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Expression data from pooled biological replicates (n=6) from MEF2D knockdown in neonatal rat ventricular myocytes (NRVMs)

ABSTRACT: Differentiation and maintenance of cardiac muscle is a complex biological process. MEF2D appears to play an important role in the regulation of cardiomyocyte homeostasis. We knocked down expression of MEF2D in NRVMs, and assessed global expression pattern changes in MEF2D knockdown against a negative control.

ORGANISM(S): Rattus norvegicus

PROVIDER: GSE72157 | GEO | 2015/08/31

SECONDARY ACCESSION(S): PRJNA293215

REPOSITORIES: GEO

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Expression data from pooled biological replicates (n=6) from MEF2D knockdown in neonatal rat ventricular myocytes (NRVMs)

Project description:Differentiation and maintenance of cardiac muscle is a complex biological process. MEF2D appears to play an important role in the regulation of cardiomyocyte homeostasis. We knocked down expression of MEF2D in NRVMs, and assessed global expression pattern changes in MEF2D knockdown against a negative control. NRVMs were extracted from 1 to 2 day old rat pups and were allowed to recover for 24 hours before being transduced with shRNA viral vectors to knockdown expression of MEF2D or LacZ (negative control). After 3 days, total RNA was harvested for Affymetrix global gene expression microarray analysis. Each array was pooled RNA from six biological replicates.

2015-08-31 | E-GEOD-72157 | biostudies-arrayexpress

RNA-seq transcriptonal profiling in neonatal rat ventricular myocytes under hypertrophic growth

Project description:Heart failure is a leading cause of death in US. Hypertension is one of the most important risk factor of heart failure. In the presence of high blood pressure, the heart manifests hypertrophic growth to ameliorate ventricular wall stress. This once adaptive response may progress into decompensation and heart failure. The precise mechanisms governing this transition remain elusive. Here, we aimed to identify novel signaling pathways in cardiac hypertrophic growth. Primary neonatal rat ventricular myocytes (NRVMs) were isolated from 1-2 days old rats and treated with phenylephrine or IGF-1. Total RNA was isolated for RNA-seq analysis.

2020-11-16 | GSE153763 | GEO

Expression data from individual MEF2A isoform knockdown in neonatal rat ventricular myocytes (NRVMs)

Project description:Regulation of homeostasis and development of cardiac muscle tissues is controlled by a core set of transcription factors. The MEF2 family plays a critical role in these processes. We knocked down expression of MEF2 isoforms in a primary cell culture model of cardiomyocytes and assessed global expression pattern changes between MEF2 knockdowns, and with a negative control.

2017-09-08 | GSE92861 | GEO

Conversion of neonatal myocytes to pacemakers by viral transduction with Tbx18

Project description:In this study, we performed two-dimensional liquid chromatography coupled to tandem mass spectrometry (2D-LC-MS/MS) to characterize the global transcriptome and proteome changes in Tbx18-iPMs compared to control, GFP-overexpressing neonatal rat ventricular myocytes (NRVMs). 67% of protein abundances changed significantly (4,475 of 6,661 proteins; p<0.05) at a false discovery rate of 1.6%. Downregulation was broad among metabolic pathways, such as TCA cycle, oxidative phosphorylation, glycolysis and fatty acid oxidation. Ion channels associated with ventricular excitation-contraction coupling (sodium, calcium, and potassium channels, Connexin 43, among others) were likewise downregulated. Pacemaker phenotype was characterized by upregulation of the pacemaker current channel (HCN4) but also mechnosensitive Piezo1, Trp channels Trpp2 (PKD2) and TrpM7, and Connexin45. Consistent with emergent pacemaker morphology, there was broad upregulation of cytoskeletal proteins and their small GTPase regulators. Extracellular matrix and growth factor/cytokine/interferon genes were also dynamically upregulated, indicating that Tbx18-iPMs are primed for sinoatrial node integration.

2022-08-26 | PXD013351 | Pride

Gene expression signatures of MEF2D-silencing hepatocellular carcinoma cells

Project description:Global expression profiles in Huh7 after infection with Lv-shMEF2D and Lv-scrambled, as well as transfection with siRNA-MEF2D and siRNA-control, were compared to investigate the molecular mechanisms involved in MEF2D-mediated regulation of cell cycle. In order to investigate the molecular mechanisms involved in MEF2D-mediated regulation of cell cycle, we assessed gene expression profiles in Huh7 cells after infection with Lv-shMEF2D and Lv-scrambled, as well as transfection with siRNA-MEF2D and siRNA-control, by cDNA microarray. Analysis of global mRNA expression profile indicated a shift toward G2/M arrest in the cells after downregulating MEF2D expression. The genes that inhibit G2/M transition were found to be expressed in high level in MEF2D-downregulated group, as compared with control group. Meanwhile, mRNA abundance of G2/M transition-promoting genes, except CDC2 and CDC25C, was reduced when MEF2D expression was depressed in Huh7 cells

2015-04-07 | E-GEOD-37123 | biostudies-arrayexpress

Gene expression profile of Mef2d KO retina at P14

Project description:To analyze the expression profile in the Mef2d KO retina, we performed a microarray analysis using wild-type and Mef2d KO retina at P14. We performed a microarray analysis using wild-type and Mef2d KO retina at P14, and compared the expression profiles.

2015-07-01 | E-GEOD-64861 | biostudies-arrayexpress

Transcription profiling of rat neonatal ventricular myocytes

Project description:Title: Regulation of gene expression through mitogen-activated protein kinase cascades in cardiac myocytes. Description: The aim of this study is to identify the changes in gene expression induced in rat neonatal ventricular myocytes, a well-established cell culture model, by endothelin-1, a known hypertrophic agonist, and to determine which of the changes are mediated through the ERK cascade. This continues TKAC^Ys previous study of the effects of oxidative stress, which induces cardiac myocyte apoptosis.

2006-10-01 | E-MIMR-3 | biostudies-arrayexpress

Selective Class IIa HDAC inhibitor alters gene expression in NRVMs

Project description:Expression profiling of neonatl rat ventricular cardiomyocytes (NRVMs) upon the treatement of Class IIa HDAC inhibitor

2019-04-23 | GSE130150 | GEO

RNA-seq for gene expression changes upon silencing MEF2D-fusion in a MEF2D-HNRNPUL1 fusion-expressing Kasumi-7 cell line

Project description:To explore the mechanisms underlying the maintenance of acute lymphoblastic leukemia harboring fusion genes involving MEF2D transcription factor, the MEF2D-fusion was silenced by shRNA and the resulting gene expression changes were analyzed by RNA-seq.

2020-06-30 | E-MTAB-8480 | biostudies-arrayexpress

RNAseq of NRVMs infected with AdCryABR120G by the manipulation of Ube2v1 expression

Project description:Neonatal rat ventricle myocytes (NRVMs) were infected with CryABR120G adenovirus. Knockdown or overexpression of Ube2v1 was performed by siUbe2v1 transfection or AdUbe2v1 infection in CryABR120G-expressing cells. Total RNA was extracted from NRVMs of four groups: CTR (untreated control), R120G (AdCryABR120G infection), RSIV1 (AdCryABR120G infection with siUbe2v1 transfection), and RADV1 (coinfection with AdCryABR120G and AdUbe2v1), in triplicate, for gene expression profiling.

2020-08-10 | GSE144974 | GEO

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