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HTERT promotes cell adhesion and migration independent of telomerase activity

ABSTRACT: We sequenced mRNA from 6 human cell lines stably over-expressed specific gene or empty vector, and searched for differently expressed genes after gene over-expression as compared to empty vector.

ORGANISM(S): Homo sapiens

PROVIDER: GSE73277 | GEO | 2015/09/22

SECONDARY ACCESSION(S): PRJNA296510

REPOSITORIES: GEO

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hTERT promotes cell adhesion and migration independent of telomerase activity

Project description:We sequenced mRNA from 6 human cell lines stably over-expressed specific gene or empty vector, and searched for differently expressed genes after gene over-expression as compared to empty vector. mRNA levels were compared as following pairs: U2OS+hTERT vs U2OS+vector; U2OS+hTERTmut vs U2OS+vector; VA-13+hTERT vs VA-13+vector; VA-13+hTERTmut vs VA-13+vector.

2015-09-22 | E-GEOD-73277 | biostudies-arrayexpress

hTERT promotes cell adhesion and migration independent of telomerase activity.

Project description: Not available

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Using small RNA-Seq to verify that 5' tRNA-fMet1 half is over-expressed in outer membrane vesicles (OMVs)

Project description:5′ tRNA-fMet1 half (5′- CGCGGGGTGGAGCAGTCTGGTAGCTCGTCGGGCTC-3′) was cloned into the arabinose-inducible expression vector pMQ70. PA14 was transformed with the tRNA-fMet1 half expression vector or empty vector via electroporation. Characterization of the sRNA content of OMVs secreted by 5' tRNA-fMet1 half over-expression clone and empty vector control clone.

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Homo sapiens

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Project description:We did transcription profiling on the effect of RCK1 over-expression. rck1 mutant strain was transformed with empty high copy vector pRS425 empty vector), or with RCK1 cloned into pRS425 (the RCK1-overexpressing strain: RCK1 cloned into pRS425).

2013-07-31 | GSE49340 | GEO

Force transmission during adhesion-independent migration.

Project description: Not available

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Transcription profiling of human mammary epithelial cells transduced with either hTERT, empty LXSN vector or empty BABE vector

Project description:Analysis of gene expression in mammary epithelial cells transduced with either hTERT, empty LXSN vector or empty BABE vector.

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Rhodobacter sphaeroides 2.4.1 pRKSorX144 versus pRK415 under singlet oxygen stress.

Project description:To learn more about the function of SorX, we constructed an over-expression strain. For this purpose, the sorX gene together with its 28-bp upstream region was cloned into the over-expression vector pRK4352 and expressed in the R. sphaeroides wild-type 2.4.1 background. The corresponding strain consequently exhibits a SorX over-expression that is driven by the constitutive 16S rRNA (RSP_4352) promoter. The over-expression strain was compared to an empty vector control (pRK415). Total RNA from the over-expression (pRKSorX144) and from the empty vector control (pRK415) strain was extracted after 7 min of singlet oxygen stress and used for microarray analysis to investigate SorX-induced changes on transcriptome level.

2017-01-01 | GSE79421 | GEO

Phenotypic characterisation of LRRN4CL over expression

Project description:This study aims to use RNAseq to identify differentially expressed transcripts in human melanoma cells that over-express the cell surface protein, LRRN4CL, relative to empty-vector control cells, to provide mechanistic insight into how LRRN4CL over-expression confers enhanced pulmonary metastatic colonisation abilities.

| EGAS00001003976 | EGA

EGAS00001003976-sc-2020-07-01T08:56:44Z - samples

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