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Response of peripheral blood mononuclear cells from 20 healthy donor and 15 patients with type 1 diabetes to type 1 diabetogenic protein (IGRP, PPI) derived peptide stimulation.

ABSTRACT: CD4 T cell responses are characterized based on a limited number of molecular markers selected from exisiting knowledge. The goal of the experiment was to assess antigenic-peptide specific T-cell responses in vitro without bias using microarrays.

ORGANISM(S): Homo sapiens

PROVIDER: GSE72377 | GEO | 2016/08/15

SECONDARY ACCESSION(S): PRJNA293902

REPOSITORIES: GEO

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Response of peripheral blood mononuclear cells from 20 healthy donor and 15 patients with type 1 diabetes to type 1 diabetogenic protein (IGRP, PPI) derived peptide stimulation.

Project description:CD4 T cell responses are characterized based on a limited number of molecular markers selected from exisiting knowledge. The goal of the experiment was to assess antigenic-peptide specific T-cell responses in vitro without bias using microarrays. PBMCs were isolated from 20 healthy donors and 15 patients with type 1 diabetes. The cells were stimulated with peptides derived from type 1 diabetogenic protein (IGRP, PPI) for 24 hours. Unstimulated cells were cultured without the peptides for 24 hours.

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