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Identification of downstream genes regulated by OsMADS2

ABSTRACT: To gain mechanistic insights on OsMADS2 functions in rice spikelet development, we have employed global expression profilling to identify the genes regulated by OsMADS2. Transgenic rice panicle RNAs were compared with the wild type panicle RNA of similar developmental stage. Genes downregulated in transgenic lines will be those which expression requires functional OsMADS2 whereas upregulated genes will need OsMADS2 for their repression. Keywords: Comparative expression profile analysis

ORGANISM(S): Oryza sativa Oryza sativa Japonica Group

PROVIDER: GSE7192 | GEO | 2007/07/31

SECONDARY ACCESSION(S): PRJNA98233

REPOSITORIES: GEO

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Transcription profiling of rice panicle RNAs were compared with the wild type panicle RNA of similar developmental stage to identify of downstream genes regulated by OsMADS2

Project description:To gain mechanistic insights on OsMADS2 functions in rice spikelet development, we have employed global expression profilling to identify the genes regulated by OsMADS2. Transgenic rice panicle RNAs were compared with the wild type panicle RNA of similar developmental stage. Genes downregulated in transgenic lines will be those which expression requires functional OsMADS2 whereas upregulated genes will need OsMADS2 for their repression. Experiment Overall Design: Two independent biological RNA pools of dsRNAiOsMADS2 transgenic panicles were compared with a single wild type RNA pool in two different hybridizations. A third hybridization was performed with reciprocally dye-lebeled wild type RNA and one of the mutant RNA.

2008-06-15 | E-GEOD-7192 | biostudies-arrayexpress

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Project description:In rice (Oryza sativa L.), the number of panicles, spikelets per panicle and grain weight are important components of grain yield. These characteristics are controlled by quantitative trait loci (QTLs) and are derived from variation inherent in crops.The identification of different yield related QTLs facilitates an understanding of the mechanisms involved in cereal crop yield, and may have utility in improving grain yield in cereal crops. an understanding of the mechanisms involved in cereal crop yield, and may have utility in improving grain yield in cereal crops. In the present study, We cloned and characterized a large-panicle QTL, and confirmed that the newly identified gene OsEBS (enhancing biomass and spikelet number) increased plant height, leaf size and spikelet number per panicle, leading to an average of 37.62% increase in total grain yield per plant. trait loci (QTLs) and are derived from variation inherent in crops. OsEBS-transgenic rice B10201 and B10301 and control Guichao2

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ABERRANT PANICLE ORGANIZATION2 controls multiple steps in panicle formation through common direct-target genes

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Expression data from OsEBS-transgenic rice B10201 and B10301 and control Guichao2

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Down-regulated expression of OsDCL3b affects small RNA biogenesis and panicle development in rice

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