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Investigating the Regulation of Hypopharyngeal Gland Activity in Honeybees Under Nutrition and Brood Pheromone Conditions through RNA-seq Transcriptomics

ABSTRACT: The hypopharyngeal gland (HG) is the main site of the synthesis and secretion of royal jelly protein (RJP), and shows high plasticity. To identify differentially expressed genes (DEGs) that affect the development of HG and the synthesis and secretion of RJP, we performed a digital gene expression analysis of 9 d Apis mellifera under different conditions of nutrition and exposure to brood pheromone.Six RNA-seq libraries were generated using RNA extracted from 9 d bee HGs. A total of 2801 DEGs were identified on the basis of at least one pairwise comparison, among which 205, 1617 and 2328 genes were differentially expressed in comparisons between the Pollen group and the Honey group, the Brood group and Pollen group and the Brood group and Honey group respectively. The Brood group exhibited the highest number of DEGs, suggesting that brood pheromone plays a key role in the HG ontogeny. A total of 1991 genes were mapped to 129 canonical signaling pathways found in the Kyoto Encyclopedia of Genes and Genomes (KEGG), and the pathways associated with ribosome function and protein processing were significantly enriched.

ORGANISM(S): Apis mellifera

PROVIDER: GSE71750 | GEO | 2015/08/06

SECONDARY ACCESSION(S): PRJNA292006

REPOSITORIES: GEO

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Investigating the Regulation of Hypopharyngeal Gland Activity in Honeybees Under Nutrition and Brood Pheromone Conditions through RNA-seq Transcriptomics

Project description:The hypopharyngeal gland (HG) is the main site of the synthesis and secretion of royal jelly protein (RJP), and shows high plasticity. To identify differentially expressed genes (DEGs) that affect the development of HG and the synthesis and secretion of RJP, we performed a digital gene expression analysis of 9 d Apis mellifera under different conditions of nutrition and exposure to brood pheromone.Six RNA-seq libraries were generated using RNA extracted from 9 d bee HGs. A total of 2801 DEGs were identified on the basis of at least one pairwise comparison, among which 205, 1617 and 2328 genes were differentially expressed in comparisons between the Pollen group and the Honey group, the Brood group and Pollen group and the Brood group and Honey group respectively. The Brood group exhibited the highest number of DEGs, suggesting that brood pheromone plays a key role in the HG ontogeny. A total of 1991 genes were mapped to 129 canonical signaling pathways found in the Kyoto Encyclopedia of Genes and Genomes (KEGG), and the pathways associated with ribosome function and protein processing were significantly enriched. Hypopharyngeal gland mRNA profiles of 9-day old honeybees under three conditions (one control and two treatments) were generated by deep sequencing, in duplicate, using Illumina Hiseq 2500 platform.

2015-08-06 | E-GEOD-71750 | biostudies-arrayexpress

Investigating the Regulation of Hypopharyngeal Gland Activity in Honeybees Undergoing Harsh Winter Conditions through RNA-seq Transcriptomics Combined with iTRAQ-based Comparative Proteomics

Project description:The honeybee model organism, Apis mellifera carnica, is a hymenopteran with a remarkable ability to withstand cold winters by instinctively entering into hibernation in the hive. Hibernation is characterized by a significant reduction in the metabolic rate, triggered by a drop in the ambient temperature and a shortage of reserves. It provides a token cue that initiates an alternative developmental program that leads to dormancy and switches social behaviors. The age-dependent changes in the generic proteins and substance composition of royal jelly (RJ) have been well documented. However, the molecular mechanism of RJ secretion and the biological consequences of regulation by the hypopharyngeal gland (HG) according to the season remain to be addressed. In this study, large-scale transcriptomic and proteomic approaches were used to detect statistically significant changes in the honeybee proteome and to explore the mechanistic basis for the HG regulation that accompanies hibernation in the winter and arousal in the spring. HG activity was associated with hundreds of gene and protein expression changes in the winter-spring cycle. A systematic study of HG activity and secretion identified 21,441 genes and 2,156 proteins at three time points (i.e., A, winter bees; B, spring bees; and C, retired bees). Of these, 737 genes and 698 proteins were qualified and compared to reveal differential expression patterns, including 197 up-regulated and 291 down-regulated DEGs in group A vs. B, 118 up-regulated and 259 down-regulated DEGs in group A vs. C, and 106 up-regulated and 53 down-regulated DEGs in group B vs. C, respectively. The results were validated by qPCR.Honeybee HG activity was associated with multiple genes regulation.The results present the first global pictures of the regulation of HG activity in winter bee.

2017-12-31 | GSE56910 | GEO

Transcription profiling of brain from honey bees treated with brood pheromone

Project description:Expression profiling of honey bee brains exposed to brood pheromone. Exposure was performed in colonies and young (5 days-old) and old bees (15 days-old) were analyzed .

2008-12-31 | E-TABM-512 | biostudies-arrayexpress

cGMP modulates responses to queen mandibular pheromone in worker honey bees

Project description:Responses to social cues, such as pheromones, can be modified by genotype, physiology, or environmental context. Honey bee queens produce a pheromone (queen mandibular pheromone; QMP) which regulates many aspects of worker bee behavior and physiology. Forager honey bees are less responsive to QMP than young nurse bees engaged in brood care, suggesting that physiological changes associated with behavioral maturation may modulate response to this pheromone. Since cGMP is a major regulator of behavioral maturation in honey bee workers, we examined its role in modulating worker responses to QMP. Treatment with a cGMP analog, 8-Br-cGMP, resulted in significant reductions in both behavioral and physiological responses to QMP in young caged workers. Treatment significantly reduced attraction to QMP (the retinue response) and inhibited the QMP-mediated increase in vitellogenin levels in the fat bodies of worker bees. Genome-wide analysis of brain gene expression patterns demonstrated that cGMP has a larger effect on expression levels than QMP, and that QMP has specific effects in the presence of cGMP, suggesting that some responses to QMP may be dependent on an individual beesM-^R physiological state. Several functional gene categories were significantly differentially expressed, including genes involved in regulating GTPase activity, phototransduction, immunity, and carboxylic acid transmembrane transporter activity. Overall, our data suggest that cGMP-mediated processes play a large role in modulating responses to queen pheromone in honey bees, at the behavioral, physiological and molecular levels.

2011-06-15 | E-MEXP-3135 | biostudies-arrayexpress

Transcriptome of Varroa destructor life cycle

Project description:To study the underlying molecular mechanisms during the Varroa destructor life cycle, we carried out transcriptomic profiling of seven stages: young mites (collected from P8 to P9 brood cells), phoretic mites (collected on adult bees), arresting mites (collected in unsealed L5 brood cells), pre-laying mites (collected from sealed brood cells containing moving larva), laying mites (collected from sealed brood cells containing pre-pupae), post-laying mites (collected from capped brood cells containing purple-eye and white-body pupae P5), emerging mites (collected from P8 to P9 brood cells). In addition, we sampled non-reproducing mites (collected from P5 brood cells, but without offspring), males (collected from P8 to P9 brood cells), and phoretic mites artificially reared in cages with adult bees. This study was performed using Apis mellifera L. honey bee colonies naturally infested by Varroa destructor mites. Adult mites were collected from 4 unrelated colonies.

2018-04-11 | GSE100554 | GEO

GNPS - <Chemical diversity in a stingless bee-plants symbiosis>

Project description:LC-ESI-MS/MS data files (positive and negative ion monitoring modes) of samples collected from three colonies of the stingless bee Scaptotrigona depilis: honey, fermented pollen, nurse bees, pupae, larvae, larval food from brood cells with eggs, larval food from brood cells with larvae, brood fungus, cerumen, propolis, colony entrance and the controls.

2019-08-14 | MSV000084208 | GNPS

GNPS - Fipronil, fipronil desulfinyl and fipronil sulfone analyses of NZ bees and bee products

Project description:A QTOF-based project on detecting possible fipronil, fipronil desulfinyl and fipronil sulfone contamination of bees and bee products (honey, brood, collected pollen etc) from wasp treatment areas that had been exposed to Vespex(R) toxic wasp bait.

2018-04-08 | MSV000082256 | GNPS

Antennae transcriptomics of Varroa-hygienic and Nurse/Forager bees

Project description:The Varroa mite represents the main threat of honey bees (Apis mellifera). Bees from some colonies can limit the proliferation of this parasite by detecting and removing parasitized brood, such behavior is defined as Varroa sensitive Hygiene (VSH). This is an important issue for selecting colonies that can survive Varroa outbreaks. We therefore study the molecular meachnisms underlying this behavior by comparing the antennae transcriptomic profile of VSH and non-VSH bees. Those profiles were further compared to to the profiles of nurses and forager profiles involved in brood care and food collection, respectively.

2017-02-09 | GSE63613 | GEO

Proteomics of hygienic honey bee (A. mellifera) antenna lateralization

Project description:In other experiments, we found that hygienic honey bees show lateralized responses to odors associated with brood disease. Bees are also known to have more olfactory sensilla on their right antenna compared to their left. Following this, we performed proteomics on right and left antennae of hygienic honey bees in order to identify a molecular basis for lateralization (N = 5); however, we did not identify any significantly different proteins.

2017-12-07 | PXD005242 | Pride

Transcription profiling of young bees raised in colonies with a queen, with queen mandibular pheromone (QMP), or without a queen.

Project description:This experiment was designed to identify gene expression differences in young bees raised in colonies with a queen, with queen mandibular pheromone (QMP), or without a queen. The source colony was split into three colonies, allocating roughly equal quantities of adult bees, brood, and pollen and nectar stores. One colony retained the original queen (QR), one was left queenless (QL), and the third was given a strip that contained 10 Qeq (QMP+), a dose shown to mimic a live queen (20). The three colonies were transferred to a different apiary > 2 mi away so they would not return to the site of their natal hive. Prior to the colony split, bees (N ~1500) were collected 0-36 h after eclosion, marked on the dorsal thorax with a paint dot (Testors Paint), and ~500 were placed in each of the three colonies. Two days later, the marked bees were collected (N = 100). Bees were collected into liquid nitrogen, and heads were stored as above.

2004-10-15 | E-MEXP-80 | biostudies-arrayexpress

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