Transcriptomics,Genomics

Dataset Information

26

Δ9-tetrahydrocannabinol (Δ9-THC) promotes neuroimmune-modulatory microRNA profile in striatum of simian immunodeficiency virus (SIV)-infected macaques.


ABSTRACT: Cannabinoid administration before and after simian immunodeficiency virus (SIV)-inoculation ameliorated disease progression and decreased inflammation in male rhesus macaques. Δ9-tetrahydrocannabinol (Δ9-THC) did not increase viral load in brain tissue or produce additive neuropsychological impairment in SIV-infected macaques. To determine if the neuroimmunomodulation of Δ9-THC involved differential microRNA (miR) expression, miR expression in the striatum of uninfected macaques receiving vehicle (VEH) or Δ9-THC (THC) and SIV-infected macaques administered either vehicle (VEH/SIV) or Δ9-THC (THC/SIV) was profiled using next generation deep sequencing. Overall design: Brains were removed, flash frozen on dry ice, and RNA was harvested using Norgen RNA extraction kit. Illumina TruSeq RNA Sample Prep Kit was used with 500 ng of total RNA for the construction of sequencing libraries.

INSTRUMENT(S): Illumina Genome Analyzer IIx (Macaca mulatta)

SUBMITTER: Liz Simon  

PROVIDER: GSE72766 | GEO | 2016-01-22

SECONDARY ACCESSION(S): PRJNA295016

REPOSITORIES: GEO

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Publications

Δ9-Tetrahydrocannabinol (Δ9-THC) Promotes Neuroimmune-Modulatory MicroRNA Profile in Striatum of Simian Immunodeficiency Virus (SIV)-Infected Macaques.

Simon Liz L   Song Keijing K   Vande Stouwe Curtis C   Hollenbach Andrew A   Amedee Angela A   Mohan Mahesh M   Winsauer Peter P   Molina Patricia P  

Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology 20151125 1


Cannabinoid administration before and after simian immunodeficiency virus (SIV)-inoculation ameliorated disease progression and decreased inflammation in male rhesus macaques. Δ9-tetrahydrocannabinol (Δ9-THC) did not increase viral load in brain tissue or produce additive neuropsychological impairment in SIV-infected macaques. To determine if the neuroimmunomodulation of Δ9-THC involved differential microRNA (miR) expression, miR expression in the striatum of uninfected macaques receiving vehicl  ...[more]

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