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Mytilus galloprovincialis, digestive gland and gill, exposed to Prorocentrum lima, producers of okadaic acid.

ABSTRACT: Differential expression analysis of digestive gland and gill tissues of mussels (Mytilus galloprovincialis) exposed to dinoflagellates (Prorocentrum lima), producers of okadaic acid, at a concentration of 200 cells/ml for one day. Each sample consists in total RNA was extracted from pooled tissues of 5 individuals.

ORGANISM(S): Mytilus galloprovincialis

PROVIDER: GSE72817 | GEO | 2015/11/19

SECONDARY ACCESSION(S): PRJNA295140

REPOSITORIES: GEO

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Mytilus galloprovincialis, digestive gland and gill, exposed to Prorocentrum lima, producers of okadaic acid.

Project description:Differential expression analysis of digestive gland and gill tissues of mussels (Mytilus galloprovincialis) exposed to dinoflagellates (Prorocentrum lima), producers of okadaic acid, at a concentration of 200 cells/ml for one day. Each sample consists in total RNA was extracted from pooled tissues of 5 individuals. Two-color dye-swap direct comparison experiment: exposed vs non-exposed (treated vs control). Biological replicates: two treated replicates and two control replicates per tissue.

2015-11-19 | E-GEOD-72817 | biostudies-arrayexpress

Mytilus galloprovincialis: temporal expression analysis of digestive gland and mantle

Project description:This SuperSeries is composed of the following subset Series: GSE22915: Mussel (Mytilus galloprovincialis) digestive gland tissue: gene expression profiles across an annual cycle GSE23049: Mytilus galloprovincialis: development of female gonads GSE23050: Mytilus galloprovincialis: development of male gonads GSE23051: Mytilus galloprovincialis: differences between male and female gene expression patterns in gonads (mantle tissue) Refer to individual Series

2011-07-11 | E-GEOD-23052 | biostudies-arrayexpress

Mytilus galloprovincialis

Project description:Mytilus galloprovincialis, digestive gland and gill, exposed to Prorocentrum lima, producers of okadaic acid.

| PRJNA295140 | ENA

Time course study of Mediterranean mussel (M. galloprovincialis, Lmk, 1819) digestive gland treated with okadaic acid

Project description:Mytilus galloprovincialis (Lmk, 1819) is economically relevant bivalve specie. In Adriatic Sea, periodical temperatures increases define optimal growth conditions for Dinoflagellate spp which can reach high concentrations also in filter-feeding mussels, thus causing potential human health problems. The most commonly used methods for the detection of Diarrhoeic Shellfish Poisoning biotoxins have either a low sensitivity or are too expensive to be used for routine tests. Genomic tools, such as microarray platforms, provide a reliable and alternative solution to overcome these problems. In this study we used a mussel cDNA microarray for studying gene expression changes in mussels exposed to Okadaic acid. Mussels collected in the Gulf of Trieste, located in Northern Adriatic Sea, were fed with Okadaic acid-spiked invertebrates for five weeks. In a time course experiment we were able to describe an early acute response just from the first 4th day time point. Among the differentially expressed genes we found a general up-regulation of stress proteins and proteins involved in cellular synthesis. Overall, we identified 34 transcripts candidate as useful markers to monitor OA-induced stress in mussels. This study contributes to the characterization of many potential genetic markers that could be used in future environmental monitoring, and could lead to explore new mechanisms of stress tolerance in marine mollusc species. Keywords: Time course, stress response

2009-02-19 | GSE14885 | GEO

Time course study of Mediterranean mussel (M. galloprovincialis, Lmk, 1819) digestive gland treated with okadaic acid

2010-05-05 | E-GEOD-14885 | biostudies-arrayexpress

Proteogenomics analysis of the digestive gland from the mussel Mytilus galloprovincialis exposed to carbamazepine

Project description:teogenomics analysis from digestive glands from Mytilus galloprovincialis exposed to two concentrations of carbamazepine.

2022-04-23 | PXD024201 | Pride

Mytilus galloprovincialis

Project description:Mytilus galloprovincialis overview

| PRJNA80319 | ENA

Mytilus galloprovincialis

Project description:Mytilus galloprovincialis Genome Survey

| PRJNA262617 | ENA

Mytilus galloprovincialis

Project description:Mytilus galloprovincialis Genome sequencing

| PRJNA246144 | ENA

Mussel (Mytilus galloprovincialis) digestive gland tissue: gene expression profiles across an annual cycle

Project description:Transcriptional profiling of natural population of mussels (Mytilus galloprovincialis) -digestive gland tissue- comparing female individuals sampled in the Bizerta Lagoon, Tunisia, across May 2007 - April 2008. Background: Seasonal environmental changes may affect the physiology of Mytilus galloprovincialis (Lam.), an intertidal filter-feeder bivalve occurring commonly in Mediterranean and Atlantic coastal areas. We investigated seasonal variations in relative transcript abundance of the digestive gland and the mantle (gonads) of males and females. To identify gene expression trends, we used a medium-density cDNA microarray (1.7 K probes) in dual-color competitive hybridization analyses. Results: Hierarchical clustering of digestive gland microarray data showed two main branches, distinguishing profiles associated with the “hot” months (May–August) from the other months. Genes involved in chitin metabolism, associated with mussel nutrition and digestion, showed higher expression during summer. Moreover, we found different gene expression patterns in the digestive glands of males and females during the four stages of mussel gonadal development. Microarray data from gonadal transcripts also displayed clear patterns during the different developmental phases with peak relative mRNA abundance at the ripe phase (stage III) for both sexes. Conclusion: These data showed a clear temporal pattern in gene expression profiles of mussels sampled over an annual cycle. Physiological response to thermal variation, food availability, and reproductive status across months may contribute to variation in gene expression.

2011-06-06 | GSE22915 | GEO

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