Transcriptomics,Genomics

Dataset Information

159

Global microRNA expression profiling of mouse livers following ischemia-reperfusion injury at different stages. [mRNA]


ABSTRACT: Hepatic ischemia reperfusion injury is a dynamic process consisting of two stages: ischemia and reperfusion, and triggers a cascade of physiological and biochemical events. Given the important role of microRNAs in regulating gene expression, we analyzed gene expression changes in mouse livers at sham control, ischemia stage, and reperfusion stage. We generated global expression profiles of microRNA and mRNA genes in mouse livers subjected to ischemia reperfusion injury at the three stages, respectively. Comparison analysis showed that reperfusion injury had a distinct expression profile whereas the ischemia sample and the sham control were clustered together. Consistently, there are 69 differentially expressed microRNAs between the reperfusion sample and the sham control whereas 28 differentially expressed microRNAs between the ischemia sample and the sham control. We further identified two modes of microRNA expression changes in ischemia reperfusion injury. Functional analysis of both the differentially expressed microRNAs in the two modes and their target mRNAs revealed that ischemia injury impaired mitochondria function, nutrient consumption, and metabolism process. In contrast, reperfusion injury led to severe tissue inflammation that is predominantly an innate-immune response in the ischemia reperfusion process. Our staged analysis of gene expression profiles provides new insights into regulatory mechanisms of microRNAs in mouse hepatic ischemia reperfusion injury. Overall design: Liver mRNA profiles of male mouse liver under Sham, warm ischemia (WI) and Ischemia-reperfusion (IR) conditions were generated by deep-sequencing using Solexa/Illumina platform.

INSTRUMENT(S): Illumina HiSeq 2000 (Mus musculus)

SUBMITTER: Weisheng Zheng  

PROVIDER: GSE72312 | GEO | 2015-10-01

SECONDARY ACCESSION(S): PRJNA293736

REPOSITORIES: GEO

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Publications

Global MicroRNA Expression Profiling of Mouse Livers following Ischemia-Reperfusion Injury at Different Stages.

Zheng Weisheng W   Men Hewei H   Li Jing J   Xing Yu Y   Wu Bin B   Wang Zhenglu Z   Li Junjie J   Teng Dahong D   Shi Yuan Y   Li Jiang J   Jiang Pu P   Cai Jinzhen J  

PloS one 20160209 2


Hepatic ischemia-reperfusion injury is a dynamic process consisting of two stages: ischemia and reperfusion, and triggers a cascade of physiological and biochemical events. Given the important role of microRNAs in regulating gene expression, we analyzed gene expression changes in mouse livers at sham control, ischemia stage, and reperfusion stage. We generated global expression profiles of microRNA and mRNA genes in mouse livers subjected to ischemia-reperfusion injury at the three stages, respe  ...[more]

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