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FACS-isolated Lin-CD24-CD49f-Sca-1+stromal cells from adult mouse proximal and distal prostatic ducts

ABSTRACT: We have noticed that the proliferative potential of epithelial cells in the mouse proximal prostatic ducts is less than those at the distal prostatic ducts. We were curious whether the tissue microenvironment in the two anatomic regions play a role in the distinct epithelial proliferative indices. So we isolated respective stromal cells in the two regions and performed an RNA-seq analysis.

ORGANISM(S): Mus musculus

PROVIDER: GSE72318 | GEO | 2019/06/21

REPOSITORIES: GEO

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Gene expression profiling of prostate basal cells at proximal and distal ducts

Project description:We have noticed that the proliferative potential of epithelial cells in the mouse proximal prostatic ducts is less than those at the distal prostatic ducts. To determine whether specific signaling is differentially activated in distal and proximal prostate basal cells, we isolated respective basal cells in the two regions and performed an RNA-seq analysis.

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Gene expression profile of zebrafish renal collecting ducts

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2012-08-24 | E-GEOD-32363 | biostudies-arrayexpress

Ablating Lgr5-expressing prostatic stromal cells activates the ERK-mediated mechanosensory signaling and disrupts prostate tissue homeostasis

Project description:The heterogeneity of the prostate stromal cells is widely appreciated but the functional implication remains incompletely understood. Using genetic lineage tracing and light-sheet imaging, we show that some fibroblast cells near the junction of the mouse proximal prostatic ducts and prostatic urethra highly express Lgr5. Genetic ablation of these anatomically restricted stromal cells, but not nonselective ablation of prostatic stromal cells, rapidly induces prostate epithelial turnover and dedifferentiation that are reversed following spontaneous restoration of the Lgr5+ stromal cells. These changes are not mediated via endocrine or nervous regulation but can be phenocopied by physical disruption of prostatic lumen integrity. RNA-Seq analysis implies that ablating the Lgr5+ stromal cells activates a mechanosensory response, which is supported by increased prostate tissue stiffness and activation of the mitogen-activated protein kinase (MAPK). Suppressing MAPK overrides the increased epithelial proliferation. This study demonstrates that the Lgr5+ stromal cells regulate tissue homeostasis in a long-distance manner by maintaining anatomic integrity and implies that the cells near the transitional regions between organs likely control organ homeostasis by sustaining a balanced mechanoforce.

2023-01-06 | GSE190938 | GEO

SCA-1 Labels a Subset of Estrogen-Responsive Bipotential Repopulating Cells within the CD24<sup>+</sup> CD49f<sup>hi</sup> Mammary Stem Cell-Enriched Compartment.

Project description: Not available

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Gene expression profile of zebrafish renal collecting ducts

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Genome-wide analysis of prostatic tissue gene expression from patients with benign prostatic hyperplasia

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Gene expression profile and functionality of ESC-derived Lin-ckit+Sca-1+ cells are distinct from Lin-ckit+Sca-1+ cells isolated from fetal liver or bone marrow.

Project description: Not available

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