Genomics

Dataset Information

0

The DNA-binding inhibitor Id3 regulates IL-9 production in CD4+ T cells


ABSTRACT: The molecular mechanisms by which signaling via transforming growth factor-β (TGF-β) and interleukin 4 (IL-4) control the differentiation of IL-9-producing CD4+ helper T cells (TH9 cells) remain incompletely understood. We found here that the DNA-binding inhibitor Id3 regulated TH9 cell differentiation, as deletion of Id3 increased IL-9 production from CD4+ T cells. Mechanistically, TGF-β1 and IL-4 downregulated Id3 expression, and this process required the kinase TAK1. A reduction in Id3 expression enhanced binding of the transcription factors E2A and GATA-3 to the Il9 promoter region, which promoted Il9 transcription. Notably, Id3’s control of TH9 differentiation regulated anti-tumor immunity in an experimental melanoma-bearing model in vivo and also in human CD4+ T cells in vitro. Thus, our study reveals a previously unrecognized TAK1–Id3–E2A–GATA-3 pathway that regulates TH9 differentiation.

ORGANISM(S): Mus musculus

PROVIDER: GSE72051 | GEO | 2015/08/14

SECONDARY ACCESSION(S): PRJNA292820

REPOSITORIES: GEO

Similar Datasets

2017-02-06 | GSE86542 | GEO
2022-08-09 | GSE182472 | GEO
| E-GEOD-41317 | biostudies-arrayexpress
2014-02-20 | GSE41317 | GEO
2019-02-05 | GSE123501 | GEO
2019-01-08 | GSE106926 | GEO
| E-GEOD-30518 | biostudies-arrayexpress
2011-07-31 | GSE30518 | GEO
| E-GEOD-79607 | biostudies-arrayexpress
| E-GEOD-54697 | biostudies-arrayexpress