Dataset Information


Genome-wide map of H3K4me3 before and after methionine restriction in HCT116 cells.

ABSTRACT: The goals of this study were to compare the distribution of H3K4me3 before and after methionine restriction to understand how nutrient availability and alterations in methionine metabolism affect the epigenetic state and regulate gene transcription. To do this we performed ChIP-seq with an H3K4me3 antibody and RNA-seq using PolyA+ mRNA. Overall design: We compared genome-wide H3K4me3 and mRNA profiles generated by Next Generation Sequencing (NGS) between +Methionine and -Methionine conditions after 24 hours in HCT116

INSTRUMENT(S): Illumina HiSeq 2500 (Homo sapiens)

SUBMITTER: Jason W Locasale  

PROVIDER: GSE72131 | GEO | 2015-08-17



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S-adenosylmethionine (SAM) and S-adenosylhomocysteine (SAH) link one-carbon metabolism to methylation status. However, it is unknown whether regulation of SAM and SAH by nutrient availability can be directly sensed to alter the kinetics of key histone methylation marks. We provide evidence that the status of methionine metabolism is sufficient to determine levels of histone methylation by modulating SAM and SAH. This dynamic interaction led to rapid changes in H3K4me3, altered gene transcription  ...[more]

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