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The immunomodulatory effects of soluble receptor of activated nuclear factor kappa ligand (sRANKL) on monocyte-derived macrophages

ABSTRACT: To explore the effects of sRANKL on tumor-associated macrophages, we first sorted CD14+ monocytes from peripheral blood mononuclear cells of healthy donors using magnetic-activated cell sorting and then induced their differentiation into macrophages by culture with M-CSF for 5 days. We then stimulated these macrophages with IL-4 (20 ng/ml) together with or without 100 ng/ml of sRANKL for 6 hours and examined gene expression using a cDNA microarray.

ORGANISM(S): Homo sapiens

PROVIDER: GSE72292 | GEO | 2015/08/24

SECONDARY ACCESSION(S): PRJNA293673

REPOSITORIES: GEO

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The immunomodulatory effects of soluble receptor of activated nuclear factor kappa ligand (sRANKL) on monocyte-derived macrophages

Project description:To explore the effects of sRANKL on tumor-associated macrophages, we first sorted CD14+ monocytes from peripheral blood mononuclear cells of healthy donors using magnetic-activated cell sorting and then induced their differentiation into macrophages by culture with M-CSF for 5 days. We then stimulated these macrophages with IL-4 (20 ng/ml) together with or without 100 ng/ml of sRANKL for 6 hours and examined gene expression using a cDNA microarray. sRANKL stimulated gene expression on monocyte-derived macrophages was measured at 6 hours after exposure to doses of 100 ng/ml sRANKL.

2015-08-24 | E-GEOD-72292 | biostudies-arrayexpress

The immunomodulatory effects of IL-4 on monocyte-derived macrophages

Project description:To explore the effects of IL-4 on tumor-associated macrophagess, we first sorted CD14+ monocytes from peripheral blood mononuclear cells of healthy donors using magnetic-activated cell sorting and then induced their differentiation into macrophages by culture with M-CSF for 5 days. We then stimulated these macrophages with or without IL-4 (20 ng/ml) for 6 hours and examined gene expression using a cDNA microarray. IL-4 stimulated gene expression on monocyte-derived macrophages was measured at 6 hours after exposure to doses of 20 ng/ml IL-4.

2015-12-10 | E-GEOD-75829 | biostudies-arrayexpress

The immunomodulatory effects of IL-4 on monocyte-derived macrophages

2015-12-10 | GSE75829 | GEO

The immunomodulatory effects of periostin on monocyte-derived macrophages

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2015-07-14 | E-GEOD-70821 | biostudies-arrayexpress

The immunomodulatory effects of periostin on monocyte-derived macrophages

2015-07-14 | GSE70821 | GEO

Transcriptional effects of activation stimuli on M-CSF-derived macrophages

Project description:Analysis of the effect of various activation stimuli on the transcriptional signature of human M-CSF-dependent monocyte-derived macrophages. Methods: Human Peripheral Blood Mononuclear Cells (PBMC) were isolated from buffy coats from donors over a Lymphoprep gradient according to standard procedures. Monocytes were purified from PBMC by magnetic cell sorting using anti-CD14 microbeads (>95% CD14+ cells). Monocytes from three independent donors (0.5 x 106 cells/ml, >95% CD14+ cells) were cultured in RPMI 1640 supplemented with 10% fetal bovine serum (FBS) at 37°C in a humidified atmosphere with 5% CO2 and 21% O2 for 7 days in the presence of 10 ng/ml M-CSF, with cytokine addition every two days. After 7 days, macrophages were either untreated (CNT) or treated with 10 ng/ml E. coli 055:B5 lipopolysaccharide (LPS), 100 ng/ml of the TLR7 agonist CL264 (CL), BSA or 200 mM palmitate (PAL) for 0.5h, 2h, 4h or 12h.

2020-12-09 | GSE156921 | GEO

Transcriptional effects of MAF- and MAFB-siRNAs on M-CSF-derived macrophages

Project description:Analysis of the role of transcriptions factors MAF and MAFB on the phenotypic profles of human M-CSF-derived macrophages. Methods: Human Peripheral Blood Mononuclear Cells (PBMC) were isolated from buffy coats from donors over a Lymphoprep gradient according to standard procedures. Monocytes were purified from PBMC by magnetic cell sorting using anti-CD14 microbeads (>95% CD14+ cells). Monocytes (0.5 x 106 cells/ml, >95% CD14+ cells) were cultured in RPMI 1640 supplemented with 10% fetal bovine serum (FBS) for 7 days in the presence of 10 ng/ml M-CSF to generate M-CSF-polarized macrophages (M-MØ). Macrophages were differentiated from peripheral blood monocytes from 3 healthy donors with M-CSF (M-MØ) to generate anti-inflammatory M-MØ. Macrophages were transfected with either Control siRNA or MAFB-specific siRNA or MAF-specific siRNA for 24h and global gene expression was analysed by RNA-Seq.

2020-12-09 | GSE155719 | GEO

Transcriptome of human primary macrophages from healthy controls after long-term myelin phagocytosis

Project description:CD14+ monocytes were isolated from the blood of healthy human donors by Ficoll density-gradient centrifugation, magnetic cell sorting and LS column systems. CD14+ monocytes were in vitro differentiated to macrophages by culturing the cells for 7 days in RPMI medium containing 10% FCS, 1% penicillin/streptomycin, 1% glutamine, 1% Fungizone and supplemented with 50 ng/ml human macrophage-colony stimulating factor (M-CSF) in the presence or absence of myelin isolated from brain tissue of 6-8 week-old wild-type C57BL6/J mice.

2023-11-22 | GSE245015 | GEO

Transcriptional effects of IL-10 neutralization on LPS-activated M-CSF-derived macrophages

Project description:Analysis of the role of IL-10 on the transcriptional signature of LPS-activated human M-CSF-dependent monocyte-derived macrophages. Methods: Human Peripheral Blood Mononuclear Cells (PBMC) were isolated from buffy coats from donors over a Lymphoprep gradient according to standard procedures. Monocytes were purified from PBMC by magnetic cell sorting using anti-CD14 microbeads (>95% CD14+ cells). Monocytes from three independent donors (0.5 x 106 cells/ml, >95% CD14+ cells) were cultured in RPMI 1640 supplemented with 10% fetal bovine serum (FBS) at 37°C in a humidified atmosphere with 5% CO2 and 21% O2 for 7 days in the presence of 1000 U/ml M-CSF, with cytokine addition every two days. Then, monocyte-derived macrophages were treated with an anti-IL-10 blocking antibody (anti-IL10) or an isotype-matched antibody (IgG2b) at 2.5 micrograms/ml for 1 hour, and exposed to LPS (10 ng/ml). After 4 hours of LPS treatment, cells were lysed and RNA isolated for transcriptional analysis.

2022-06-08 | GSE181250 | GEO

Transcriptional effects of siRNA-mediated STAT3 knockdown on LPS-activated M-CSF-derived macrophages

Project description:Analysis of the role of STAT3 on the transcriptional signature of LPS-activated human M-CSF-dependentmonocyte-derived macrophages. Methods: Human Peripheral Blood Mononuclear Cells (PBMC) were isolated from buffy coats from donors over a Lymphoprep gradient according to standard procedures.monocytes were purified from PBMC by magnetic cell sorting using anti-CD14 microbeads (>95% CD14+ cells).monocytes from three independent donors (0.5 x 106 cells/ml, >95% CD14+ cells) were cultured in RPMI 1640 supplemented with 10% fetal bovine serum (FBS) at 37°C in a humidified atmosphere with 5% CO2 and 21% O2 for 5 days in the presence of 1000 U/ml M-CSF, with cytokine addition every two days. Then,monocyte-derived macrophages were transfected with STAT3-specific siRNA or control siRNA, kept in culture for 48 hours, and then stimulated (or not) with LPS (10 ng/ml). After 4 hours, cells were lysed and RNA isolated for transcriptional analysis.

2022-06-08 | GSE180897 | GEO

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