Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Gene expression changes upon Lamin A and Lamin B2 depletion in a diploid colorectal cancer cell line - DLD1

ABSTRACT: Small interfering RNA (siRNA) mediated depletion of Lamin A/C and Lamin B2 was performed in a diploid colorectal cancer cell line - DLD1 with an aim to identify transcription deregulation across the genome.

ORGANISM(S): Homo sapiens

PROVIDER: GSE73269 | GEO | 2016/09/24

SECONDARY ACCESSION(S): PRJNA296495

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

DLD1 Diploid vs Tetraploid

Project description:Tetraploidisation has been implicated in tumorigenesis and in aneuploidy which is frequently observed in most human cancers. In this study we aim to reveal differences in gene expression profile of p53 defective isogenic diploid and tetraploid cell populations. Isogenic DLD1 populations were obtained by transient Dihydro-Cytochalasin B treatment and cell sorting of pure diploid and tetraploid populations by cell cycle profile several days after release from DCB. RNA isolation: Two technical replicas were collected for each population from two different passages (biological replicas) of 60-80% confluent cells.

2014-04-22 | E-MTAB-1789 | biostudies-arrayexpress

Lamin B2 controls nuclear envelope permeability and regulates cardiomyocyte regeneration

Project description:Lamin B2 controls nuclear envelope permeability and regulates cardiomyocyte regeneration

| PRJNA423262 | ENA

DLD1

Project description:DLD1 is an APC mut, KRAS mut, P53 mut CRC cell line. PROX1 transcription factor, target of Wnt pathway in CRC, is our protein of interest.DLD1 cells are PROX1 negative. We overexpressed through lentiviral expression PROX1 protein or the empty vector psd44, through selection of the cells in puromycin resistance. Afterwards we compared the transcriptional program of the DLD1-PROX1 and DLD1-Control cells growing in monolayer in vitro.

2014-09-18 | E-MTAB-2020 | biostudies-arrayexpress

Lamin B2 controls nuclear envelope permeability and regulates cardiomyocyte regeneration

Project description:Heart muscle cells, cardiomyocytes, are highly differentiated cells that usually do not proliferate . During the non-proliferative state, extracellular signals control cardiomyocyte contractile function. However, during development and regeneration, cardiomyocytes enter the cell cycle and divide. It is unknown how cardiomyocytes modify their intracellular signaling to direct the cell cycle program. Here, we show that the nuclear lamina protein Lamin B2 (Lmnb2) regulates cardiomyocyte cell cycle activity using a gatekeeper mechanism. We identified Lmnb2 as a candidate for regulating intracellular signaling with deep transcriptional profiling of single cardiomyocytes. Lmnb2 was sufficient and necessary for cardiomyocyte cycling in the presence of serum. Lmnb2 increased the nucleoporin NUP98 and permeability of the nuclear membrane for phosphorylated ERK1/2. In vivo, the Lmnb2 gene was required for cardiomyocyte cell cycle activity during development. Increasing the expression of Lmnb2 in neonatal mice promoted cardiomyocyte M-phase and cytokinesis. LmnB2 gene transfer in neonatal mice that received a myocardial injury increased cardiomyocyte division and myocardial function in the injury border zone, indicating that the regenerated cardiomyocytes were functionally integrated. We propose a gatekeeper function of Lmnb2 that can be targeted to increase cardiomyocyte regeneration without the administration of exogenous growth factors.

2020-02-28 | GSE108360 | GEO

Effect of depletion of ZNF692 on gene expression in DLD1 colorectal human cells

Project description:To investigate the function of ZNF692 in colorectal cancer, we established DLD1 cell lines in which target gene has been knocked out by CRISPR-cas. We then performed gene expression profiling analysis using data obtained from DLD1 colorectal human cells wt (sgC for guide control) or crispr knockout for ZNF692 (sgZ1 for guide 1, and sgZ3 for guide 3). We have each condition in triplicates.

2022-10-17 | GSE215838 | GEO

Stochastic genome-nuclear lamina interactions: Modulating roles of Lamin A and BAF

Project description:The nuclear lamina (NL) is a filamentous layer lining the inner-nuclear-membrane (INM) that aids in the organization of the genome in large domains of low transcriptional activity. Recently, it was shown that the single-cell genome-NL interactions are much more dynamic than previously anticipated, which challenges the concept of the NL as a safe guard for transcriptional repressed genes. Here we discuss the role of the NL in light of these new findings and introduce Lamin A and BAF as potential modulators of LAD positioning BAF-chromatin and Lamin B2-chromatin interactions were assayed in human HT1080 by DamID on Nimblegen microarrays, with two biological replicates each, that were hybridized in a dye-swap design.

2014-02-15 | E-GEOD-55066 | biostudies-arrayexpress

Genome-wide maps of chromatin state of H3K27me3 in DLD1 parental cells and DLD1 p85β K477A/R478A mutant cells [ChIP-seq]

Project description:We performed ChIP coupled with high-throughput sequencing (ChIP-seq) for H3K27me3 in DLD1 parental cells and DLD1 p85β K477A/R478A mutant cells

2021-12-11 | GSE190434 | GEO

Next Generation Sequencing Facilitates Quantitative Analysis of DLD1 parental cells and DLD1 p85β K477A/R478A mutant cells [RNA-seq]

Project description:To elucidate whether p85β nuclear translocation plays a role in cancer tumorigenesis, a RNA-seq analysis was performed to compare the gene expression profiles of DLD1 parental cells and DLD1 p85β K477A/R478A mutant cells

2021-12-15 | GSE190740 | GEO

Expression data from DLD1 cells following H19 knockdown

Project description:Knockdown of H19 leads to cell cycle arrest, reduced cell proliferation, and reduced cell migration in DLD1 cells. We used microarrays to detail the global programme of gene expression following H19 knockdown in DLD1 cells

2016-09-29 | GSE87427 | GEO

Difference of gene expression among DLD1, oxaliplatin resistant clones derived from DLD1, HCT116, and oxaliplatin resistant clones derived from HCT116

Project description:Gene expression between DLD1 and DLD1 derived oxaliplatin resistant clones (DLD/OHP1, DLD/OHP4, and DLD/OHP5) was assessed Gene expression between HCT116 and HCT116 derived oxaliplatin resistant clones (HCT/OHP1, HCT/OHP3, and HCT/OHP5) was assessed

2020-05-31 | GSE77932 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data