Dataset Information


Expression analysis by RNAseq of roots and shoots of the pho1 mutant expressing the EXS domain of PHO1

ABSTRACT: Expression of the EXS domain of PHO1 in the Arabidopsis thaliana pho1 mutant leads to improved shoot growth in comparison to the parental pho1 mutant. We have used RNAseq to study the gene expression profile in the roots and shoots of the pho1 mutant, two independent transgenic lines of pho1 expressing the EXS domain of PHO1 (lines EXS32 and EXS34) as well as a pho1 mutant transformed with the full length PHO1 gene (complemented line). Overall design: Plants were grown for 25 days in pots, roots and shoots were harvested and RNA was extracted

INSTRUMENT(S): Illumina HiSeq 2500 (Arabidopsis thaliana)

SUBMITTER: Sylvain Pradervand  

PROVIDER: GSE73067 | GEO | 2016-01-15



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The EXS Domain of PHO1 Participates in the Response of Shoots to Phosphate Deficiency via a Root-to-Shoot Signal.

Wege Stefanie S   Khan Ghazanfar Abbas GA   Jung Ji-Yul JY   Vogiatzaki Evangelia E   Pradervand Sylvain S   Aller Isabel I   Meyer Andreas J AJ   Poirier Yves Y  

Plant physiology 20151106 1

The response of shoots to phosphate (Pi) deficiency implicates long-distance communication between roots and shoots, but the participating components are poorly understood. We have studied the topology of the Arabidopsis (Arabidopsis thaliana) PHOSPHATE1 (PHO1) Pi exporter and defined the functions of its different domains in Pi homeostasis and signaling. The results indicate that the amino and carboxyl termini of PHO1 are both oriented toward the cytosol and that the protein spans the membrane  ...[more]

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