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Embryonic stem cells culture conditions support distinct ES cell states associated with specific developmental stages and cell types

ABSTRACT: Identification of transcripts in murine embryonic stem (ES) cell lines growing under 3 different self-renewing conditions; GMEM + 10% FCS, N2B27 + 2i (1 μM PD032 and 3 μM CHIR99021) and knockout serum replacement (KOSR) medium (80% Knockout DMEM, 20% Knockout Serum Replacement). Under all conditions, ES cells were grown on gelatin-coated dishes and passaged by trypsinisation. ES cells were cultured in each condition for at least 3 passages prior to sample collection. The aim of this array experiment is to identify significant differences in transcript levels of ES cells grown under different conditions. Differences of transcript levels from the different conditions should be consistent among the biological and technical replicates for each.

ORGANISM(S): Mus musculus

PROVIDER: GSE72445 | GEO | 2016/07/14

SECONDARY ACCESSION(S): PRJNA294077

REPOSITORIES: GEO

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