Genomics

Dataset Information

154

Spo11-oligo mapping in Saccharomyces species (S. paradoxus, S. mikatae, S. kudriavzevii) and wild-derived S. cerevisiae strains (YPS128, UWOPS03-461.4)


ABSTRACT: The nonrandom distribution of meiotic recombination shapes heredity and genetic diversification. A widely held view is that individual hotspots - favored sites of recombination initiation - are always ephemeral because they evolve rapidly toward extinction. An alternative view, often ignored or dismissed as implausible, predicts conservation of the positions of hotspots if they are chromosomal features under selective constraint, such as gene promoters. Here we empirically test opposite predictions of these theories by comparing genome-wide maps of meiotic recombination initiation from widely divergent species in the Saccharomyces clade. We find that the frequent overlap of hotspots with promoters is true of the species tested and, consequently, hotspot positions are well conserved. Remarkably, however, the relative strength of individual hotspots is also highly conserved, as are larger-scale features of the distribution of recombination initiation. This stability, not predicted by prior models, suggests that the particular shape of the yeast recombination landscape is adaptive, and helps in understanding evolutionary dynamics of recombination in other species. Overall design: Ten samples total: two biological replicate Spo11-oligo maps of each of the following: wild-derived S. cerevisiae strain YPS128, wild-derived S. cerevisiae UWOPS03-461.4, S. paradoxus YPS138, S. mikatae IFO1815, S. kudriavzevii ZP591

INSTRUMENT(S): Illumina HiSeq 2500 (Saccharomyces cerevisiae)

SUBMITTER: Isabel Lam 

PROVIDER: GSE71887 | GEO | 2015-08-17

SECONDARY ACCESSION(S): PRJNA292494

REPOSITORIES: GEO

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Publications

Nonparadoxical evolutionary stability of the recombination initiation landscape in yeast.

Lam Isabel I   Keeney Scott S  

Science (New York, N.Y.) 20151101 6263


The nonrandom distribution of meiotic recombination shapes heredity and genetic diversification. Theoretically, hotspots--favored sites of recombination initiation--either evolve rapidly toward extinction or are conserved, especially if they are chromosomal features under selective constraint, such as promoters. We tested these theories by comparing genome-wide recombination initiation maps from widely divergent Saccharomyces species. We find that hotspots frequently overlap with promoters in th  ...[more]

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