of pre-existing thyroid autonomy with possibly different effects on thyroid growth and function. Iodine slows TSH effects on thyroid growth stimulation and this effect is more pronounced in thyrocytes with constitutive cAMP activation i.e. in thyroid autonomy. Iodine induced growth alteration in early stage thyroid autonomy is conferred by induction of apoptosis and G2/M arrest. Transcriptome analysis revealed significant modulation of gene networks relevant to cell adhesion, cadherin signalling and ion binding with more pronounced effects in constitutively active FRTL-5 cells compared to normal FRTL-5 cells. The aim was to study iodide-induced changes in global gene expression in an in vitro model of thyroid autonomy. This model makes use of FRTL-5 cells with stable expression of a constitutively activating TSH receptor mutation or wild type TSHR as a control.

2010-12-01 | E-GEOD-22118 | biostudies-arrayexpress

Effect of iodine on early stage thyroid autonomy

2010-12-01 | GSE22118 | GEO

Iodide effect on mRNA expression levels of genes related to immunological function in cultured human thyroid follicles

Project description:We are going to submit a paper entitled “Iodide-induced Chemokines and Genes Related to Immunological Function in Cultured Human Thyroid Follicles” to THYROID

2009-07-14 | GSE16957 | GEO

WDR-23 and SKN-1 Nrf2 coordinate with the BLI-3 dual oxidase in response to iodide-triggered oxidative stress

Project description:Excess iodide could lead to larve arrest to C. elegans, we screened for mutants that can survive in excess iodide and identified gain-of-function mutations in skn-1 and loss-of-function mutations in wdr-23 and bli-3. Genetic study uncover that wdr-23(lf) can interact with bli-3 mutations in a manner different from skn-1(gf). Transcriptome studies suggest that excess iodide causes developmental arrest largely independent of changes in gene expression, and wdr-23(lf) could affect the expression of a subset of genes by a mechanism different from SKN-1 activation.

2018-07-18 | GSE117222 | GEO

RNA-seq of immortalized rat thyrocytes (PCCl3) treated with two thyroid disrupting chemicals (ethylenethiourea and chlorpyrifos) against untreated controls

Project description:The transcriptomic dose-dependent response of rat immortalized thyrocytes to two thyroid disrupting chemicals was investigated by RNA-seq experiment. PCCl3 cells were exposed for one week to different doses of ethylenethiourea (ETU) and chlorpyrifos (CPF) and to their combinations.

2016-12-06 | E-MTAB-5269 | biostudies-arrayexpress

Iodide effect on mRNA expression levels of genes related to immunological function in cultured human thyroid follicles

Project description:We are going to submit a paper entitled âIodide-induced Chemokines and Genes Related to Immunological Function in Cultured Human Thyroid Folliclesâ to THYROID One conditioned experiments, control NaI 10-8M vs. NaI 10-5M , cultured for 3 hours or 48 hours.

2010-08-18 | E-GEOD-16957 | biostudies-arrayexpress

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