Genomics

Dataset Information

40

Convergent evolution of peptide-based quorum sensing required for virulence in a eukaryotic pathogen


ABSTRACT: Bacterial quorum sensing (QS) systems are characterized by the regulated biogenesis and sensing of specialized signaling molecules. Here, we describe a peptide, Qsp1, secreted by the fungal pathogen Cryptococcus neoformans. Mutants lacking Qsp1 are attenuated for infection, pulmonary accumulation, and growth within macrophages. Qsp1 promotes density-dependent cell wall integrity and resistance to extreme environments. Qsp1 is also required for a secreted aspartyl protease activity required for virulence. Further biochemical and large-scale genetic investigations reveal that cleavage of Qsp1 from the C-terminal of a pro-peptide requires a cell-associated serine protease and Qsp1 sensing requires a predicted oligopeptide importer. Strikingly, these features closely mirror the hallmarks of a peptide-based QS system found in gram-positive bacteria, despite no ancestral relationship between individual components. Our studies thus reveal a convergently evolved, peptide-based QS system required for the virulence of a fungal pathogen. Overall design: RNA-Seq or ChIP-Seq to compare multiple Cryptococcus neoformans genotypes using the HiSeq 2500 platform

INSTRUMENT(S): Illumina HiSeq 2500 (Cryptococcus neoformans)

SUBMITTER: Christina M. Homer  

PROVIDER: GSE73203 | GEO | 2016-06-08

SECONDARY ACCESSION(S): PRJNA296366

REPOSITORIES: GEO

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Quorum sensing (QS) is a bacterial communication mechanism in which secreted signaling molecules impact population function and gene expression. QS-like phenomena have been reported in eukaryotes with largely unknown contributing molecules, functions, and mechanisms. We identify Qsp1, a secreted peptide, as a central signaling molecule that regulates virulence in the fungal pathogen Cryptococcus neoformans. QSP1 is a direct target of three transcription factors required for virulence, and qsp1Δ  ...[more]

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