Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

The Host Response to a Clinical MDR Mycobacterial Strain Cultured in a Detergent-Free Environment: A Global Transcriptomics Approach

ABSTRACT: We infect bone marrow derived macrophages (BMDMs) with Mycobacterium cultured with and without Tween 80 and asses the host response to these bacteria through RNAseq

ORGANISM(S): Mus musculus

PROVIDER: GSE72003 | GEO | 2015/08/13

SECONDARY ACCESSION(S): PRJNA292705

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

The Host Response to a Clinical MDR Mycobacterial Strain Cultured in a Detergent-Free Environment: A Global Transcriptomics Approach

Project description:We infect bone marrow derived macrophages (BMDMs) with Mycobacterium cultured with and without Tween 80 and asses the host response to these bacteria through RNAseq Uninfected BMDMs served as controls, BMDMs infected with detergent-free M.tb (designated RNT), BMDMs infected with Tween cultured m.tb (designated RT). 3 groups with 3 biological replicates, each analysed in triplicate.

2015-08-13 | E-GEOD-72003 | biostudies-arrayexpress

The Host Response to a Clinical MDR Mycobacterial Strain Cultured in a Detergent-Free Environment: A Global Transcriptomics Approach.

Project description: Not available

| S-EPMC4824497 | biostudies-literature

Mtb Rv2869c mutant/WT +/- detergent

Project description:The purpose of this experiment is to examine gene expression controlled by Rv2869c in the presence or absence of detergent (Tween 80) in the culture medium. Keywords: parallel sample

2005-07-22 | GSE2561 | GEO

RNAseq Reveals Virulence-Specific Host Responses to M. tuberculosis infection

Project description:We infect bone marrow derived macrophages (BMDMs) with either a Hyper or Hypovirulent Mycobacterium tuberculolsis strain and assess the host response to these bacteria through RNAseq Uninfected BMDMs served as controls, BMDMs infected with Hypervirulent M.tb (designated R55), BMDMs infected with Hypovirulent m.tb (designated R50). 3 groups with 3 biological replicates, each analysed in triplicate.

2016-03-01 | E-GEOD-78706 | biostudies-arrayexpress

RNAseq Reveals Virulence-Specific Host Responses to M. tuberculosis infection

2016-03-01 | GSE78706 | GEO

Effect of small molecule inhibitors on transcriptome of B6.Sst1S bone marrow derived macrophages stimulated with TNF and infected with virulent Mycobacterium tuberculosis

Project description:We investigated candidate host-directed therapies for tuberculosis in vitro. BMDMs from tuberculosis susceptible mice B6.Sst1S were stimulated with TNF and infected with Mtb. The infected macrophage were treated with Rocaglamide A, ISRIB and JNK inhibitor SP600125 alone or in combinations to asses the inhibitors' effectas on macrophage transcriptome.

2023-10-04 | GSE237582 | GEO

Effect of hypoxia on Mycobacterium tuberculosis-host phagocyte interactions

Project description:Mtb appears to have developed specialized biomolecular infrastructure to survive and persist within granulomas, where it is subjected to a diverse set of stress conditions. One of these stress conditions is hypoxia. We hypothesized that host cell response is radically altered with hypoxia stressed Mtb and designed in-vitro experiments to study this phenomenon. Hypoxia-stressed as well as aerobically grown Mtb were used to infect rhesus macaque bone marrow derived macrophages (Rh-BMDMs) and the host global transcriptional response compared. Using 4 x44 k Agilent arrays specific for rhesus macaque genome, we tested in biological duplicate the effect of aerogically grown Mtb on rhesus macaque BMDMs and compared this to the corresponding effect of the hypoxia-conditioned Mtb on rhesus macaque BMDMs

2013-05-22 | E-GEOD-47163 | biostudies-arrayexpress

Ligand-free SpSLC9C1 in detergent

Project description: Not available

| EMPIAR-11629 | biostudies-other

Detergent-free mass spectrometry of membrane protein complexes.

Project description: Not available

| S-EPMC3868940 | biostudies-literature

Detergent-free systems for structural studies of membrane proteins.

Project description: Not available

| S-EPMC8276625 | biostudies-literature